{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:47:14Z","timestamp":1791341234221,"version":"4.3.1"},"reference-count":25,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5639","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,9,12]]},"abstract":"<jats:p>\n                    Stem cell self-renewal can be specified by local signals from the surrounding microenvironment, or niche. However, the relation between the niche and the mechanisms that ensure the correct balance between stem cell self-renewal and differentiation is poorly understood. Here, we show that dividing\n                    <jats:italic>Drosophila<\/jats:italic>\n                    male germline stem cells use intracellular mechanisms involving centrosome function and cortically localized Adenomatous Polyposis Coli tumor suppressor protein to orient mitotic spindles perpendicular to the niche, ensuring a reliably asymmetric outcome in which one daughter cell remains in the niche and self-renews stem cell identity, whereas the other, displaced away, initiates differentiation.\n                  <\/jats:p>","DOI":"10.1126\/science.1087795","type":"journal-article","created":{"date-parts":[[2003,9,12]],"date-time":"2003-09-12T01:03:39Z","timestamp":1063328619000},"page":"1547-1550","source":"Crossref","is-referenced-by-count":679,"title":["Orientation of Asymmetric Stem Cell Division by the APC Tumor Suppressor and Centrosome"],"prefix":"10.1126","volume":"301","author":[{"given":"Yukiko M.","family":"Yamashita","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."},{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Leanne","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."},{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret T.","family":"Fuller","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."},{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305\u20135329, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1427"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066707"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066700"},{"key":"e_1_3_1_5_2","unstructured":"Materials and methods are available as supporting material on Science Online."},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/71323"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.5.2123"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8669"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.11.3861"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81258-1"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00017-3"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/11064"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.6.1303"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1001-933"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/35054077"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.2.247"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(01)01954-7"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/35060140"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00176-2"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00119-3"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.2.377"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5461.2260"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0777"},{"key":"e_1_3_1_24_2","unstructured":"D. L. Jones et al . in preparation."},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.6.2061"},{"key":"e_1_3_1_26_2","unstructured":"We thank E. Wieschaus M. Bienz A. Spradling T. Uemura T. Kaufman H. Oda the Bloomington Stock Center and the Developmental Studies Hybridoma Bank for stocks and antibodies. We also thank T. Mahowald J. Nelson A. Barth and Fuller lab members for critical reading of the manuscript and discussions. Y.M.Y. is a Japan Society for the Promotion of Science fellow and D.L.J. is a Lilly fellow of the Life Sciences Research Foundation. Supported by NIH grant 1P01 DK53074 subproject 4 (M.T.F.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1087795","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T23:05:32Z","timestamp":1704841532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1087795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9,12]]},"references-count":25,"journal-issue":{"issue":"5639","published-print":{"date-parts":[[2003,9,12]]}},"alternative-id":["10.1126\/science.1087795"],"URL":"https:\/\/doi.org\/10.1126\/science.1087795","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1001476.197256","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.197958","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.196750","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.197428","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.198707","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.200160","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1001476.199379","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9,12]]}}}