{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:02:55Z","timestamp":1791352975605,"version":"4.3.1"},"reference-count":33,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5690","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2004,9,10]]},"abstract":"<jats:p>Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.<\/jats:p>","DOI":"10.1126\/science.1100367","type":"journal-article","created":{"date-parts":[[2004,9,9]],"date-time":"2004-09-09T17:17:55Z","timestamp":1094750275000},"page":"1615-1619","source":"Crossref","is-referenced-by-count":379,"title":["Activation of Endogenous Cdc42 Visualized in Living Cells"],"prefix":"10.1126","volume":"305","author":[{"given":"Perihan","family":"Nalbant","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599\u20137365, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Hodgson","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599\u20137365, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim","family":"Kraynov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599\u20137365, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei","family":"Toutchkine","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599\u20137365, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus M.","family":"Hahn","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599\u20137365, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00192-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4674"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.13.9725"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0290882"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/20726"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.29.18067"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003487200"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.19.13198"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60979-6"},{"key":"e_1_3_2_11_2","first-page":"1075","volume":"14","year":"1994","unstructured":"P. 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F. Palazzo, G. G. Gundersen, Sci. STKE2002, pe31 (2002).","journal-title":"Sci. STKE"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.43.26850"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/35015585"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.01.008"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/34208"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(95)80336-X"},{"key":"e_1_3_2_34_2","unstructured":"We are grateful to P. Hordijk G. Bokoch and L. Dehmelt for critical reading of the manuscript; to G. Bokoch for help with neutrophil stimulation assays; and to M. Berba for secretarial assistance. This work was funded by grants from the Deutsche Forschungsgemeinschaft (P.N.) the Arthritis Foundation (V.K.) the Leukemia and Lymphoma Society (A.T.) and by the NIH (grants GM57464 and GM64346) (K.M.H.). K.M.H. has a financial interest in and is a consultant for and member of the Scientific Advisory Board for Genospectra Company which seeks to develop products that precisely characterize molecular events that occur in cells."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1100367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T21:05:59Z","timestamp":1704834359000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1100367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,10]]},"references-count":33,"journal-issue":{"issue":"5690","published-print":{"date-parts":[[2004,9,10]]}},"alternative-id":["10.1126\/science.1100367"],"URL":"https:\/\/doi.org\/10.1126\/science.1100367","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1021147.243527","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1021147.254655","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1021147.241524","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1021147.243498","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,10]]}}}