{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:12:28Z","timestamp":1791249148713,"version":"4.1.0"},"reference-count":39,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5635","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,8,15]]},"abstract":"<jats:p>\n            Genes associated with Hirschsprung disease, a failure to form enteric ganglia in the hindgut, were highly up-regulated in gut neural crest stem cells relative to whole-fetus RNA. One of these genes, the glial cell line\u2013derived neurotrophic factor (GDNF) receptor\n            <jats:italic>Ret<\/jats:italic>\n            , was necessary for neural crest stem cell migration in the gut. GDNF promoted the migration of neural crest stem cells in culture but did not affect their survival or proliferation. Gene expression profiling, combined with reverse genetics and analyses of stem cell function, suggests that Hirschsprung disease is caused by defects in neural crest stem cell function.\n          <\/jats:p>","DOI":"10.1126\/science.1085649","type":"journal-article","created":{"date-parts":[[2003,8,14]],"date-time":"2003-08-14T23:28:56Z","timestamp":1060903736000},"page":"972-976","source":"Crossref","is-referenced-by-count":184,"title":["Hirschsprung Disease Is Linked to Defects in Neural Crest Stem Cell Function"],"prefix":"10.1126","volume":"301","author":[{"given":"Toshihide","family":"Iwashita","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Internal Medicine and Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109\u20130934, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevieve M.","family":"Kruger","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Internal Medicine and Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109\u20130934, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Pardal","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Internal Medicine and Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109\u20130934, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Kiel","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Internal Medicine and Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109\u20130934, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean J.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Internal Medicine and Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109\u20130934, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1442"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131200898"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5471.1635"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00209-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.488"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072530"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073823"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212525299"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5485.1754"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0302-268"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00260-4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10024-001-0142-y"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80583-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00825-5"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00827-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81112-5"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-19-07370.2000"},{"key":"e_1_3_2_19_2","unstructured":"T. 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We thank D. Adams A. M. Deslaurier and M. White for flow cytometry (supported by NIH grants CA46592 to the UM Comprehensive Cancer Center and P60-AR20557 tothe UM Multipurpose Arthritis Center); E. Smith in the Hybridoma Core Facility [supported by NIH grants NIH5P60\u2013DK20572 tothe Michigan Diabetes Research and Training Center (MDRTC) and P30 AR48310 tothe Rheumatic Disease Core Center]; and A. McCallion and V. Pachnis for providing Ret -deficient mice. Microarray analysis was performed through the MDRTC (NIH DK58771) with assistance from D. Misek R. Koenig R. Kuick and K. Shedden."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1085649","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:15:05Z","timestamp":1704856505000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1085649"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8,15]]},"references-count":39,"journal-issue":{"issue":"5635","published-print":{"date-parts":[[2003,8,15]]}},"alternative-id":["10.1126\/science.1085649"],"URL":"https:\/\/doi.org\/10.1126\/science.1085649","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8,15]]}}}