none 10.1126/science.1094515 American Association for the Advancement of Science (AAAS) American Association for the Advancement of Science (AAAS) 221 10385932 5530 2024010917551400087 10.1126 2024-01-09T23:09:56Z 2004-02-16T20:16:03Z 507 10.3410/f.1017422.202965 Science Science 0036-8075 1095-9203 03 12 2004 303 5664 Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst Woo Suk Hwang College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Young June Ryu College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Jong Hyuk Park College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Eul Soon Park College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Eu Gene Lee College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Ja Min Koo College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Hyun Yong Jeon College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Byeong Chun Lee College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Sung Keun Kang College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Sun Jong Kim College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Curie Ahn College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Jung Hye Hwang College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Ky Young Park College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Jose B. Cibelli College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Shin Yong Moon College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea. Gachon Medical School, Incheon, 417-840, Korea. College of Medicine, Seoul National University, Seoul, 110-744, Korea. Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells. 03 12 2004 1669 1674 10.1126/science.1094515 10.1126/science.1094515 https://www.science.org/doi/10.1126/science.1094515 https://www.science.org/doi/pdf/10.1126/science.1094515 10.1126/science.282.5391.1145 10.1038/35042066 10.1038/12404 10.1038/nbt0798-642 10.1016/S0960-9822(00)00648-5 10.1002/1526-968X(200011/12)28:3/4<156::AID-GENE100>3.0.CO;2-T 10.1126/science.1059399 J. Regen. Med. 25 26 2001 J. B. Cibelli et al., J. Regen. Med.26, 25 (2001). 10.1016/S0015-0282(02)03209-0 Materials and methods are available as supporting material on Science Online. 10.1002/mrd.10309 10.1095/biolreprod.102.014886 Biol. Reprod. 1635 64 2004 B. Kuhholzer, R. J. Hawley, L. Lai, D. Kolber-Simonds, R. S. Prather, Biol. Reprod.64, 1635 (2004). 10.1038/242475a0 10.1017/S0967199401001083 10.1016/S0165-0378(01)00136-X 10.1016/S0015-0282(01)02854-0 10.1016/S0093-691X(02)00946-9 10.1002/(SICI)1098-2795(199601)43:1<105::AID-MRD13>3.0.CO;2-4 10.1038/76536 F. Mitelman An International System for Human Cytogenetic Nomenclature (S. Karger Basel Switzerland 1995). 10.1073/pnas.92.17.7844 10.1038/74447 10.1016/S0167-7799(02)02056-5 Proc. Natl. Acad. Sci. U.S.A. 1911 100 2003 K. E. Vranaet al., Proc. Natl. Acad. Sci. U.S.A.100 (suppl. 1), 1911 (2003). 10.1126/science.1082091 10.1038/385810a0 10.1073/pnas.192433399 10.1016/S0015-0282(01)01825-8 We thank Y. Y Hwang (Hanyang University) for assistance with oocyte collections; S. I. Rho (MizMedi Hospital) H. S. Yoon (MizMedi Hospital ) and S. K. Oh (Seoul National University) for assistance on hES cells culture; Y. K. Choi (Korea Research Institute of Bioscience and Biotechnology) for assistance on teratoma formation; Tak Ko (Michigan State University) for gene expression analysis of Cyno-1 cells; and A. Trounson (Monash University) B. D. Bavister (University of New Orleans) and D. P. Wolf (Oregon National Primate Research Center) for critical review of the manuscript. J. B. Cibelli made intellectual contributions to the manuscript and the RNA analysis of nonhuman primate cells. All human experiments were performed inKorea by Korean scientists. This study was supported by grants from Advanced Backbone IT Technology Development (grant IMT2000-C1-1) to W.S.H. and the Stem Cell Research Center (grant M102KL0100-02K1201-00223) to S.Y.M. The authors are grateful for a graduate fellowship provided by the Ministry of Education through the BK21 program.