{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:20:09Z","timestamp":1762176009778},"reference-count":12,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3303","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1958,4,18]]},"abstract":"<jats:p>Techniques of column chromatography with cellulose ion exchangers have been successfully applied to mammalian viruses and rickettsiae. Recovery of virus is excellent, and appreciable purification in terms of phosphorus and protein removal has been demonstrated.<\/jats:p>\n          <jats:p>Elution characteristics of poliovirus (types 1, 2, and 3), and Coxsackie A9 virus are similar, whereas those of ECHO-13 and Colorado tick fever differ from them as well as from each other.<\/jats:p>\n          <jats:p>\n            Elution diagrams of preparations of ECHO-13 and polio 2 viruses grown on P\n            <jats:sup>32<\/jats:sup>\n            -labeled tissue cultures show a high degree of correlation between the distribution of titratable virus and the distribution of radioactivity.\n          <\/jats:p>\n          <jats:p>A single adsorption and elution of Q fever or epidemic typhus fever rickettsiae results in a striking degree of purification, as demonstrated by electron micrographs.<\/jats:p>\n          <jats:p>The chromatographic behavior of the animal viruses and rickettsiae appears to depend more upon the chemical nature of the surfaces of these infectious agents than upon their size.<\/jats:p>\n          <jats:p>\n            The chromatographic procedure described may prove useful in the preparation of purified, P\n            <jats:sup>32<\/jats:sup>\n            -labeled, fully infectious animal viruses for further fundamental research. It may also prove useful for the removal of unwanted host materials in the preparation of vaccines.\n          <\/jats:p>","DOI":"10.1126\/science.127.3303.859","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T07:41:34Z","timestamp":1160034094000},"page":"859-863","source":"Crossref","is-referenced-by-count":55,"title":["Mammalian Viruses and Rickettsiae"],"prefix":"10.1126","volume":"127","author":[{"given":"Bill H.","family":"Hoyer","sequence":"first","affiliation":[{"name":"Staff member of the Rocky Mountain Laboratory, Hamilton, Mont."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellis T.","family":"Bolton","sequence":"additional","affiliation":[{"name":"Staff member of the Department of Terrestrial Magnetism, Carnegie Institution of Washington, Washington, D.C."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Ormsbee","sequence":"additional","affiliation":[{"name":"Staff member of the Rocky Mountain Laboratory, Hamilton, Mont."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"LeBouvier","sequence":"additional","affiliation":[{"name":"James Hudson Brown research fellow of the Yale University School of Medicine, New Haven, Conn."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel B.","family":"Ritter","sequence":"additional","affiliation":[{"name":"Staff member of the Rocky Mountain Laboratory, Hamilton, Mont."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl L.","family":"Larson","sequence":"additional","affiliation":[{"name":"Staff member of the Rocky Mountain Laboratory, Hamilton, Mont."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"730","DOI":"10.3181\/00379727-92-22595","volume":"92","year":"1956","unstructured":"BERMAN, L, 8 CULTURE STRAINS (DETROIT) OF HUMAN EPITHELIAL-LIKE CELLS, PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 92: 730 (1956).","journal-title":"PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/S0021-9258(19)51000-6","volume":"184","year":"1950","unstructured":"BOVARNICK, M.R., OXIDATION AND TRANSAMINATION OF GLUTAMATE BY TYPHUS RICKETTSIAE, JOURNAL OF BIOLOGICAL CHEMISTRY 184: 661 (1950).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1038\/178767a0","volume":"178","year":"1956","unstructured":"COMMONER, B, RECONSTITUTION OF TOBACCO MOSAIC VIRUS COMPONENTS, NATURE 178: 767 (1956).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/0042-6822(57)90057-0","volume":"4","year":"1957","unstructured":"CREASER, E.H., THE PURIFICATION AND CHROMATOGRAPHY OF BACTERIOPHAGES ON ANION-EXCHANGE CELLULOSE, VIROLOGY 4: 200 (1957).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1084\/jem.102.5.595","volume":"102","year":"1955","unstructured":"EAGLE, H, THE MINIMUM VITAMIN REQUIREMENTS OF THE L-CELLS AND HELA CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE, JOURNAL OF EXPERIMENTAL MEDICINE 102: 595 (1955).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_6_1","unstructured":"HOYER B.H. UNPUBLISHED."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1021\/ja01585a016","volume":"78","year":"1956","unstructured":"PETERSON, E.A., CHROMATOGRAPHY OF PROTEINS .1. CELLULOSE ION-EXCHANGE ADSORBENTS, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 78: 751 (1956).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_9_1","first-page":"493","volume":"27","year":"1938","unstructured":"REED, L.J., AMERICAN JOURNAL OF HYGIENE 27: 493 (1938).","journal-title":"AMERICAN JOURNAL OF HYGIENE"},{"key":"e_1_2_1_10_1","first-page":"113","volume":"29","year":"1953","unstructured":"SCHERER, W.F., THE UTILIZATION OF A PURE STRAIN OF MAMMALIAN CELLS (EARLE) FOR THE CULTIVATION OF VIRUSES INVITRO .1. 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