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Diffusion of Ca\n            <jats:sup>2+<\/jats:sup>\n            through the cytosol is so constrained that a rise in cytoplasmic Ca\n            <jats:sup>2+<\/jats:sup>\n            concentration produced by local Ca\n            <jats:sup>2+<\/jats:sup>\n            entry through cell membrane or by local Ca\n            <jats:sup>2+<\/jats:sup>\n            injection is confined to the immediate vicinity of these sites. The diffusion constraints are lifted by treatment with cyanide or ruthenium red. Thus, energized calcium sequestering, probably by mitochondria, is the dominant factor in the constraints. In cell regions where the sequestering machinery is sufficiently dense, different Ca\n            <jats:sup>2+<\/jats:sup>\n            message functions inside a cell may be effectively segregated, permitting private-line intracellular communication.\n          <\/jats:p>","DOI":"10.1126\/science.1198106","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T15:11:22Z","timestamp":1160061082000},"page":"1204-1206","source":"Crossref","is-referenced-by-count":144,"title":["Calcium Ion Distribution in Cytoplasm Visualized by Aequorin: Diffusion in Cytosol Restricted by Energized Sequestering"],"prefix":"10.1126","volume":"190","author":[{"given":"Birgit","family":"Rose","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33152"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner R.","family":"Loewenstein","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33152"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1113\/jphysiol.1970.sp009158","volume":"209","year":"1970","unstructured":"ASHLEY, C.C., ON RELATIONSHIPS BETWEEN MEMBRANE POTENTIAL, CALCIUM TRANSIENT AND TENSION IN SINGLE BARNACLE MUSCLE FIBRES, JOURNAL OF PHYSIOLOGY-LONDON 209: 105 (1970).","journal-title":"JOURNAL OF PHYSIOLOGY-LONDON"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0005-2728(69)90042-5","volume":"189","year":"1969","unstructured":"AZZI, A, ENERGIZED STATE OF MITOCHONDRIA - 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