{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:46:36Z","timestamp":1791978396748,"version":"4.4.0"},"reference-count":26,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5913","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2009,1,23]]},"abstract":"<jats:p>\n                    Sphingosine-1-phosphate (S1P) is a secreted lipid mediator that functions in vascular development; however, it remains unclear how S1P secretion is regulated during embryogenesis. We identified a zebrafish mutant,\n                    <jats:italic>ko157<\/jats:italic>\n                    , that displays cardia bifida (two hearts) resembling that in the\n                    <jats:italic>S1P receptor-2<\/jats:italic>\n                    mutant. A migration defect of myocardial precursors in the\n                    <jats:italic>ko157<\/jats:italic>\n                    mutant is due to a mutation in a multipass transmembrane protein, Spns2, and can be rescued by S1P injection. We show that the export of S1P from cells requires Spns2.\n                    <jats:italic>spns2<\/jats:italic>\n                    is expressed in the extraembryonic tissue yolk syncytial layer (YSL), and the introduction of\n                    <jats:italic>spns2<\/jats:italic>\n                    mRNA in the YSL restored the cardiac defect in the\n                    <jats:italic>ko157<\/jats:italic>\n                    mutant. Thus, Spns2 in the YSL functions as a S1P transporter in S1P secretion, thereby regulating myocardial precursor migration.\n                  <\/jats:p>","DOI":"10.1126\/science.1167449","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T20:24:17Z","timestamp":1229113457000},"page":"524-527","source":"Crossref","is-referenced-by-count":387,"title":["The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors"],"prefix":"10.1126","volume":"323","author":[{"given":"Atsuo","family":"Kawahara","sequence":"first","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Nishi","sequence":"additional","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hisano","sequence":"additional","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihito","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Structural Analysis, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan."},{"name":"HMRO, Kyoto University Faculty of Medicine, Yoshida, Sakyo-Ku, Kyoto 606-8501, Japan."},{"name":"Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibarakishi, Osaka 567-0047, Japan."},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Suita-shi, Osaka 565-0871, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35047564"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2006.12.010"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)02075-8"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35018092"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1552"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-6618(03)00046-X"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1103"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2329"},{"issue":"1","key":"e_1_3_1_10_2","first-page":"S77","volume":"4","year":"2007","unstructured":"T. Matsuiet al., Nat. Clin. Pract. Cardiovasc. Med.4 (suppl. 1), S77 (2007).","journal-title":"Nat. Clin. Pract. Cardiovasc. Med."},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.11.3775-3788.2001"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10060"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1087619"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M500468-JLR200"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603734103"},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1111\/j.1471-4159.2007.04958.x","volume":"103","year":"2007","unstructured":"K. Satoet al., J. Neurochem.103, 2610 (2007).","journal-title":"J. Neurochem."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103264200"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2007.08.001"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.892301"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.196901"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02581"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/35001596"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/90038"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200113468"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.3.1921"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.02.025"},{"key":"e_1_3_1_27_2","unstructured":"We thank M. Sone and M. Minamimoto for technical assistance; S. Endo and R. Hanaoka for the mutagenesis screening; T. Kitaguchi for the establishment of cmlc2:mRFP; M. Masuda Y. Igarashi A. Kihara S. Mitsutake K. Shioya I. B. Dawid and Y. Kaziro for support and suggestions; M. Hibi J. S. Gutkind and M. Tsang for critical reading and valuable suggestions; D. Y. Stainier for discussions and sharing of unpublished data; and H. Okamoto K. Kawakami Y. Kikuchi H. Yanagisawa D. Yamamoto R. Y. Tsien and H.-J. Tsai for fish and reagents. Supported by grants from the Ministry of Education Culture Sports Science and Technology of Japan; the National BioResource Project of Japan; the Japan Society for the Promotion of Science; the Program for the Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation; the Ministry of Health Labor and Welfare of Japan; and the Takeda Science Foundation. The sequences of zebrafish spns2 human Spns2 and mouse Spns2 have been deposited in the DNA Data Bank of Japan [accession numbers: AB441164 (zebrafish) AB441165 (human) AB441166 (mouse)]. The authors are filing a patent based on the results reported in this paper."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1167449","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T03:47:17Z","timestamp":1704858437000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1167449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,23]]},"references-count":26,"journal-issue":{"issue":"5913","published-print":{"date-parts":[[2009,1,23]]}},"alternative-id":["10.1126\/science.1167449"],"URL":"https:\/\/doi.org\/10.1126\/science.1167449","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,23]]}}}