{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:43:11Z","timestamp":1791236591157,"version":"4.1.0"},"reference-count":19,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5755","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2005,12,16]]},"abstract":"<jats:p>Translesion synthesis (TLS) is the major pathway by which mammalian cells replicate across DNA lesions. Upon DNA damage, ubiquitination of proliferating cell nuclear antigen (PCNA) induces bypass of the lesion by directing the replication machinery into the TLS pathway. Yet, how this modification is recognized and interpreted in the cell remains unclear. Here we describe the identification of two ubiquitin (Ub)\u2013binding domains (UBM and UBZ), which are evolutionarily conserved in all Y-family TLS polymerases (pols). These domains are required for binding of pol\u03b7 and pol\u03b9 to ubiquitin, their accumulation in replication factories, and their interaction with monoubiquitinated PCNA. Moreover, the UBZ domain of pol\u03b7 is essential to efficiently restore a normal response to ultraviolet irradiation in xeroderma pigmentosum variant (XP-V) fibroblasts. Our results indicate that Ub-binding domains of Y-family polymerases play crucial regulatory roles in TLS.<\/jats:p>","DOI":"10.1126\/science.1120615","type":"journal-article","created":{"date-parts":[[2005,12,15]],"date-time":"2005-12-15T17:02:46Z","timestamp":1134666166000},"page":"1821-1824","source":"Crossref","is-referenced-by-count":639,"title":["Ubiquitin-Binding Domains in Y-Family Polymerases Regulate Translesion Synthesis"],"prefix":"10.1126","volume":"310","author":[{"given":"Marzena","family":"Bienko","sequence":"first","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Green","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Crosetto","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Rudolf","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Zapart","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Coull","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Kannouche","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Wider","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Peter","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan R.","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Dikic","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany."},{"name":"Genome Damage and Stability, University of Sussex, Falmer, Brighton BN1 9RQ, UK."},{"name":"Institute of Biochemistry, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Institute of Molecular Biology and Biophysics, ETH Ho\u0308nggerberg, 8093 Zu\u0308rich, Switzerland."},{"name":"Bioinformatics Group, Miltenyi Biotec GmbH, Stoeckheimer Weg 1, D-50829 Koeln, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1038\/sj.emboj.7600808","volume":"24","year":"2005","unstructured":"K. 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Warren, EMBO J.21, 5645 (2002).","journal-title":"EMBO J."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1093\/emboj\/7595006","volume":"22","year":"2003","unstructured":"P. Kannouche et al., EMBO J.22, 1223 (2003).","journal-title":"EMBO J."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1101\/gad.187501","volume":"15","year":"2001","unstructured":"P. Kannouche et al., Genes Dev.15, 158 (2001).","journal-title":"Genes Dev."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.molcel.2005.03.032","volume":"18","year":"2005","unstructured":"E. C. Friedberg, A. R. Lehmann, R. P. Fuchs, Mol. Cell18, 499 (2005).","journal-title":"Mol. Cell"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00991"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S1097-2765(04)00259-X","volume":"14","year":"2004","unstructured":"P. L. 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Chem."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1242\/jcs.00653","volume":"116","year":"2003","unstructured":"G. Maga, U. Hubscher, J. Cell Sci.116, 3051 (2003).","journal-title":"J. Cell Sci."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"R585","DOI":"10.1016\/S0960-9822(03)00518-9","volume":"13","year":"2003","unstructured":"A. Lehmann, Curr. Biol.13, R585 (2003).","journal-title":"Curr. Biol."},{"key":"e_1_3_2_19_2","unstructured":"D. Hoeller et al. Nat. Cell Biol. in press."},{"key":"e_1_3_2_20_2","unstructured":"We thank E. Friedberg and S. Tateishi for help with reagents and W. Mueller-Esterl A. Carr K. Caldecott and members of the Dikic laboratory for critical reading of the manuscript. We also thank J. Wing and I. Konrad for technical assistance. This work was supported by a scholarship from the Ernst Schering Foundation (to M.B.) an MRC Programme Grant and EC Contract MRTN-CT-2003-503618 (to A.R.L.) grants from the Swiss National Science Foundation and Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (to M.P. and G.W.) and grants from the Deutsche Forschungsgemeinschaft (DI 931\/1-1) and Boehringer Ingelheim Fonds (to I.D.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1120615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T21:29:48Z","timestamp":1704835788000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1120615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,16]]},"references-count":19,"journal-issue":{"issue":"5755","published-print":{"date-parts":[[2005,12,16]]}},"alternative-id":["10.1126\/science.1120615"],"URL":"https:\/\/doi.org\/10.1126\/science.1120615","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1021119.358591","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,16]]}}}