{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:47:06Z","timestamp":1761518826891},"reference-count":32,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4016","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1971,12,24]]},"abstract":"<jats:p>The active site of various carbonic anhydrases is not blocked by DDT. DDE, or dieldrin. Impairment of catalytic efficiency can only be documented in opalescent test solutions in which the insecticides are present in excess of their solubility limit. These slowly forming precipitates occlude enzyme from solution and furnish a physical explanation of the supposed inhibition. The amounts occluded depend, among other things, on the conformational and topographical characteristics of the particular carbonic anhydrase molecules.<\/jats:p>","DOI":"10.1126\/science.174.4016.1336","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T13:17:43Z","timestamp":1160054263000},"page":"1336-1339","source":"Crossref","is-referenced-by-count":20,"title":["Carbonic Anhydrase Interaction with DDT, DDE, and Dieldrin"],"prefix":"10.1126","volume":"174","author":[{"given":"Y.","family":"Pocker","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle 98105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. M.","family":"Beug","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle 98105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. R.","family":"Ainardi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Washington, Seattle 98105"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"349","volume":"29","year":"1970","unstructured":"BITMAN, J, FED P FASEB 29: 349 (1970).","journal-title":"FED P FASEB"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/224044a0","volume":"224","year":"1969","unstructured":"BITMAN, J, DDT INDUCES A DECREASE IN EGGSHELL CALCIUM, NATURE 224: 44 (1969).","journal-title":"NATURE"},{"key":"e_1_2_1_3_1","first-page":"545","volume":"5","year":"1961","unstructured":"Davis, R. P., The Enzymes 5: 545 (1961).","journal-title":"The Enzymes"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1039\/j29660000696","year":"1966","unstructured":"ENGLAND, B. D., JOURNAL OF THE CHEMICAL SOCIETY B-PHYSICAL ORGANIC: 696 (1966).","journal-title":"JOURNAL OF THE CHEMICAL SOCIETY B-PHYSICAL ORGANIC"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"3502","DOI":"10.1016\/S0021-9258(18)48696-6","volume":"238","year":"1963","unstructured":"GIBBONS, B.H., RATE OF HYDRATION OF CARBON DIOXIDE AND DEHYDRATION OF CARBONIC ACID AT 25 DEGREES, JOURNAL OF BIOLOGICAL CHEMISTRY 238: 3502 (1963).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"858","DOI":"10.3382\/ps.0470858","volume":"47","year":"1968","unstructured":"HEALD, P.J., SHELL STRENGTH AND CARBONIC ANHYDRASE ACTIVITY OF SHELL GLAND OF DOMESTIC FOWL, POULTRY SCIENCE 47: 858 (1968).","journal-title":"POULTRY SCIENCE"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1016\/S0021-9258(18)48695-4","volume":"238","year":"1963","unstructured":"HO, C, KINETICS OF HYDRATION OF CARBON DIOXIDE AT 25 DEGREES, JOURNAL OF BIOLOGICAL CHEMISTRY 238: 3499 (1963).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_8_1","first-page":"626","year":"1965","unstructured":"Keller, H., Methods of Enzymatic Analysis: 626 (1965).","journal-title":"Methods of Enzymatic Analysis"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF00590402","volume":"39","year":"1952","unstructured":"KELLER, H, DIE BESTIMMUNG KLEINSTER MENGEN DDT AUF ENZYMANALYTISCHEM WEGE, NATURWISSENSCHAFTEN 39: 109 (1952).","journal-title":"NATURWISSENSCHAFTEN"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/j.1432-1033.1967.tb19552.x","volume":"3","year":"1968","unstructured":"LINDSKOG, S, ON INTERACTION OF BOVINE COBALT CARBONIC ANHYDRASE WITH SULFONAMIDES, EUROPEAN JOURNAL OF BIOCHEMISTRY 3: 453 (1968).","journal-title":"EUROPEAN JOURNAL OF BIOCHEMISTRY"},{"key":"e_1_2_1_11_1","first-page":"121","year":"1964","unstructured":"Malmstrom, B. G., Structure and Activity of Enzymes: 121 (1964).","journal-title":"Structure and Activity of Enzymes"},{"key":"e_1_2_1_12_1","first-page":"389","volume":"130","year":"1960","unstructured":"MAREN, T.H., A KINETIC ANALYSIS OF CARBONIC ANHYDRASE INHIBITION, JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 