{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:58:52Z","timestamp":1791363532732,"version":"4.3.1"},"reference-count":33,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5888","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2008,7,25]]},"abstract":"<jats:p>\n                    Biological formation and consumption of molecular hydrogen (H\n                    <jats:sub>2<\/jats:sub>\n                    ) are catalyzed by hydrogenases, of which three phylogenetically unrelated types are known: [NiFe]-hydrogenases, [FeFe]-hydrogenases, and [Fe]-hydrogenase. We present a crystal structure of [Fe]-hydrogenase at 1.75 angstrom resolution, showing a mononuclear iron coordinated by the sulfur of cysteine 176, two carbon monoxide (CO) molecules, and the sp\n                    <jats:sup>2<\/jats:sup>\n                    -hybridized nitrogen of a 2-pyridinol compound with back-bonding properties similar to those of cyanide. The three-dimensional arrangement of the ligands is similar to that of thiolate, CO, and cyanide ligated to the low-spin iron in binuclear [NiFe]- and [FeFe]-hydrogenases, although the enzymes have evolved independently and the CO and cyanide ligands are not found in any other metalloenzyme. The related iron ligation pattern of hydrogenases exemplifies convergent evolution and presumably plays an essential role in H\n                    <jats:sub>2<\/jats:sub>\n                    activation. This finding may stimulate the ongoing synthesis of catalysts that could substitute for platinum in applications such as fuel cells.\n                  <\/jats:p>","DOI":"10.1126\/science.1158978","type":"journal-article","created":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T17:24:54Z","timestamp":1216920294000},"page":"572-575","source":"Crossref","is-referenced-by-count":591,"title":["The Crystal Structure of [Fe]-Hydrogenase Reveals the Geometry of the Active Site"],"prefix":"10.1126","volume":"321","author":[{"given":"Seigo","family":"Shima","sequence":"first","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Pilak","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Vogt","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schick","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco S.","family":"Stagni","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Meyer-Klaucke","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eberhard","family":"Warkentin","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf K.","family":"Thauer","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Ermler","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Terrestrische Mikrobiologie and Laboratorium fu\u0308r Mikrobiologie, Fachbereich Biologie, Philipps-Universita\u0308t Marburg, Karl-von-Frisch-Stra\u00dfe, D-35043 Marburg, Germany."},{"name":"EMBL Hamburg, Notkestr. 85, D-22603 Hamburg, Germany."},{"name":"Max-Planck-Institut fu\u0308r Biophysik, Max-von-Laue-Stra\u00dfe 3, D-60438 Frankfurt\/Main, 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supporting material on Science Online."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0010-8545(94)01117-T"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00214-007-0321-7"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(06)51001-X"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-007-0302-2"},{"key":"e_1_3_2_34_2","unstructured":"Supported by the Max Planck Society the Fonds der Chemischen Industrie and the Bundesministerium f\u00fcr Bildung und Forschung (BioH 2 project). We thank H. Michel for continuous support and the staff of beamline PXII at the Swiss Light Source for help during data collection. Coordinates of [Fe]-hydrogenase and [Fe]-hydrogenase complexed with cyanide have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank under accession codes 3DAG and 3DAF."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1158978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:20:40Z","timestamp":1704856840000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1158978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,25]]},"references-count":33,"journal-issue":{"issue":"5888","published-print":{"date-parts":[[2008,7,25]]}},"alternative-id":["10.1126\/science.1158978"],"URL":"https:\/\/doi.org\/10.1126\/science.1158978","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1118750.574982","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1118750.575943","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1118750.574822","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,25]]}}}