{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:19:26Z","timestamp":1758280766514},"reference-count":7,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"3874","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1969,3,28]]},"abstract":"<jats:p>\n            Control rats and rats treated with subcutaneous trypan blue were injected intravenously with denatured albumin-I\n            <jats:sup>125<\/jats:sup>\n            . Lysosome-rich fractions of their livers, when incubated at 22\u00b0C in osmotically protected medium (\n            <jats:italic>p<\/jats:italic>\n            H 7.4), retained their capacity to digest albumin-I\n            <jats:sup>125<\/jats:sup>\n            . The rate of digestion was lower in suspensions prepared from rats treated with trypan blue than in control suspensions, but rates of lysosome breakage were not different. These results and other experimental evidence suggest that trypan blue concentrated within lysosomes can inhibit intralysosomal digestion, probably by inhibition of lysosomal proteinases.\n          <\/jats:p>","DOI":"10.1126\/science.163.3874.1454","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T12:14:21Z","timestamp":1160050461000},"page":"1454-1456","source":"Crossref","is-referenced-by-count":10,"title":["Protein Digestion in Isolated Lysosomes Inhibited by Intralysosomal Trypan Blue"],"prefix":"10.1126","volume":"163","author":[{"given":"M.","family":"Davies","sequence":"first","affiliation":[{"name":"Welsh National School of Medicine, Tenovus Institute for Cancer Research, The Heath, Cardiff, Wales"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. B.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Welsh National School of Medicine, Tenovus Institute for Cancer Research, The Heath, Cardiff, Wales"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Beck","sequence":"additional","affiliation":[{"name":"Welsh National School of Medicine, Tenovus Institute for Cancer Research, The Heath, Cardiff, Wales"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"590","volume":"10","year":"1961","unstructured":"BARKA, T, ACID PHOSPHATASE AND RETICULOENDOTHELIAL SYSTEM, LABORATORY INVESTIGATION 10: 590 (1961).","journal-title":"LABORATORY INVESTIGATION"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.157.3793.1180"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.ph.28.030166.002251","volume":"28","year":"1966","unstructured":"DEDUVE, C, FUNCTIONS OF LYSOSOMES, ANNUAL REVIEW OF PHYSIOLOGY 28: 435 (1966).","journal-title":"ANNUAL REVIEW OF PHYSIOLOGY"},{"key":"e_1_2_1_4_1","first-page":"155","year":"1968","unstructured":"de Duve, C., Interaction of Drugs with Subcellular Components in Animal Cells: 155 (1968).","journal-title":"Interaction of Drugs with Subcellular Components in Animal Cells"},{"key":"e_1_2_1_5_1","first-page":"171","year":"1968","unstructured":"Lloyd, J. 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