{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:23:37Z","timestamp":1791354217179,"version":"4.3.1"},"reference-count":44,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5799","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2006,10,27]]},"abstract":"<jats:p>\n                    Large-conductance calcium- and voltage-activated potassium channels (BK\n                    <jats:sub>Ca<\/jats:sub>\n                    ) are dually activated by membrane depolarization and elevation of cytosolic calcium ions (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ). Under normal cellular conditions, BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channel activation requires Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentrations that typically occur in close proximity to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    sources. We show that BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channels affinity-purified from rat brain are assembled into macromolecular complexes with the voltage-gated calcium channels Cav1.2 (L-type), Cav2.1 (P\/Q-type), and Cav2.2 (N-type). Heterologously expressed BK\n                    <jats:sub>Ca<\/jats:sub>\n                    -Cav complexes reconstitute a functional \u201cCa\n                    <jats:sup>2+<\/jats:sup>\n                    nanodomain\u201d where Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx through the Cav channel activates BK\n                    <jats:sub>Ca<\/jats:sub>\n                    in the physiological voltage range with submillisecond kinetics. Complex formation with distinct Cav channels enables BK\n                    <jats:sub>Ca<\/jats:sub>\n                    -mediated membrane hyperpolarization that controls neuronal firing pattern and release of hormones and transmitters in the central nervous system.\n                  <\/jats:p>","DOI":"10.1126\/science.1132915","type":"journal-article","created":{"date-parts":[[2006,10,26]],"date-time":"2006-10-26T17:11:36Z","timestamp":1161882696000},"page":"615-620","source":"Crossref","is-referenced-by-count":367,"title":["BK\n                    <sub>Ca<\/sub>\n                    -Cav Channel Complexes Mediate Rapid and Localized Ca\n                    <sup>2+<\/sup>\n                    -Activated K\n                    <sup>+<\/sup>\n                    Signaling"],"prefix":"10.1126","volume":"314","author":[{"given":"Henrike","family":"Berkefeld","sequence":"first","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"Sailer","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Bildl","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rg-Oliver","family":"Thumfart","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Eble","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Klugbauer","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Reisinger","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Bischofberger","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Oliver","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Logopharm GmbH, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany."},{"name":"Division of Molecular and Cellular Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1, A-6020 Innsbruck, Austria."},{"name":"Institute of Pharmacology and Toxicology, University of Freiburg, Albertstrasse 25,79104 Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Gu\u0308nther","family":"Knaus","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, 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Jonas for insightful discussions and critical reading of the manuscript and J. Yang for the generous gift of the Cav2.1 clones. This work was supported by grants of the Deutsche Forschungsgemeinschaft to B.F. (Fa 332\/5-1 2 and GRK843)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1132915","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T05:01:26Z","timestamp":1704862886000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1132915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,27]]},"references-count":44,"journal-issue":{"issue":"5799","published-print":{"date-parts":[[2006,10,27]]}},"alternative-id":["10.1126\/science.1132915"],"URL":"https:\/\/doi.org\/10.1126\/science.1132915","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1050402.502310","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,27]]}}}