{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:00:05Z","timestamp":1774677605867,"version":"3.50.1"},"reference-count":9,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4955","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1990,5,4]]},"abstract":"<jats:p>A cell line has been established in continuous culture of human cerebral cortical neurons obtained from a patient with unilateral megalencephaly, a disorder associated with continued proliferation of immature neuronal cells. When differentiated in the presence of nerve growth factor, 1-isobutyl-3-methylxanthine, and dibutyryl adenosine 3\u2032,5\u2032-monophosphate (cAMP), the cells display mature neuronal morphology with numerous long, extensively branched processes with spines and varicosities. The cells stain positively for neurofilament protein and neuron-specific enolase (selective neuronal markers) but are negative for glial markers, such as glial fibrillary acidic protein, S-100, and myelin basic protein. The cells also stain positively for the neurotransmitters \u03b3-aminobutyric acid (GABA), glutamate, somatostatin, cholecystokinin-8, and vasoactive intestinal polypeptide. These cells may facilitate characterization of neurons in the human central nervous system.<\/jats:p>","DOI":"10.1126\/science.1692158","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T22:10:14Z","timestamp":1160086214000},"page":"603-605","source":"Crossref","is-referenced-by-count":97,"title":["Human Cortical Neuronal Cell Line: Establishment From a Patient with Unilateral Megalencephaly"],"prefix":"10.1126","volume":"248","author":[{"given":"Gabriele V.","family":"Ronnett","sequence":"first","affiliation":[{"name":"Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205."},{"name":"Department of Neuroscience, Pharmacology, and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynda D.","family":"Hester","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Pharmacology, and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey S.","family":"Nye","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Pharmacology, and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Connors","sequence":"additional","affiliation":[{"name":"Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solomon H.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Pharmacology, and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205."},{"name":"Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 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