{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:28:56Z","timestamp":1791206936840,"version":"4.1.0"},"reference-count":16,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5084","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1992,11,6]]},"abstract":"<jats:p>\n            A 9.5-kilobase plasmid of\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            , the causative agent of plague, is required for high virulence when mice are inoculated with the bacterium by subcutaneous injection. Inactivation of the plasmid gene\n            <jats:italic>pla<\/jats:italic>\n            , which encodes a surface protease, increased the median lethal dose of the bacteria for mice by a millionfold. Moreover, cloned\n            <jats:italic>pla<\/jats:italic>\n            was sufficient to restore segregants lacking the entire\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>-<\/jats:sup>\n            bearing plasmid to full virulence. Both\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            strains injected subcutaneously and\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>-<\/jats:sup>\n            mutants injected intravenously reached high titers in liver and spleen of infected mice, whereas\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>-<\/jats:sup>\n            mutants injected subcutaneously failed to do so even though they establish a sustained local infection at the injection site. More inflammatory cells accumulated in lesions caused by the\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>-<\/jats:sup>\n            mutants than in lesions produced by the\n            <jats:italic>pla<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            parent. The Pla protease was shown to be a plasminogen activator with unusual kinetic properties. It can also cleave complement C3 at a specific site.\n          <\/jats:p>","DOI":"10.1126\/science.1439793","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T23:03:06Z","timestamp":1160089386000},"page":"1004-1007","source":"Crossref","is-referenced-by-count":408,"title":["A Surface Protease and the Invasive Character of Plague"],"prefix":"10.1126","volume":"258","author":[{"given":"Ola A.","family":"Sodeinde","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. V. B. K.","family":"Subrahmanyam","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Stark","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Quan","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, CO 80522."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youdi","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon D.","family":"Goguen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1128\/jb.94.1.19-26.1967","volume":"94","year":"1967","unstructured":"BEESLEY, E. 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