{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:44:23Z","timestamp":1791171863526,"version":"4.1.0"},"reference-count":17,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5630","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,7,11]]},"abstract":"<jats:p>We report the use of genetically engineered cells in a pathogen identification sensor. This sensor uses B lymphocytes that have been engineered to emit light within seconds of exposure to specific bacteria and viruses. We demonstrated rapid screening of relevant samples and identification of a variety of pathogens at very low levels. Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications.<\/jats:p>","DOI":"10.1126\/science.1084920","type":"journal-article","created":{"date-parts":[[2003,7,10]],"date-time":"2003-07-10T17:18:38Z","timestamp":1057857518000},"page":"213-215","source":"Crossref","is-referenced-by-count":232,"title":["A B Cell-Based Sensor for Rapid Identification of Pathogens"],"prefix":"10.1126","volume":"301","author":[{"given":"Todd H.","family":"Rider","sequence":"first","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha S.","family":"Petrovick","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances E.","family":"Nargi","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. 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Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"Mathews","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura T.","family":"Bortolin","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert M.","family":"Young","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhu","family":"Chen","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Hollis","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac981425v"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5413.449"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1989.tb09202.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2610913"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.2.601"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.21.8107"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2611"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(93)90091-J"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.5.1103"},{"key":"e_1_3_1_11_2","unstructured":"Materials and methods are available as supporting material on Science Online."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/515323"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(97)00054-8"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.8.1329"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.5.1769-1776.2000"},{"key":"e_1_3_1_16_2","first-page":"1151","volume":"46","year":"2000","unstructured":"F. Pourahmadi et al., Clin. Chem.46, 1151 (2000).","journal-title":"Clin. Chem."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90343-5"},{"key":"e_1_3_1_18_2","unstructured":"We appreciate the following generous donations: M12g3R B cell line P. Tucker (University of Texas Austin); pCMV.AEQ.IRES.NEO plasmid D. Button (Stanford University); VKExpress Invitrogen Corporation; B. anthracis Sterne antibody to orthopoxvirus and TC-83 VEE G. Ludwig (U.S. Army Medical Research Institute of Infectious Diseases); antibody to Y. pestis sequence and inactivated bacteria Naval Medical Research Center; antibody to B. anthracis spore J. Kearney (University of Alabama Birmingham); and antibody to VEE and inactivated VEE J. Roehrig (Centers for Disease Control and Prevention Fort Collins CO). We are grateful for the advice and assistance of M. Boes B. Cho L. Filip S. Lakdawala A. Lee K. Molvar S. T. Palmacci G. Paradis L. Parameswaran T. Schmidt C. Wang and C. Whitehurst. We greatly appreciate the assistance and support of E. Henchal A. Mateczun L. Peruski and J. Gebhardt. This work was sponsored by A. Rudolph of the Defense Advanced Research Projects Agency's Defense Sciences Office and by A. Senecal of the U.S. Army Soldier and Biological Chemical Command's Combat Feeding Program under Air Force contract number F19628-00-C-0002. Opinions interpretations conclusions and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1084920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T21:49:58Z","timestamp":1704836998000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1084920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,11]]},"references-count":17,"journal-issue":{"issue":"5630","published-print":{"date-parts":[[2003,7,11]]}},"alternative-id":["10.1126\/science.1084920"],"URL":"https:\/\/doi.org\/10.1126\/science.1084920","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1014368.201736","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1014368.193402","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7,11]]}}}