{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:58:33Z","timestamp":1791298713933,"version":"4.1.0"},"reference-count":28,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5662","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2004,2,27]]},"abstract":"<jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm\/Dot type IVsecretion system is essential for its formation and maintenance. The\n                    <jats:italic>Legionella<\/jats:italic>\n                    genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm\/Dot\u2013dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the\n                    <jats:italic>Legionella<\/jats:italic>\n                    to commandeer a protozoan exocytic pathway for dissemination of the pathogen.\n                  <\/jats:p>","DOI":"10.1126\/science.1094226","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T17:14:31Z","timestamp":1077815671000},"page":"1358-1361","source":"Crossref","is-referenced-by-count":282,"title":["<i>Legionella<\/i>\n                    Effectors That Promote Nonlytic Release from Protozoa"],"prefix":"10.1126","volume":"303","author":[{"given":"John","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA."},{"name":"Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA."},{"name":"Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104\u20135046, USA."},{"name":"Department of Biology, Lamont-Doherty Earth Observatory, Columbia University, 61 Route 9W, Palisades, NY 10964\u20131000, USA."},{"name":"Department of Molecular Microbiology and Biotechnology, George S. 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Eukaryot. Microbiol.42, 1 (1995).","journal-title":"J. Eukaryot. Microbiol."},{"key":"e_1_3_1_29_2","unstructured":"We thank I. Rosenshine and I. Nisan of the Hebrew University Medical School in Ein Kerem Israel for plasmid pEX-CyaA and for helping us initiate the work with the CyaA cyclase. We thank R. Kessin G. Otto and M. Wu for help with Dictyostelium and helpful comments on the manuscript. We acknowledge the assistance of M. Reyes for plasmid construction and the use of microscopy equipment in the Oklahoma Medical Research Foundation imaging facility. This work was supported by grants: NIH-R01 AI23549 (H.A.S.); NSF MCB-0344541and OCAST-HR01-024 (M.C.); grant Nn. 1999011 from the United States\u2013Israel Binational Science Foundation (BSF) Jerusalem Israel (G.S.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1094226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:10:04Z","timestamp":1704838204000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1094226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,27]]},"references-count":28,"journal-issue":{"issue":"5662","published-print":{"date-parts":[[2004,2,27]]}},"alternative-id":["10.1126\/science.1094226"],"URL":"https:\/\/doi.org\/10.1126\/science.1094226","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1018743.211549","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,27]]}}}