{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:02:48Z","timestamp":1791842568663,"version":"4.3.4"},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5644","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,10,17]]},"abstract":"<jats:p>\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    is a Gram-positive intracytosolic pathogen that causes severe disease in pregnant and immunocompromised individuals. We found that\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    lacking the lipoate protein ligase LplA1 was defective for growth specifically in the host cytosol and was less virulent in animals by a factor of 300. A major target for LplA1, the E2 subunit of pyruvate dehydrogenase (PDH), lacked a critical lipoyl modification when the \u0394\n                    <jats:italic>lplA1<\/jats:italic>\n                    strain was grown intracellularly, which suggests that abortive growth was due to loss of PDH function. Thus, the use of host-derived lipoic acid may be a critical process for in vivo replication of bacterial pathogens.\n                  <\/jats:p>","DOI":"10.1126\/science.1088170","type":"journal-article","created":{"date-parts":[[2003,10,16]],"date-time":"2003-10-16T17:09:09Z","timestamp":1066324149000},"page":"462-464","source":"Crossref","is-referenced-by-count":141,"title":["<i>Listeria<\/i>\n                    Intracellular Growth and Virulence Require Host-Derived Lipoic Acid"],"prefix":"10.1126","volume":"302","author":[{"given":"Mary","family":"O'Riordan","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."},{"name":"Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA."},{"name":"Division of Infectious Disease, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlena A.","family":"Moors","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."},{"name":"Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA."},{"name":"Division of Infectious Disease, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."},{"name":"Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA."},{"name":"Division of Infectious Disease, School of Public Health, University of California, Berkeley, CA 94720\u20133202, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/mr.49.3.298-337.1985","volume":"49","year":"1985","unstructured":"J. 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Fink, Nature412, 83(2001).","journal-title":"Nature"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35021074"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067798"},{"key":"e_1_3_1_21_2","doi-asserted-by":"crossref","first-page":"3973","DOI":"10.1128\/IAI.70.7.3973-3977.2002","volume":"70","year":"2002","unstructured":"D. Dacheux et al., Infect. Immun.70, 3973 (2002).","journal-title":"Infect. Immun."},{"key":"e_1_3_1_22_2","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1128\/JB.182.8.2119-2124.2000","volume":"182","year":"2000","unstructured":"A. Stein, W. Firshein, J. Bacteriol.182, 2119 (2000).","journal-title":"J. Bacteriol."},{"key":"e_1_3_1_23_2","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1046\/j.1365-2958.2002.03072.x","volume":"45","year":"2002","unstructured":"L. L. Lenz, D. A. Portnoy, Mol. Microbiol.45, 1043 (2002).","journal-title":"Mol. Microbiol."},{"key":"e_1_3_1_24_2","unstructured":"We gratefully acknowledge members of the Portnoy laboratory for helpful discussions especially L. Lenz for critical review of the manuscript and sharing mass spectrometry results and B. Guo for technical support. We thank A. Decatur N. Andrews and R. Isberg for critical review of the manuscript. We are also grateful to M. Giedlin (Cerus Corp.) for the LD 50 data and L. Szweda (Case Western Reserve University) for the gift of the LA-specific antibody. This research was supported by NIH grants R01 AI27655 and AI29619 (D.A.P.) and by the Irvington Institute for Immunological Research (M.O.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1088170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T21:58:27Z","timestamp":1704837507000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1088170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10,17]]},"references-count":23,"journal-issue":{"issue":"5644","published-print":{"date-parts":[[2003,10,17]]}},"alternative-id":["10.1126\/science.1088170"],"URL":"https:\/\/doi.org\/10.1126\/science.1088170","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1004526.199615","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10,17]]}}}