{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:32:00Z","timestamp":1792733520885,"version":"4.5.0"},"reference-count":27,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5608","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,2,7]]},"abstract":"<jats:p>\n                    The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-\n                    <jats:italic>myc<\/jats:italic>\n                    under control of the zebrafish\n                    <jats:italic>Rag2<\/jats:italic>\n                    promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-\n                    <jats:italic>myc<\/jats:italic>\n                    \u2013 induced carcinogenesis.\n                  <\/jats:p>","DOI":"10.1126\/science.1080280","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T21:06:43Z","timestamp":1044565603000},"page":"887-890","source":"Crossref","is-referenced-by-count":488,"title":["Myc-Induced T Cell Leukemia in Transgenic Zebrafish"],"prefix":"10.1126","volume":"299","author":[{"given":"David M.","family":"Langenau","sequence":"first","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Traver","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo A.","family":"Ferrando","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery L.","family":"Kutok","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon C.","family":"Aster","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Kanki","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ed","family":"Prochownik","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus S.","family":"Trede","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard I.","family":"Zon","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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U.S.A."},{"key":"e_1_3_1_27_2","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1016\/S0960-9822(01)00482-1","volume":"11","author":"Peterson R. T.","year":"2001","unstructured":"Peterson R. T., Mably J. D., Chen J. N., Fishman M. C., Curr. Biol. 11, 1481 (2001).","journal-title":"Curr. Biol."},{"key":"e_1_3_1_28_2","unstructured":"We thank Y. Yang and M. Loda for paraffin sectioning and in situ analysis; J. Gilbert for editorial review; R. DePinho for the mouse myc cDNA; A. Winzeler for help with irradiation protocols; J. Morris for help with in vitro fertilization protocols; J. Vinokur G. Kourkoulis and W. Saganic for fish care and husbandry; and D. Dodson for manuscript preparation. Supported by NIH grants CA-68484 (A.T.L.) and CA-06516 (J.L.K. and J.C.A.). D.M.L. is an NSF predoctoral fellow D.T. is supported by the Irvington Institute for Immunological Research and A.A.F. is a fellow of the Leukemia and Lymphoma Society."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1080280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T23:16:27Z","timestamp":1704842187000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1080280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,7]]},"references-count":27,"journal-issue":{"issue":"5608","published-print":{"date-parts":[[2003,2,7]]}},"alternative-id":["10.1126\/science.1080280"],"URL":"https:\/\/doi.org\/10.1126\/science.1080280","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1012075.184235","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,2,7]]}}}