{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:47:17Z","timestamp":1791341237053,"version":"4.3.1"},"reference-count":31,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5845","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2007,9,21]]},"abstract":"<jats:p>\n                    Mammalian spermatogenesis produces numerous sperm for a long period based on a highly potent stem cell system, which relies on a special microenvironment, or niche, that has not yet been identified. In this study, using time-lapse imaging of green fluorescent protein\u2013labeled undifferentiated spermatogonia (A\n                    <jats:sub>undiff<\/jats:sub>\n                    ) and three-dimensional reconstitution, we revealed a biased localization of A\n                    <jats:sub>undiff<\/jats:sub>\n                    to the vascular network and accompanying Leydig and other interstitial cells, in intact testes. Differentiating spermatogonia left these niche regions and dispersed throughout the basal compartment of the seminiferous epithelium. Moreover, rearrangement of A\n                    <jats:sub>undiff<\/jats:sub>\n                    accompanied the vasculature alteration. We propose that the mammalian germline niche is established as a consequence of vasculature pattern formation. This is different from what is observed in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    or\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    , which display developmentally specified niche structures within polarized gonads.\n                  <\/jats:p>","DOI":"10.1126\/science.1144885","type":"journal-article","created":{"date-parts":[[2007,9,6]],"date-time":"2007-09-06T21:03:33Z","timestamp":1189112613000},"page":"1722-1726","source":"Crossref","is-referenced-by-count":439,"title":["A Vasculature-Associated Niche for Undifferentiated Spermatogonia in the Mouse Testis"],"prefix":"10.1126","volume":"317","author":[{"given":"Shosei","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamiko","family":"Sukeno","sequence":"additional","affiliation":[{"name":"Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yo-ichi","family":"Nabeshima","sequence":"additional","affiliation":[{"name":"Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35102160"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04957"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1532\/IJH97.05097"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1532\/IJH97.05088"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111158198"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.11.2775"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03894"},{"key":"e_1_3_1_9_2","first-page":"238","year":"1993","unstructured":"B. T. Hilton, T. T. Turner, in Cell and Molecular Biology of the Testis, C. Desjardins, L. L. Ewing, Eds. (Oxford Univ. Press, New York, 1993), pp. 238\u2013265.","journal-title":"Cell and Molecular Biology of the Testis"},{"key":"e_1_3_1_10_2","unstructured":"L. Russell R. Ettlin A. Sinha Hikim E. Clegg Histological and Histopathological Evaluation of the Testis (Cache River Press Clearwater FL 1990)."},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)43045-1"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.01.002"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1952.tb26576.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1002\/j.1939-4640.2000.tb03408.x","volume":"21","year":"2000","unstructured":"D. G. de Rooij, L. D. Russell, J. Androl.21, 776 (2000).","journal-title":"J. Androl."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(93)90159-D"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1210347"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.15.8346"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00111-8"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.4.1170"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.4.1179"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.01.036"},{"key":"e_1_3_1_22_2","unstructured":"Materials and methods are available as supporting material on Science Online."},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02316"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.11.005"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.10.016"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001002)425:4<479::AID-CNE2>3.0.CO;2-3"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.11.020"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409107"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0578"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001080106"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/288551a0"},{"key":"e_1_3_1_32_2","unstructured":"We thank D. de Rooij for providing instructions regarding seminiferous epithelial stage determination; T. Obata for preparing sections; T. Fujimori and K. Kato for their suggestions about imaging; N. Yoshida T. Nakagawa and R. Sugimoto for critical reading of the manuscript. We also thank members of the Institute of Laboratory Animals Graduate School of Medicine Kyoto University for animal care. This work was partly supported by Grants-in-Aid for Scientific Research from the Ministry of Education Culture Sports Science and Technology Japan. The initial part of this study was supported by the PRESTO program (Recognition and Formation) Japan Science and Technology Agency."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1144885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T04:32:24Z","timestamp":1704861144000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1144885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,21]]},"references-count":31,"journal-issue":{"issue":"5845","published-print":{"date-parts":[[2007,9,21]]}},"alternative-id":["10.1126\/science.1144885"],"URL":"https:\/\/doi.org\/10.1126\/science.1144885","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1091060.547695","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1091060.545360","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1091060.544443","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,21]]}}}