{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:42:30Z","timestamp":1792374150613,"version":"4.4.0"},"reference-count":22,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5608","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,2,7]]},"abstract":"<jats:p>\n                    Nitric oxide was found to trigger mitochondrial biogenesis in cells as diverse as brown adipocytes and 3T3-L1, U937, and HeLa cells. This effect of nitric oxide was dependent on guanosine 3\u2032,5\u2032-monophosphate (cGMP) and was mediated by the induction of peroxisome proliferator\u2013activated receptor \u03b3 coactivator 1\u03b1, a master regulator of mitochondrial biogenesis. Moreover, the mitochondrial biogenesis induced by exposure to cold was markedly reduced in brown adipose tissue of endothelial nitric oxide synthase null-mutant (eNOS\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    ) mice, which had a reduced metabolic rate and accelerated weight gain as compared to wild-type mice. Thus, a nitric oxide\u2013cGMP\u2013dependent pathway controls mitochondrial biogenesis and body energy balance.\n                  <\/jats:p>","DOI":"10.1126\/science.1079368","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T21:06:43Z","timestamp":1044565603000},"page":"896-899","source":"Crossref","is-referenced-by-count":1059,"title":["Mitochondrial Biogenesis in Mammals: The Role of Endogenous Nitric Oxide"],"prefix":"10.1126","volume":"299","author":[{"given":"Enzo","family":"Nisoli","sequence":"first","affiliation":[{"name":"Department of Preclinical Sciences, Center for Study and Research on Obesity, Luigi Sacco Hospital, University of Milan, Milan 20157, Italy."},{"name":"Istituto Auxologico Italiano, Milan 20149, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Clementi","sequence":"additional","affiliation":[{"name":"DIBIT-H San Raffaele Institute, Milan 20132, Italy."},{"name":"Department of 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University College London, London WC1E 6BT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele O.","family":"Carruba","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Center for Study and Research on Obesity, Luigi Sacco Hospital, University of Milan, Milan 20157, Italy."},{"name":"Istituto Auxologico Italiano, Milan 20149, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0290751"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702131"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02245-7"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90385-3"},{"key":"e_1_3_1_6_2","unstructured":"Materials and methods are available as supporting material on Science 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thank A. Higgs for her help with preparation of the manuscript; L. Vizzotto for assistance with light microscopy; F. Gennari for help with figure preparation; R. Scurati for help with food intake and body weight measurements; and E. Ferrannini J. Meldolesi and P. Mantegazza for critical support and revision of the manuscript. Supported by grants from the Ministero dell'Istruzione dell'Universit\u00e0 e della Ricerca cofinanziamento 2001 to E.N. and E.C. the Italian Association for Cancer Research to E.C. the Medical Research Council to S.M. and the University of Milan and the Italian Ministry of Health to M.O.C. and E.N."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1079368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T23:17:36Z","timestamp":1704842256000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1079368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,7]]},"references-count":22,"journal-issue":{"issue":"5608","published-print":{"date-parts":[[2003,2,7]]}},"alternative-id":["10.1126\/science.1079368"],"URL":"https:\/\/doi.org\/10.1126\/science.1079368","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1011672.186468","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,2,7]]}}}