{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:07:01Z","timestamp":1791274021910,"version":"4.1.0"},"reference-count":21,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5748","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2005,10,28]]},"abstract":"<jats:p>\n                    Many pathogenic bacteria use injectisomes to deliver effector proteins into host cells through type III secretion. Injectisomes consist of a basal body embedded in the bacterial membranes and a needle. In\n                    <jats:italic>Yersinia<\/jats:italic>\n                    , translocation of effectors requires the YopB and YopD proteins, which form a pore in the target cell membrane, and the LcrV protein, which assists the assembly of the pore. Here we report that LcrV forms a distinct structure at the tip of the needle, the tip complex. This unique localization of LcrV may explain its crucial role in the translocation process and its efficacy as the main protective antigen against plague.\n                  <\/jats:p>","DOI":"10.1126\/science.1118476","type":"journal-article","created":{"date-parts":[[2005,10,27]],"date-time":"2005-10-27T18:38:54Z","timestamp":1130438334000},"page":"674-676","source":"Crossref","is-referenced-by-count":310,"title":["The V-Antigen of\n                    <i>Yersinia<\/i>\n                    Forms a Distinct Structure at the Tip of Injectisome Needles"],"prefix":"10.1126","volume":"310","author":[{"given":"Catherine A.","family":"Mueller","sequence":"first","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Broz","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley A.","family":"Mu\u0308ller","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Ringler","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Erne-Brand","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Sorg","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Engel","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy R.","family":"Cornelis","sequence":"additional","affiliation":[{"name":"Biozentrum der Universita\u0308t Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."},{"name":"Maurice E. Mu\u0308ller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1322"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1046\/j.1365-2958.1997.2731623.x","volume":"23","year":"1997","unstructured":"G. R. Cornelis, H. Wolf-Watz, Mol. Microbiol.23, 861 (1997).","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1046\/j.1365-2958.1999.01408.x","volume":"32","year":"1999","unstructured":"J. Pettersson et al., Mol. Microbiol.32, 961 (1999).","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"5395","DOI":"10.1128\/IAI.67.10.5395-5408.1999","volume":"67","year":"1999","unstructured":"K. A. Fields, M. L. Nilles, C. Cowan, S. C. Straley, Infect. 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Dis."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1532-1542.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5363.602"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"17103","DOI":"10.1074\/jbc.M300091200","volume":"278","year":"2003","unstructured":"F. S. Cordes et al., J. Biol. Chem.278, 17103 (2003).","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1073\/pnas.071065798","volume":"98","year":"2001","unstructured":"E. Hoiczyk, G. Blobel, Proc. Natl. Acad. Sci. U.S.A.98, 4669 (2001).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1091422"},{"key":"e_1_3_2_15_2","unstructured":"Yersinia builds injectisomes when the temperature reaches 37\u00b0C the host's body temperature. Yop secretion is triggered by contact with a target cell or artificially by chelation of Ca 2+ ions ( 15 )."},{"key":"e_1_3_2_16_2","unstructured":"Materials and methods are available as supporting material on Science Online."},{"key":"e_1_3_2_17_2","unstructured":"Removal of lcrV leads to reduced synthesis of YopB and YopD because of a regulatory effect of LcrV on their expression. This undesired effect can be compensated by deleting yopQ ( 5 )."},{"key":"e_1_3_2_18_2","unstructured":"C. A. Mueller et al. unpublished data."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1128\/IAI.73.3.1432-1440.2005","volume":"73","year":"2005","unstructured":"W. L. Picking et al., Infect. Immun.73, 1432 (2005).","journal-title":"Infect. Immun."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1046\/j.1462-5822.2001.00168.x","volume":"3","year":"2001","unstructured":"S. J. Daniell et al., Cell. Microbiol.3, 865 (2001).","journal-title":"Cell. Microbiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1038\/sj.emboj.7600676","volume":"24","year":"2005","unstructured":"D. Chakravortty, M. Rohde, L. Jager, J. Deiwick, M. Hensel, EMBO J.24, 2043 (2005).","journal-title":"EMBO J."},{"key":"e_1_3_2_22_2","unstructured":"We thank P. Jen\u00f6 for mass spectrometry analyses M. Duerrenberger for use of the TEM facility and J. M. Meyer and J. Frey for supplying P. aeruginosa PAO1 and A. salmonicida JF2267. Supported by the Swiss National Science Foundation (grant nos. 32-65393.01 to G.C. and 3100-059415 to A.E.) and by the Maurice E. M\u00fcller Foundation of Switzerland."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1118476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T20:52:29Z","timestamp":1704833549000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1118476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,28]]},"references-count":21,"journal-issue":{"issue":"5748","published-print":{"date-parts":[[2005,10,28]]}},"alternative-id":["10.1126\/science.1118476"],"URL":"https:\/\/doi.org\/10.1126\/science.1118476","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1028877.341377","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1028877.343421","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10,28]]}}}