{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T05:03:22Z","timestamp":1704949402076},"reference-count":9,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3630","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1964,7,24]]},"abstract":"<jats:p>\n            Cytoplasmic fragments were produced by micromanipulation of cells from a human amnion cell line cultured on coverslips. The cultures were infected with type 1 (Mahoney) poliovirus, and incubated for 7 hours with tritiated uridine (H\n            <jats:sup>3<\/jats:sup>\n            U). Fluorescent antibody to the poliovirus indicated antigenic sites in a number of non-nucleate fragments. By autoradiography the incorporation of H\n            <jats:sup>3<\/jats:sup>\n            U was demonstrated at some of the same sites. The occurrence of poliovirus antigen at the same site as induced synthesis of RNA in non-nucleate cytoplasm of mammalian cells indicates that poliovirus infection and growth occurred independently of immediate contribution from the nucleus.\n          <\/jats:p>","DOI":"10.1126\/science.145.3630.401","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T09:47:29Z","timestamp":1160041649000},"page":"401-403","source":"Crossref","is-referenced-by-count":12,"title":["Poliovirus: Growth in Non-Nucleate Cytoplasm"],"prefix":"10.1126","volume":"145","author":[{"given":"T. Timothy","family":"Crocker","sequence":"first","affiliation":[{"name":"Cancer Research Institute and Department of Medicine, University of California Medical Center, San Francisco 22"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Pfendt","sequence":"additional","affiliation":[{"name":"Cancer Research Institute and Department of Medicine, University of California Medical Center, San Francisco 22"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rex","family":"Spendlove","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Disease Laboratory, California State Department of Public Health, Berkeley 4"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"315","volume":"12","year":"1958","unstructured":"CROCKER, T.T., MIKROSKOPIE 12: 315 (1958).","journal-title":"MIKROSKOPIE"},{"key":"e_1_2_1_2_1","unstructured":"CROCKER T.T. 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THE MICROSCOPIC BEHAVIOR OF ENUCLEATE HUMAN CELL FRAGMENTS, EXPERIMENTAL CELL RESEARCH 19: 332 (1960).","journal-title":"EXPERIMENTAL CELL RESEARCH"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0020-708X(56)90003-5","volume":"1","year":"1956","unstructured":"PELC, S.R., INT J APPL RADIAT IS 1: 172 (1956).","journal-title":"INT J APPL RADIAT IS"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.4049\/jimmunol.90.4.548","volume":"90","year":"1963","unstructured":"SPENDLOVE, R.S., DEVELOPMENT OF VIRAL ANTIGEN AND INFECTIOUS VIRUS IN HELA CELLS INFECTED WITH REOVIRUS, JOURNAL OF IMMUNOLOGY 90: 548 (1963).","journal-title":"JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_9_1","first-page":"1047","volume":"17","year":"1957","unstructured":"ZITCER, E.M., TRANSFORMATION OF CELLS FROM THE NORMAL HUMAN AMNION INTO ESTABLISHED STRAINS, CANCER RESEARCH 17: 1047 (1957).","journal-title":"CANCER RESEARCH"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.145.3630.401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T02:11:31Z","timestamp":1704939091000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.145.3630.401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1964,7,24]]},"references-count":9,"journal-issue":{"issue":"3630","published-print":{"date-parts":[[1964,7,24]]}},"alternative-id":["10.1126\/science.145.3630.401"],"URL":"https:\/\/doi.org\/10.1126\/science.145.3630.401","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1964,7,24]]}}}