{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:49:33Z","timestamp":1791276573186,"version":"4.1.0"},"reference-count":51,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6203","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2014,9,19]]},"abstract":"<jats:title>A foreign-DNA\u2013destroying machine<\/jats:title>\n                  <jats:p>\n                    Bacteria have an adaptive immune system, called CRISPR, that identifies invading viruses through their DNA or RNA sequences and cuts them up (see the Perspective by Zhang and Sontheimer). Jackson\n                    <jats:italic>et al.<\/jats:italic>\n                    and Mulepati\n                    <jats:italic>et al.<\/jats:italic>\n                    have determined the structure of the large protein complex, called Cascade, that targets the invading nucleic acids and does the cutting. The seahorse-shaped structure reveals how the 11 subcomponents of Cascade assemble into the final protein complex. The structure also shows how Cascade presents the short CRISPR-derived RNAs so that they can bind and target foreign DNA.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Science<\/jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6203\" page=\"1473\" related-article-type=\"in-this-issue\" vol=\"345\" xlink:href=\"10.1126\/science.1256328\">1473<\/jats:related-article>\n                    and p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6203\" page=\"1479\" related-article-type=\"in-this-issue\" vol=\"345\" xlink:href=\"10.1126\/science.1256996\">1479<\/jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6203\" page=\"1452\" related-article-type=\"in-this-issue\" vol=\"345\" xlink:href=\"10.1126\/science.1260026\">1452<\/jats:related-article>\n                  <\/jats:p>","DOI":"10.1126\/science.1256328","type":"journal-article","created":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T03:07:20Z","timestamp":1407467240000},"page":"1473-1479","source":"Crossref","is-referenced-by-count":260,"title":["Crystal structure of the CRISPR RNA\u2013guided surveillance complex from\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1126","volume":"345","author":[{"given":"Ryan N.","family":"Jackson","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Golden","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul B. G.","family":"van Erp","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edze R.","family":"Westra","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stan J. J.","family":"Brouns","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"van der Oost","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Terwilliger","sequence":"additional","affiliation":[{"name":"Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy J.","family":"Read","sequence":"additional","affiliation":[{"name":"Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, 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