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Since this hapten undergoes a striking increase in fluorescence on binding to light chains (and also on binding to specific antibody), the presence of small amounts of bound hapten could be determined, even in the presence of the high concentrations of unbound hapten required because of the low binding constant.<\/jats:p>","DOI":"10.1126\/science.157.3789.707","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T11:08:12Z","timestamp":1160046492000},"page":"707-709","source":"Crossref","is-referenced-by-count":33,"title":["Specific Binding Activity of Isolated Light Chains of Antibodies"],"prefix":"10.1126","volume":"157","author":[{"given":"T. J.","family":"Yoo","sequence":"first","affiliation":[{"name":"Department of Biochemistry Research, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. 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