{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:52:08Z","timestamp":1776135128946,"version":"3.50.1"},"reference-count":9,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3697","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1965,11,5]]},"abstract":"\n trans<\/jats:italic>\n -Aconitate ion, an inhibitor of the tricarboxylic acid cycle, was identified in range grasses as\n trans<\/jats:italic>\n -aconitic acid, which was isolated in crystalline form. It occurs in surprisingly high concentrations in early-season forage grasses. Dry-weight concentrations of\n trans<\/jats:italic>\n -aconitate vary with season and species; concentrations of between 1 and 2.5 percent are common in mixed pasture grasses, but are higher in certain species such as\n Hordeum leporinum<\/jats:italic>\n (3.5 percent) and\n Phalaris tuberosa<\/jats:italic>\n var.\n stenoptera<\/jats:italic>\n (4.2 percent). Leaves of western larkspur (\n Delphinium hesperium<\/jats:italic>\n ) contain 12.2 percent\n trans<\/jats:italic>\n -aconitate.\n trans<\/jats:italic>\n -Aconitate may be partially responsible for nutritional disorders, such as grass tetany (hypomagnesemia), that occur in grazing cattle in early spring.\n <\/jats:p>","DOI":"10.1126\/science.150.3697.766","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T10:04:55Z","timestamp":1160042695000},"page":"766-767","source":"Crossref","is-referenced-by-count":40,"title":["Trans-Aconitic Acid in Range Grasses in Early Spring"],"prefix":"10.1126","volume":"150","author":[{"given":"R.","family":"Burau","sequence":"first","affiliation":[{"name":"Department of Soils and Plant Nutrition and Kearney Foundation of Soil Science, University of California, Davis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. R.","family":"Stout","sequence":"additional","affiliation":[{"name":"Department of Soils and Plant Nutrition and Kearney Foundation of Soil Science, University of California, Davis"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1128\/jb.85.3.604-613.1963","volume":"85","year":"1963","unstructured":"ALTEKAR, W.W., MICROBIOLOGICAL DISSIMILATION OF TRICARBALLYLATE AND TRANS-ACONITATE, JOURNAL OF BACTERIOLOGY 85: 604 (1963).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1021\/jo01161a013","volume":"13","year":"1948","unstructured":"AMBLER, J.A., THE EFFECT OF PH ON THE STABILITY OF CIS-ACONITIC ACID IN DILUTE SOLUTION, JOURNAL OF ORGANIC CHEMISTRY 13: 399 (1948).","journal-title":"JOURNAL OF ORGANIC CHEMISTRY"},{"key":"e_1_2_1_3_1","first-page":"1193","volume":"192","year":"1961","unstructured":"BURT, A, DIETARY CITRATE AND HYPOMAGNESAEMIA IN RUMINANT, NATURE 192: 1193 (1961).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1021\/ja01238a050","volume":"66","year":"1944","unstructured":"KOLTHOFF, I.M., A new method for the polarographic determination of nitrate, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 66: 1782 (1944).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_5_1","first-page":"98","volume":"1","year":"1948","unstructured":"LUGG, J. W. H., AUSTRALIAN JOURNAL OF SCIENTIFIC RESEARCH SERIES A-PHYSICAL SCIENCES 1: 98 (1948).","journal-title":"AUSTRALIAN JOURNAL OF SCIENTIFIC RESEARCH SERIES A-PHYSICAL SCIENCES"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0031-9422(00)84002-9","volume":"3","year":"1964","unstructured":"MACLENNAN, D. H., PHYTOCHEMISTRY 3: 109 (1964).","journal-title":"PHYTOCHEMISTRY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1128\/jb.78.1.146-146.1959","volume":"78","year":"1959","unstructured":"PISANO, M.A., SELECTIVE ANTIBACTERIAL ACTIVITY OF ACONITIC ACID, JOURNAL OF BACTERIOLOGY 78: 146 (1959).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1021\/ac60089a007","volume":"26","year":"1954","unstructured":"ROBERTS, E.J., IDENTIFICATION AND DETERMINATION OF NONNITROGENOUS ORGANIC ACIDS OF SUGAR CANE BY PARTITION CHROMATOGRAPHY, ANALYTICAL CHEMISTRY 26: 815 (1954).","journal-title":"ANALYTICAL CHEMISTRY"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/S0021-9258(19)51244-3","volume":"180","year":"1949","unstructured":"SAFFRAN, M, INHIBITION OF ACONITASE BY TRANS-ACONITATE, JOURNAL OF BIOLOGICAL CHEMISTRY 180: 1301 (1949).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.150.3697.766","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T08:32:23Z","timestamp":1704961943000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.150.3697.766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1965,11,5]]},"references-count":9,"journal-issue":{"issue":"3697","published-print":{"date-parts":[[1965,11,5]]}},"alternative-id":["10.1126\/science.150.3697.766"],"URL":"https:\/\/doi.org\/10.1126\/science.150.3697.766","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1965,11,5]]}}}