{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:17:01Z","timestamp":1789204621257,"version":"build-2803163510"},"reference-count":10,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3394","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1960,1,15]]},"abstract":"<jats:p>Electron microscopy of ultrathin osmium-fixed sections of dental calculus, cut with a diamond knife without prior decalcification, revealed densely mineralized areas entrapping many degenerating microorganisms, within which were deposited similarly electron-dense crystals. Two principal forms of crystals were found, the predominant type being of the same order of magnitude and shape as those found in bone, and showing the typical characteristics of apatite in selected area electron diffraction patterns.<\/jats:p>","DOI":"10.1126\/science.131.3394.156","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T08:04:37Z","timestamp":1160035477000},"page":"156-158","source":"Crossref","is-referenced-by-count":52,"title":["Electronmicroscopy of Dental Calculus"],"prefix":"10.1126","volume":"131","author":[{"given":"F.","family":"Gonzales","sequence":"first","affiliation":[{"name":"Harvard School of Dental Medicine, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. F.","family":"Sognnaes","sequence":"additional","affiliation":[{"name":"Harvard School of Dental Medicine, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"860","volume":"43","year":"1957","unstructured":"GLIMCHER, M.J., MACROMOLECULAR AGGREGATION STATES IN RELATION TO MINERALIZATION - THE COLLAGEN-HYDROXYAPATITE SYSTEM AS STUDIED INVITRO, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 43: 860 (1957).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_2_1","unstructured":"Jackson S. F. Bone Structure and Metabolism (1956)."},{"key":"e_1_2_1_3_1","first-page":"613","volume":"8","year":"1958","unstructured":"LEUNG, S.W., INTERNATIONAL DENTAL JOURNAL 8: 613 (1958).","journal-title":"INTERNATIONAL DENTAL JOURNAL"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/0006-3002(58)90513-4","volume":"28","year":"1958","unstructured":"PAUTARD, FGE, BONE SALTS IN UNICELLULAR ORGANISMS, BIOCHIMICA ET BIOPHYSICA ACTA 28: 514 (1958).","journal-title":"BIOCHIMICA ET BIOPHYSICA ACTA"},{"key":"e_1_2_1_5_1","unstructured":"Pautard F. G. E. Calcification in unicellular organisms Calcification in Biological Systems (1960)."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1111\/j.1749-6632.1955.tb40054.x","volume":"60","year":"1955","unstructured":"ROBINSON, R.A., CRYSTAL-COLLAGEN RELATIONSHIPS IN BONE AS OBSERVED IN THE ELECTRON MICROSCOPE .3. CRYSTAL AND COLLAGEN MORPHOLOGY AS A FUNCTION OF AGE, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 60: 596 (1955).","journal-title":"ANNALS OF THE NEW YORK ACADEMY OF SCIENCES"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.2106\/00004623-195234020-00013","volume":"34","year":"1952","unstructured":"ROBINSON, R. A., JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME 34: 389 (1952).","journal-title":"JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME"},{"key":"e_1_2_1_8_1","unstructured":"Sognnaes R. F. Calcification in Biological Systems (1960)."},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3109\/00016355709041096","volume":"15","year":"1957","unstructured":"TOVBORG JENSEN, A, ACTA ODONTOLOGICA SCANDINAVICA 15: 121 (1957).","journal-title":"ACTA ODONTOLOGICA SCANDINAVICA"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1177\/00220345540330060201","volume":"33","year":"1954","unstructured":"TOVBORG JENSEN, A, J DENT RES 33: 741 (1954).","journal-title":"J DENT RES"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.131.3394.156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T19:53:40Z","timestamp":1704916420000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.131.3394.156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1960,1,15]]},"references-count":10,"journal-issue":{"issue":"3394","published-print":{"date-parts":[[1960,1,15]]}},"alternative-id":["10.1126\/science.131.3394.156"],"URL":"https:\/\/doi.org\/10.1126\/science.131.3394.156","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1960,1,15]]}}}