{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:42:02Z","timestamp":1791322922993,"version":"4.3.1"},"reference-count":26,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5936","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2009,7,3]]},"abstract":"<jats:title>Breaking the Barrier<\/jats:title>\n                  <jats:p>\n                    Being able to deliver drugs into the brain to treat degenerative diseases such as Alzheimer's or Parkinson's requires the ability to traverse the blood-brain barrier (BBB). Understanding the formation of the very specific adherent junctions (AJ) and tight junctions present at the BBB cell junctions is a prerequisite to the design of such therapeutics. However, diminishing the expression of any one component involved in the formation of these intercellular junctions destroys them.\n                    <jats:bold>\n                      Coureuil\n                      <jats:italic>et al.<\/jats:italic>\n                    <\/jats:bold>\n                    (p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" page=\"83\" related-article-type=\"in-this-issue\" vol=\"325\" xlink:href=\"10.1126\/science.1173196\">83<\/jats:related-article>\n                    , published online 11 June) exploited the specific recruitment of AJ proteins by\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    to dissect this process. Adhesion of the bacteria to human brain endothelial cells recruited the polarity complex Par3\/Par6\/PKC\u03b6 required for the establishment of eukaryotic cell polarity and the formation of intercellular junctions. The bacterial recruitment of the polarity complex depleted junctional proteins at the cell-cell interface opening the intercellular junctions at the brainendothelial interface.\n                  <\/jats:p>","DOI":"10.1126\/science.1173196","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T22:32:25Z","timestamp":1244759545000},"page":"83-87","source":"Crossref","is-referenced-by-count":217,"title":["Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium"],"prefix":"10.1126","volume":"325","author":[{"given":"Mathieu","family":"Coureuil","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillain","family":"Mikaty","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Miller","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS (UMR 8104), 75015 Paris, France."},{"name":"INSERM, U567, 75014 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"L\u00e9cuyer","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."},{"name":"AP-HP, H\u00f4pital Necker-Enfants Malades, Paris F-75015, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Bernard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bourdoulous","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS (UMR 8104), 75015 Paris, France."},{"name":"INSERM, U567, 75014 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Dum\u00e9nil","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9-Marc","family":"M\u00e8ge","sequence":"additional","affiliation":[{"name":"INSERM UMR-S 839, Universit\u00e9 Pierre et Marie Curie-Paris6, Institut du Fer \u00e0 Moulin, 75005 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babette B.","family":"Weksler","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College, New York, NY 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio A.","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, The Open University, Walton Hall, Milton Keynes MK7 6AA, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Olivier","family":"Couraud","sequence":"additional","affiliation":[{"name":"Institut Cochin, Universit\u00e9 Paris Descartes, CNRS (UMR 8104), 75015 Paris, France."},{"name":"INSERM, U567, 75014 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Nassif","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, INSERM (U-570), 75015 Paris, France."},{"name":"AP-HP, H\u00f4pital Necker-Enfants Malades, Paris F-75015, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.1.144-166.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/514124"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060482"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00807.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01615.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/373357a0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4841857.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3072-3082.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/35024105"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.160938"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01459.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000314"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.6.1231"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3458fje"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.1.149"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.2001.00453.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb923"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81559-7"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/35019573"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.020693"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200412172"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02898"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03470"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702731104"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200106148"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-967137"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1173196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T04:41:48Z","timestamp":1704861708000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1173196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,3]]},"references-count":26,"journal-issue":{"issue":"5936","published-print":{"date-parts":[[2009,7,3]]}},"alternative-id":["10.1126\/science.1173196"],"URL":"https:\/\/doi.org\/10.1126\/science.1173196","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1162428.623422","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1162428.622945","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,3]]}}}