{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:24:16Z","timestamp":1791905056143,"version":"4.3.4"},"reference-count":18,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5574","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2002,6,7]]},"abstract":"<jats:p>Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)\u2013peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte\u2013stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.<\/jats:p>","DOI":"10.1126\/science.1070945","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:54:32Z","timestamp":1027749272000},"page":"1876-1880","source":"Crossref","is-referenced-by-count":242,"title":["Dynamics of Thymocyte-Stromal Cell Interactions Visualized by Two-Photon Microscopy"],"prefix":"10.1126","volume":"296","author":[{"given":"Philippe","family":"Bousso","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirav R.","family":"Bhakta","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard S.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Robey","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00209-0"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00208-9"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(97)80144-6"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321027"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.3.845"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.4049\/jimmunol.153.5.1915","volume":"153","author":"Anderson G.","year":"1994","unstructured":"Anderson G., Owen J. J., Moore N. C., Jenkinson E. J., J. Immunol. 153, 1915 (1994).","journal-title":"J. Immunol."},{"key":"e_1_3_1_8_2","unstructured":"Supplementary material is available on Science Online."},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1155\/1993\/98015"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/9.3.381"},{"key":"e_1_3_1_11_2","unstructured":"P. Bousso unpublished data."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00032-7"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.12.2133"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19970801)38:3<250::AID-JEMT6>3.0.CO;2-Q"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.3.390-403.2001"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/25764"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.221"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.12.1909"},{"key":"e_1_3_1_19_2","unstructured":"We thank B. J. Fowlkes D. Raulet and members of the Robey laboratory for critical reading of the manuscript; J. Owen for advice on preparing RTOC cultures; and S. Smith and N. O'Rourke for help in designing and constructing the two-photon microscope. Supported by NIH grants AI32985 and AI42033 (E.R.) and GM45374 (R.S.L.). P.B. is supported by the Cancer Research Institute. N.R.B. is supported by the Medical Scientist Training Program at Stanford. Supplementary Online Material www.sciencemag.org\/cgi\/content\/full\/296\/5574\/1876\/DC1 SFigs. 1 to S3 Movies S1 to S11"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1070945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:50:24Z","timestamp":1704840624000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1070945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6,7]]},"references-count":18,"journal-issue":{"issue":"5574","published-print":{"date-parts":[[2002,6,7]]}},"alternative-id":["10.1126\/science.1070945"],"URL":"https:\/\/doi.org\/10.1126\/science.1070945","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1007175.90568","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1007175.92357","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6,7]]}}}