{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:13:11Z","timestamp":1791317591917,"version":"4.3.1"},"reference-count":19,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5798","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2006,10,20]]},"abstract":"<jats:p>\n                    Diverse bacterial and viral pathogens induce actin polymerization in the cytoplasm of host cells to facilitate infection. Here, we describe a pathogenic mechanism for promoting dynamic actin assembly in the nucleus to enable viral replication. The baculovirus\n                    <jats:italic>Autographa californica<\/jats:italic>\n                    multiple nucleopolyhedrovirus induced nuclear actin polymerization by translocating the host actin-nucleating Arp2\/3 complex into the nucleus, where it was activated by p78\/83, a viral Wiskott-Aldrich syndrome protein (WASP)\u2013like protein. Nuclear actin assembly by p78\/83 and Arp2\/3 complex was essential for viral progeny production. Recompartmentalizing dynamic host actin may represent a conserved mode of pathogenesis and reflect viral manipulation of normal functions of nuclear actin.\n                  <\/jats:p>","DOI":"10.1126\/science.1133348","type":"journal-article","created":{"date-parts":[[2006,10,19]],"date-time":"2006-10-19T16:53:14Z","timestamp":1161276794000},"page":"464-467","source":"Crossref","is-referenced-by-count":150,"title":["Dynamic Nuclear Actin Assembly by Arp2\/3 Complex and a Baculovirus WASP-Like Protein"],"prefix":"10.1126","volume":"314","author":[{"given":"Erin D.","family":"Goley","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Ohkawa","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Mancuso","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey B.","family":"Woodruff","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"D'Alessio","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Zacheus","family":"Cande","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loy E.","family":"Volkman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Welch","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1095"},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1128\/jvi.65.3.1219-1227.1991","volume":"65","year":"1991","unstructured":"C. A. Charlton, L. E. Volkman, J. Virol.65, 1219 (1991).","journal-title":"J. Virol."},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1006\/viro.1993.1585","volume":"197","year":"1993","unstructured":"C. A. Charlton, L. E. Volkman, Virology197, 245 (1993).","journal-title":"Virology"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"12281","DOI":"10.1128\/JVI.76.23.12281-12289.2002","volume":"76","year":"2002","unstructured":"T. Ohkawa, A. R. Rowe, L. E. Volkman, J. Virol.76, 12281 (2002).","journal-title":"J. Virol."},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0042-6822(87)90433-8","volume":"156","year":"1987","unstructured":"L. E. Volkman, P. A. Goldsmith, R. T. Hess, Virology156, 32 (1987).","journal-title":"Virology"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/0042-6822(88)90295-4","volume":"163","year":"1988","unstructured":"L. E. Volkman, Virology163, 547 (1988).","journal-title":"Virology"},{"key":"e_1_3_1_8_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0022-2011(89)90005-0","volume":"53","year":"1989","unstructured":"R. T. Hess, P. A. Goldsmith, L. E. Volkman, J. Invertebr. Pathol.53, 169 (1989).","journal-title":"J. Invertebr. Pathol."},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1083\/jcb.200507101","volume":"172","year":"2006","unstructured":"D. McDonald, G. Carrero, C. Andrin, G. de Vries, M. J. Hendzel, J. Cell Biol.172, 541 (2006).","journal-title":"J. Cell Biol."},{"key":"e_1_3_1_10_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/ncb1357","volume":"8","year":"2006","unstructured":"M. T. Bohnsack, T. Stuven, C. Kuhn, V. C. Cordes, D. Gorlich, Nat. Cell Biol.8, 257 (2006).","journal-title":"Nat. Cell Biol."},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-07-0656"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/352126a0"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.18.040202.112133"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(01)02009-8"},{"key":"e_1_3_1_15_2","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0042-6822(91)90770-C","volume":"185","year":"1991","unstructured":"R. D. Possee et al., Virology185, 229 (1991).","journal-title":"Virology"},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nsb952","volume":"10","year":"2003","unstructured":"S. C. Panchal, D. A. Kaiser, E. Torres, T. D. Pollard, M. K. Rosen, Nat. Struct. Biol.10, 591 (2003).","journal-title":"Nat. Struct. Biol."},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/35050590","volume":"3","year":"2001","unstructured":"J. B. Marchand, D. A. Kaiser, T. D. Pollard, H. N. Higgs, Nat. Cell Biol.3, 76 (2001).","journal-title":"Nat. Cell Biol."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.virusres.2005.09.007","volume":"116","year":"2006","unstructured":"Y. Nie et al., Virus Res.116, 129 (2006).","journal-title":"Virus Res."},{"key":"e_1_3_1_19_2","first-page":"0763","volume":"2","year":"2006","unstructured":"B. Feierbach, S. Piccinotti, M. Bisher, W. Denk, L. W. Enquist, PLoS Pathog.2, 0763 (2006).","journal-title":"PLoS Pathog."},{"key":"e_1_3_1_20_2","unstructured":"We thank R. Tsien and J. Thorner for the mCherry construct M. Roe for T. ni ARPC3\/p21 expressed sequence tags G. Blissard and O. Lung for materials for recombinant bacmid generation J. Vlak for plasmid pCRcass1 L. Guarino for plasmid Eco RI-I\/pUC7 and pp31 antibody M. Goldberg and K. Gunsalus for cofilin antibody J. Washburn for determining WOBpos virus virulence in insects R. Jeng and C. Haglund for preparing dsRNA S. Ruzin and D. Schichnes for microscopy assistance R. Jeng and A. Siripala for comments on the manuscript and the Welch and Volkman labs for helpful discussions. This work was supported by a NSF graduate fellowship (E.D.G.) federal Hatch funds (L.E.V.) NIH National Institute of Allergy and Infectious Diseases grant AI054693 (W.Z.C.) NIH National Institute of General Medical Sciences grant GM59609 (M.D.W.) and grants 2004-35607-14906 (M.D.W.) and 2002-35302-1260 (L.E.V.) from the National Research Initiative of the U.S. Department of Agriculture Cooperative State Research Education Extension Services (CSREES)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1133348","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T05:05:39Z","timestamp":1704863139000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1133348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,20]]},"references-count":19,"journal-issue":{"issue":"5798","published-print":{"date-parts":[[2006,10,20]]}},"alternative-id":["10.1126\/science.1133348"],"URL":"https:\/\/doi.org\/10.1126\/science.1133348","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1047835.498168","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1047835.497851","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,20]]}}}