{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:55:45Z","timestamp":1791338145811,"version":"4.3.1"},"reference-count":38,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6098","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2012,8,31]]},"abstract":"<jats:title>Ocean Methane<\/jats:title>\n                  <jats:p>\n                    Is methylphosphonate a natural product? If it is, then its catabolism by microrganisms seeking phosphorus might explain the ubiquity of methane in the oceans. Anaerobic Archaea, so far, are the only significant source of methane known. Thus,\n                    <jats:bold>\n                      Metcalf\n                      <jats:italic>et al.<\/jats:italic>\n                    <\/jats:bold>\n                    (p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" page=\"1104\" related-article-type=\"in-this-issue\" vol=\"337\" xlink:href=\"10.1126\/science.1219875\">1104<\/jats:related-article>\n                    ) looked for evidence of methylphosphonate biochemistry in the widespread archaeon\n                    <jats:italic>Nitrosopumilus maritimus.<\/jats:italic>\n                    The organism can synthesize C-P bonds using phosphoenolpyruvate mutase. By using its gene,\n                    <jats:italic>ppm<\/jats:italic>\n                    , as a marker, a possible phosphonate biosynthesis gene cluster was identified, and adjacent to this a gene for a cupin was found. Together with Fe(II) and O\n                    <jats:sub>2<\/jats:sub>\n                    the cupin catalyzed the production of methylphosphonate and formate. Homologs of the cupin gene are found in a wide range of common marine microbes, including\n                    <jats:italic>Pelagibacter ubique<\/jats:italic>\n                    and\n                    <jats:italic>Prochlorococcus.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1126\/science.1219875","type":"journal-article","created":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T14:17:34Z","timestamp":1346336254000},"page":"1104-1107","source":"Crossref","is-referenced-by-count":286,"title":["Synthesis of Methylphosphonic Acid by Marine Microbes: A Source for Methane in the Aerobic Ocean"],"prefix":"10.1126","volume":"337","author":[{"given":"William W.","family":"Metcalf","sequence":"first","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Microbiology, University of Illinois, 601 South Goodwin Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin M.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Cicchillo","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana\u2013Champaign, 600 South Matthews Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangtao","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana\u2013Champaign, 600 South Matthews Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarath Chandra","family":"Janga","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather A.","family":"Cooke","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana\u2013Champaign, 600 South Matthews Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin T.","family":"Circello","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Microbiology, University of Illinois, 601 South Goodwin Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley S.","family":"Evans","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willm","family":"Martens-Habbena","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Washington, 302 More Hall, Box 352700, Seattle, WA 98195\u20132700, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Stahl","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Washington, 302 More Hall, Box 352700, Seattle, WA 98195\u20132700, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfred A.","family":"van der Donk","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois, 1206 West Gregory, Urbana, IL 61801, USA."},{"name":"Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana\u2013Champaign, 600 South Matthews Avenue, Urbana, IL 61801, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","unstructured":"J. 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