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The \u03b1-helical segments associate to form three-stranded coiled coils, and surface-exposed interchain disulfide linkages mediate the formation of tail-to-tail hexamers. Analysis of serum samples shows that resistin circulates in two distinct assembly states, likely corresponding to hexamers and trimers. Infusion of a resistin mutant, lacking the intertrimer disulfide bonds, in pancreatic-insulin clamp studies reveals substantially more potent effects on hepatic insulin sensitivity than those observed with wild-type resistin. This result suggests that processing of the intertrimer disulfide bonds may reflect an obligatory step toward activation.<\/jats:p>","DOI":"10.1126\/science.1093466","type":"journal-article","created":{"date-parts":[[2004,5,20]],"date-time":"2004-05-20T19:48:13Z","timestamp":1085082493000},"page":"1154-1158","source":"Crossref","is-referenced-by-count":238,"title":["Disulfide-Dependent Multimeric Assembly of Resistin Family Hormones"],"prefix":"10.1126","volume":"304","author":[{"given":"Saurabh D.","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA."},{"name":"Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Rajala","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA."},{"name":"Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA."},{"name":"Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp E.","family":"Scherer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA."},{"name":"Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA."},{"name":"Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA."},{"name":"Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1001\/jama.288.14.1728","volume":"288","year":"2002","unstructured":"C. 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