{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:19:52Z","timestamp":1791263992476,"version":"4.1.0"},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5617","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,4,11]]},"abstract":"<jats:p>\n                    Although primary CD8 responses to acute infections are independent of CD4 help, it is unknown whether a similar situation applies to secondary responses. We show that depletion of CD4 cells during the recall response has minimal effect, whereas depletion during the priming phase leads to reduced responses by memory CD8 cells to reinfection. Memory CD8 cells generated in CD4\n                    <jats:sup>+\/+<\/jats:sup>\n                    mice responded normally when transferred into CD4\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    hosts, whereas memory CD8 cells generated in CD4\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice mounted defective recall responses in CD4\n                    <jats:sup>+\/+<\/jats:sup>\n                    adoptive hosts. These results demonstrate a previously undescribed role for CD4 help in the development of functional CD8 memory.\n                  <\/jats:p>","DOI":"10.1126\/science.1082305","type":"journal-article","created":{"date-parts":[[2003,4,10]],"date-time":"2003-04-10T16:24:47Z","timestamp":1049991887000},"page":"337-339","source":"Crossref","is-referenced-by-count":1147,"title":["Requirement for CD4 T Cell Help in Generating Functional CD8 T Cell Memory"],"prefix":"10.1126","volume":"300","author":[{"given":"Devon J.","family":"Shedlock","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri778"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/30989"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/30996"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/31002"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.24.12950"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072615"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2053"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.12.8056-8063.1994"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.2.1072-1079.1996"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/353180a0"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.083001.084359"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1999.tb01326.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.1.348-352.1993"},{"key":"e_1_3_1_15_2","unstructured":"Material and methods are available as supporting material on Science Online."},{"key":"e_1_3_1_16_2","unstructured":"LCMV GP33-41 contains two major histocompatibility complex (MHC) class I\u2013restricted epitopes D b \/GP33-41 and K b \/GP34-41. Intracellular interferon-\u03b3 (IFN-\u03b3) staining following in vitro stimulation with GP33-41 peptide detects CD8 cells specific to both D b \/GP33-41 and K b \/GP34-41. Recombinant VV expressing the GP33-41 epitope (MG34) was provided by J. L. Whitton (Scripps La Jolla CA) and is referred to for clarity as \u201crVV33\u201d in this report."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1528"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040575197"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.7.3477"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.12.2205"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.20.9762-9765.2000"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01441"},{"key":"e_1_3_1_24_2","unstructured":"We thank C. A. Hunter J. L. Whitton and J. D. Altman for providing reagents and E. J. Pearce M. Yuk P. Bates and members of the Shen laboratory for discussion and critical reading of this manuscript. Supported by NIH grant AI45025 (H.S.) and NIH training grant AI07632 (D.J.S.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1082305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T21:27:37Z","timestamp":1704835657000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1082305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,11]]},"references-count":23,"journal-issue":{"issue":"5617","published-print":{"date-parts":[[2003,4,11]]}},"alternative-id":["10.1126\/science.1082305"],"URL":"https:\/\/doi.org\/10.1126\/science.1082305","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1013631.191506","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,11]]}}}