{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:01:52Z","timestamp":1791295312387,"version":"4.1.0"},"reference-count":30,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5801","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2006,11,10]]},"abstract":"<jats:p>\n            Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that\n            <jats:italic>Shigella<\/jats:italic>\n            movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its \u03b1-tubulin\u2013specific cysteine protease\u2013like activity.\n            <jats:italic>virA<\/jats:italic>\n            mutants did not move within the host cytoplasm and failed to move into adjacent cells.\n          <\/jats:p>","DOI":"10.1126\/science.1133174","type":"journal-article","created":{"date-parts":[[2006,11,9]],"date-time":"2006-11-09T21:53:26Z","timestamp":1163109206000},"page":"985-989","source":"Crossref","is-referenced-by-count":87,"title":["Microtubule-Severing Activity of\n            <i>Shigella<\/i>\n            Is Pivotal for Intercellular Spreading"],"prefix":"10.1126","volume":"314","author":[{"given":"Sei","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan."},{"name":"Department of Basic Medical Sciences, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan."},{"name":"Department of Infectious Control, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan."},{"name":"CREST, Japan Science and Technology Corporation (JST). 4-1-8, Honche, Kawaguchi-shi, Saitama 332-0012, Japan."},{"name":"Laboratory of Bacterial Infection, Kitasato Institute for Life Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Handa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, 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