{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:49:30Z","timestamp":1792511370622,"version":"4.5.0"},"reference-count":31,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5885","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2008,7,4]]},"abstract":"<jats:p>\n                    In neurons, individual dendritic spines isolate\n                    <jats:italic>N<\/jats:italic>\n                    -methyl-\n                    <jats:sc>d<\/jats:sc>\n                    -aspartate (NMDA) receptor\u2013mediated calcium ion (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent Ras activation in single spines that decayed in \u223c5 minutes. Ras activity spread over \u223c10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.\n                  <\/jats:p>","DOI":"10.1126\/science.1159675","type":"journal-article","created":{"date-parts":[[2008,6,12]],"date-time":"2008-06-12T22:18:04Z","timestamp":1213309084000},"page":"136-140","source":"Crossref","is-referenced-by-count":398,"title":["The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine"],"prefix":"10.1126","volume":"321","author":[{"given":"Christopher D.","family":"Harvey","sequence":"first","affiliation":[{"name":"Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA."},{"name":"Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA."},{"name":"Neurobiology Department, Duke University Medical Center, Durham, NC 27710, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryohei","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA."},{"name":"Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA."},{"name":"Neurobiology Department, Duke University Medical Center, Durham, NC 27710, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haining","family":"Zhong","sequence":"additional","affiliation":[{"name":"Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA."},{"name":"Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA."},{"name":"Neurobiology Department, Duke University Medical Center, Durham, NC 27710, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karel","family":"Svoboda","sequence":"additional","affiliation":[{"name":"Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA."},{"name":"Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA."},{"name":"Neurobiology Department, Duke University Medical Center, Durham, NC 27710, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.64.081501.160008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5262.716"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114816"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/354073a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/375682a0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00573-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1685"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.04.031"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600151"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.10.5773"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00897-8"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.09.012"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02617"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040271"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040370"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06416"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1635"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35082594"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105654"},{"key":"e_1_3_2_21_2","first-page":"379","year":"1992","unstructured":"D. W. Piston, D. R. Sandison, W. W. Webb, in Time-Resolved Laser Spectroscopy in Biochemistry III, J. R. Lakowicz, Ed. (SPIE, Bellingham, WA, 1992), pp. 379\u2013389.","journal-title":"Time-Resolved Laser Spectroscopy in Biochemistry III"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1586704"},{"key":"e_1_3_2_23_2","unstructured":"Supporting online material is available on Science Online."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5066-03.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3918-05.2006"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.11.051"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074952"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/35078615"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200202009"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74139-9"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06725"},{"key":"e_1_3_2_32_2","unstructured":"We thank N. Ghitani B. Burbach C. Zhang B. Shields and H. White for technical assistance; N. Gray for help with immunostaining; L. van Aelst for helpful discussions; and J. Dudman for comments on the manuscript. This work was supported by the Howard Hughes Medical Institute NIH a David and Fanny Luke Fellowship (C.D.H.) Burroughs Wellcome Fund (R.Y.) Dana Foundation (R.Y.) National Alliance for Autism Research (R.Y.) National Institute of Mental Health (R.Y.) and National Alliance for Research on Schizophrenia and Depression (H.Z.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1159675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T04:04:10Z","timestamp":1704859450000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1159675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,4]]},"references-count":31,"journal-issue":{"issue":"5885","published-print":{"date-parts":[[2008,7,4]]}},"alternative-id":["10.1126\/science.1159675"],"URL":"https:\/\/doi.org\/10.1126\/science.1159675","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1141896.599005","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,4]]}}}