{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:23:20Z","timestamp":1791048200608,"version":"4.1.0"},"reference-count":29,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5841","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2007,8,24]]},"abstract":"<jats:p>\n                    Histidine kinases, used for environmental sensing by bacterial two-component systems, are involved in regulation of bacterial gene expression, chemotaxis, phototaxis, and virulence. Flavin-containing domains function as light-sensory modules in plant and algal phototropins and in fungal blue-light receptors. We have discovered that the prokaryotes\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    ,\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    ,\n                    <jats:italic>Erythrobacter litoralis<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    contain light-activated histidine kinases that bind a flavin chromophore and undergo photochemistry indicative of cysteinyl-flavin adduct formation. Infection of macrophages by\n                    <jats:italic>B. abortus<\/jats:italic>\n                    was stimulated by light in the wild type but was limited in photochemically inactive and null mutants, indicating that the flavin-containing histidine kinase functions as a photoreceptor regulating\n                    <jats:italic>B. abortus<\/jats:italic>\n                    virulence.\n                  <\/jats:p>","DOI":"10.1126\/science.1144306","type":"journal-article","created":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T16:53:54Z","timestamp":1187888034000},"page":"1090-1093","source":"Crossref","is-referenced-by-count":242,"title":["Blue-Light-Activated Histidine Kinases: Two-Component Sensors in Bacteria"],"prefix":"10.1126","volume":"317","author":[{"given":"Trevor E.","family":"Swartz","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong-Seung","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus A.","family":"Frederickson","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gasto\u0301n","family":"Paris","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gireesh","family":"Rajashekara","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Gun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Beth","family":"Mudgett","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary A.","family":"Splitter","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos 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A.","family":"Bogomolni","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA."},{"name":"Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA, USA."},{"name":"Fundacio\u0301n Instituto Leloir, Instituto de Investigaciones Bioqui\u0301micas Buenos Aires\u2013Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IIBBA-CONICET), Buenos Aires, Argentina."},{"name":"Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA."},{"name":"Department of Biological Sciences, Stanford University, Stanford, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","first-page":"323","year":"2005","unstructured":"S. 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Ng for experimental advice and to S. Zulman for critical reading of the manuscript. Work in the authors' laboratories was supported by NSF grants MCB 0444390 (R.A.B.) and MCB 0091384 and 0444504 (W.R. B.); NIH 1.U54-AI-057153 Binational Agricultural Research and Development Fund US-3829-06 and USDA 35204-14856 (G.S.); and NIH RO1-GM068886 (M.B.M.) ANPCyT PICT2004-20408 (R.A.U.) and PICT2005-38272 (D.J.C.). The research of F.A.G. was supported in part by an International Research Scholars' grant from the Howard Hughes Medical Institute and by Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica\u2013Proyectos de Invest\u00edgaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (ANPCYT-PICT2004-21193). We thank S. Giovannoni and his laboratory and the J. Craig Venter Institute (JCVI) for the E. litoralis HTCC2594 genome sequence which was determined and deposited in GenBank (CP000157) with support from the Gordon and Betty Moore Foundation. We are indebted to S. Kravitz and S. Ferriera of JCVI and J. Kitner J. Tripp S. Givan and K. Vergin of Oregon State University for their work on the E. litoralis genome sequence and for the DNA used to clone the E. litoralis LOV domains."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1144306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:14:57Z","timestamp":1704856497000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1144306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,24]]},"references-count":29,"journal-issue":{"issue":"5841","published-print":{"date-parts":[[2007,8,24]]}},"alternative-id":["10.1126\/science.1144306"],"URL":"https:\/\/doi.org\/10.1126\/science.1144306","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1090543.543817","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1090543.546080","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1090543.545024","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,24]]}}}