{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:17:03Z","timestamp":1792675023807,"version":"4.5.0"},"reference-count":42,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6190","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2014,6,20]]},"abstract":"<jats:title>Breaking through the epithelial barrier<\/jats:title>\n          <jats:p>\n            Botulinum neurotoxin (BoNT) poisons its host when it crosses the intestinal epithelial barrier. To help it cross this barrier, the toxin forms a large complex with three bacterial proteins called hemagglutinins (HAs). To find out what happens when this complex binds to a cell-adhesion protein called E-cadherin, Lee\n            <jats:italic>et al.<\/jats:italic>\n            crystallized the bound complex and protein. Toxin binding disrupted the way E-cadherin maintains the epithelial barrier. When the researchers prevented the toxin complex from binding to E-cadherin, mice were protected from the toxin's deadly effects.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Science<\/jats:italic>\n            , this issue p.\n            <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6190\" page=\"1405\" related-article-type=\"in-this-issue\" vol=\"344\" xlink:href=\"10.1126\/science.1253823\">1405<\/jats:related-article>\n          <\/jats:p>","DOI":"10.1126\/science.1253823","type":"journal-article","created":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T03:36:24Z","timestamp":1403235384000},"page":"1405-1410","source":"Crossref","is-referenced-by-count":117,"title":["Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex"],"prefix":"10.1126","volume":"344","author":[{"given":"Kwangkook","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofen","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Division of Neuroscience, New England Primate Research Center, Southborough, MA 01772, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenyan","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Magdalena","family":"Kruel","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin B.","family":"Dorner","sequence":"additional","affiliation":[{"name":"Centre for Biological Threats and Special Pathogens\u2013Biological Toxins (ZBS3), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay","family":"Perry","sequence":"additional","affiliation":[{"name":"Northeastern Collaborative Access Team (NE-CAT) and Department of Chemistry and Chemical Biology, Cornell University, Building 436E, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Rummel","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Toxikologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Division of Neuroscience, New England Primate Research Center, Southborough, MA 01772, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongsheng","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1997.00313.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-33570-9_1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1214270"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.16.1.107-109.1977"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/iai.43.2.487-490.1984","article-title":"Oral toxicities of Clostridium botulinum type A and B toxins from different strains","volume":"43","author":"Ohishi I.","year":"1984","unstructured":"Ohishi I., Oral toxicities of Clostridium botulinum type A and B toxins from different strains. Infect. Immun. 43, 487\u2013490 (1984).6693168","journal-title":"Infect. Immun."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(82)90061-4"},{"key":"e_1_3_2_8_2","first-page":"355","article-title":"The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption","volume":"10","author":"Matsumura T.","year":"2008","unstructured":"Matsumura T., Jin Y., Kabumoto Y., Takegahara Y., Oguma K., Lencer W. I., Fujinaga Y., The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption. Cell. Microbiol. 10, 355\u2013364 (2008).17868282","journal-title":"Cell. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/j.1574-695X.2011.00779.x","volume":"61","author":"Ito H.","year":"2011","unstructured":"Ito H., et al.., FEMS Immunol. Med. Microbiol. 61, 323\u2013331 (2011).","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003690"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.04.018"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.521179"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200910119"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a002899"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.03.004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2010.11.016"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003053"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.08.011"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071559"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0201402"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9705624"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2051"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1784"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01136-4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201009141"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01882-09"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-33570-9_3"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.066845"},{"key":"e_1_3_2_29_2","first-page":"375","article-title":"Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers","volume":"10","author":"Couesnon A.","year":"2008","unstructured":"Couesnon A., Pereira Y., Popoff M. R., Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers. Cell. Microbiol. 10, 375\u2013387 (2008).17900298","journal-title":"Cell. Microbiol."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08196.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2013.06.010"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910048675"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444903008126"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/355472a0"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.23.11834-11850.2001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/15.4.305"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6689"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1128\/iai.64.5.1589-1594.1996","article-title":"Molecular composition of Clostridium botulinum type A progenitor toxins","volume":"64","author":"Inoue K.","year":"1996","unstructured":"Inoue K., Fujinaga Y., Watanabe T., Ohyama T., Takeshi K., Moriishi K., Nakajima H., Inoue K., Oguma K., Molecular composition of Clostridium botulinum type A progenitor toxins. Infect. Immun. 64, 1589\u20131594 (1996).8613365","journal-title":"Infect. Immun."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1253823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T20:41:04Z","timestamp":1704919264000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1253823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,20]]},"references-count":42,"journal-issue":{"issue":"6190","published-print":{"date-parts":[[2014,6,20]]}},"alternative-id":["10.1126\/science.1253823"],"URL":"https:\/\/doi.org\/10.1126\/science.1253823","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,20]]}}}