{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:28:13Z","timestamp":1758709693674},"reference-count":22,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3723","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1966,5,6]]},"abstract":"<jats:p>\n            Single tobacco callus cells with or without tobacco mosaic virus inclusion bodies from systemically infected\n            <jats:italic>Nicotiana tabacum plants<\/jats:italic>\n            were grown in microcultures. The culture medium consisted of mineral salts and sucrose; it also contained coconut milk. Out of 100 inclusion-bearing cells and 150 inclusion-free cells, 10 and 70 cultured cells divided; eventually 5 and 65 cells, respectively, formed single cell clones. The 5 clones derived from inclusion-bearing cells, and all but 3 of 40 clones from inclusion-free cells, showed virus inclusions in somne cells. The virus could not be detected in three inclusion-free clones by local lesion assay. The results suggest single-cell culture methods for differentiating virus-free plants from cells of pathogen-infected plants.\n          <\/jats:p>","DOI":"10.1126\/science.152.3723.789","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T10:47:55Z","timestamp":1160045275000},"page":"789-791","source":"Crossref","is-referenced-by-count":6,"title":["Growth in Microculture of Single Tobacco Cells Infected with Tobacco Mosaic Virus"],"prefix":"10.1126","volume":"152","author":[{"given":"Naresh","family":"Chandra","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, University of Wisconsin, Madison 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. C.","family":"Hildebrandt","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, University of Wisconsin, Madison 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"941","volume":"43","year":"1959","unstructured":"BERGMANN, L, J GEN PHYSIOL 43: 941 (1959).","journal-title":"J GEN PHYSIOL"},{"key":"e_1_2_1_2_1","first-page":"614","volume":"52","year":"1965","unstructured":"CHANDRA, N, DISTRIBUTION AND INFECTIVITY OF TOBACCO MOSAIC VIRUS IN TOBACCO TISSUE CULTURES, AMERICAN JOURNAL OF BOTANY 52: 614 (1965).","journal-title":"AMERICAN JOURNAL OF BOTANY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/206325a0","volume":"206","year":"1965","unstructured":"CHANDRA, N, TOBACCO MOSAIC VIRUS INCLUSION BODIES IN TOBACCO TISSUE CULTURES, NATURE 206: 325 (1965).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.141.3585.1059"},{"key":"e_1_2_1_5_1","first-page":"633","volume":"52","year":"1965","unstructured":"HANSEN, A.J., TOBACCO MOSAIC VIRUS IN SINGLE CULTURED CELLS AND IN DIFFERENTIATING TISSUE, AMERICAN JOURNAL OF BOTANY 52: 633 (1965).","journal-title":"AMERICAN JOURNAL OF BOTANY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0042-6822(66)90302-3","volume":"28","year":"1966","unstructured":"HANSEN, A.J., DISTRIBUTION OF TABACCO MOSAIC VIRUS IN PLANT CALLUS CULTURES, VIROLOGY 28: 15 (1966).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_7_1","first-page":"986","volume":"17","year":"1958","unstructured":"HILDEBRANDT, A.C., FEDERATION PROCEEDINGS 17: 986 (1958).","journal-title":"FEDERATION PROCEEDINGS"},{"key":"e_1_2_1_8_1","first-page":"383","volume":"3","year":"1963","unstructured":"Hildebrandt, A. 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