{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:07:29Z","timestamp":1792678049745,"version":"4.5.0"},"reference-count":35,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5648","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2003,11,14]]},"abstract":"<jats:p>\n                    Nova proteins are neuron-specific antigens targeted in paraneoplastic opsoclonus myoclonus ataxia (POMA), an autoimmune neurologic disease characterized by abnormal motor inhibition.Nova proteins regulate neuronal pre-messenger RNA splicing by directly binding to RNA.To identify Nova RNA targets, we developed a method to purify protein-RNA complexes from mouse brain with the use of ultraviolet cross-linking and immunoprecipitation (CLIP).Thirty-four transcripts were identified multiple times by Nova CLIP.Three-quarters of these encode proteins that function at the neuronal synapse, and one-third are involved in neuronal inhibition.Splicing targets confirmed in Nova\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice include c-Jun N-terminal kinase 2, neogenin, and gephyrin; the latter encodes a protein that clusters inhibitory \u03b3-aminobutyric acid and glycine receptors, two previously identified Nova splicing targets.Thus, CLIP reveals that Nova coordinately regulates a biologically coherent set of RNAs encoding multiple components of the inhibitory synapse, an observation that may relate to the cause of abnormal motor inhibition in POMA.\n                  <\/jats:p>","DOI":"10.1126\/science.1090095","type":"journal-article","created":{"date-parts":[[2003,11,13]],"date-time":"2003-11-13T17:35:21Z","timestamp":1068744921000},"page":"1212-1215","source":"Crossref","is-referenced-by-count":1061,"title":["CLIP Identifies Nova-Regulated RNA Networks in the Brain"],"prefix":"10.1126","volume":"302","author":[{"given":"Jernej","family":"Ule","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk B.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Ruggiu","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Mele","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aljaz\u030c","family":"Ule","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Darnell","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA."},{"name":"Laboratory of Molecular Neuro-Oncology, Rockefeller University, New York, NY 10021, USA."},{"name":"Center for Research in Experimental Economics and Political Decision-Making, University of Amsterdam, 1018 WB Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90047-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-0172(95)80004-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5495"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.24.1.239"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/35049061"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.1.25"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00034-5"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.6.3194"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.23.11207"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14085"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80900-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00566-9"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00568-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090553997"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.22.13254"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80668-6"},{"key":"e_1_3_2_18_2","unstructured":"J. Ule K. B. Jensen R. B. Darnell data not shown."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.13.4687-4700.2003"},{"key":"e_1_3_2_20_2","unstructured":"M. Ruggiu B. K. Dredge R. B. Darnell unpublished data."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00636.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199605170-00021"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81336-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201225"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.18.10266"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.110128397"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161720"},{"key":"e_1_3_2_28_2","first-page":"8643","volume":"20","year":"2000","unstructured":"D. Billups, J. G. Hanley, M. Orme, D. Attwell, S. J. Moss, J. Neurosci.20, 8643 (2000).","journal-title":"J. Neurosci."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80381-5"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1582"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00332-4"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.18.5114"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5392.1321"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-21-09289.1999"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81165-4"},{"key":"e_1_3_2_36_2","unstructured":"K.B.J. developed the CLIP methodology. We thank N. Heintz L. Jan and H. Zoghbi for helpful comments on the manuscript and members of the lab especially J. C. Darnell B. K. Dredge and G. Stefani for their help and insight throughout this work. Supported by the NIH (K01 MH64753 to K.B.J. and R01 NS34389 and NS40955 to R.B.D.) the Howard Hughes Medical Institute a Human Frontiers Science Program fellowship (M.R.) and the tumor immunology program of Cancer Research Institute (J.U.). R.B.D. is an Investigator of the Howard Hughes Medical Institute."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1090095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:08:18Z","timestamp":1704838098000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1090095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,14]]},"references-count":35,"journal-issue":{"issue":"5648","published-print":{"date-parts":[[2003,11,14]]}},"alternative-id":["10.1126\/science.1090095"],"URL":"https:\/\/doi.org\/10.1126\/science.1090095","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1017007.201843","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,14]]}}}