130: 389 (1960).","journal-title":"JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1152\/physrev.1967.47.4.595","volume":"47","year":"1967","unstructured":"MAREN, T.H., CARBONIC ANHYDRASE - CHEMISTRY PHYSIOLOGY AND INHIBITION, PHYSIOLOGICAL REVIEWS 47: 595 (1967).","journal-title":"PHYSIOLOGICAL REVIEWS"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1113\/jphysiol.1933.sp003077","volume":"80","year":"1933","unstructured":"MELDRUM, N.U., JOURNAL OF PHYSIOLOGY-LONDON 80: 113 (1933).","journal-title":"JOURNAL OF PHYSIOLOGY-LONDON"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0470-72"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"2535","DOI":"10.1021\/bi00887a037","volume":"4","year":"1965","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .I. KINETIC STUDIES OF ENZYME-CATALYZED HYDRATION OF ACETALDEHYDE, BIOCHEMISTRY 4: 2535 (1965).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1021\/bi00853a037","volume":"6","year":"1967","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .2. KINETIC STUDIES OF ENZYME-CATALYZED HYDRATION OF PYRIDINE ALDEHYDES, BIOCHEMISTRY 6: 239 (1967).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1021\/bi00855a005","volume":"6","year":"1967","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .3. KINETIC STUDIES OF ENZYME-CATALYZED HYDROLYSIS OF P-NITROPHENYL ACETATE, BIOCHEMISTRY 6: 668 (1967).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1021\/bi00843a042","volume":"7","year":"1968","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .4. KINETIC STUDIES OF ENZYME-CATALYZED HYDROLYSES OF P-NITROPHENYL ESTERS, BIOCHEMISTRY 7: 1202 (1968).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1021\/bi00845a050","volume":"7","year":"1968","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .V. KINETIC STUDIES OF ENZYME-CATALYZED HYDRATIONS OF ALIPHATIC ALDEHYDES, BIOCHEMISTRY 7: 1995 (1968).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1021\/bi00848a034","volume":"7","year":"1968","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .6. KINETIC STUDIES OF NONCOMPETITIVE INHIBITION OF ENZYME-CATALYZED HYDROLYSIS OF P-NITROPHENYL ACETATE, BIOCHEMISTRY 7: 2936 (1968).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1021\/bi00849a001","volume":"7","year":"1968","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE .7. KINETIC STUDIES OF ESTERASE ACTIVITY AND COMPETITIVE INHIBITION BY SUBSTRATE ANALOGS, BIOCHEMISTRY 7: 3021 (1968).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_23_1","first-page":"1809","volume":"87","year":"1965","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF CARBONIC ANHYDRASE FROM ERYTHROCYTES . ENZYME-CATALYZED HYDRATION OF ACETALDEHYDE, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 87: 1809 (1965).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"5497","DOI":"10.1021\/ja00951a049","volume":"87","year":"1965","unstructured":"POCKER, Y, CATALYTIC VERSATILITY OF ERYTHROCYTE CARBONIC ANHYDRASE . ENZYME-CATALYZED HYDROLYSIS OF P-NITROPHENYL ACETATE, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 87: 5497 (1965).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_25_1","first-page":"327","volume":"4","year":"1964","unstructured":"Pocker, Y., Abstracts. International Congress on Biochemistry 4: 327 (1964).","journal-title":"Abstracts. International Congress on Biochemistry"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0021-9258(18)91392-X","volume":"239","year":"1964","unstructured":"RICKLI, E.E., CARBONIC ANHYDRASES FROM HUMAN ERYTHROCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 239: 1065 (1964).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1038\/2201098a0","volume":"220","year":"1968","unstructured":"RISEBROUGH, R.W., POLYCHLORINATED BIPHENYLS IN GLOBAL ECOSYSTEM, NATURE 220: 1098 (1968).","journal-title":"NATURE"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1042\/bj0400319","volume":"40","year":"1946","unstructured":"ROUGHTON, FJW, THE EFFECT OF SUBSTRATE CONCENTRATION, PH AND OTHER FACTORS UPON THE ACTIVITY OF CARBONIC ANHYDRASE, BIOCHEMICAL JOURNAL 40: 319 (1946).","journal-title":"BIOCHEMICAL JOURNAL"},{"key":"e_1_2_1_29_1","first-page":"96","year":"1944","unstructured":"ROUGHTON, FJW, HARVEY LECTURES: 96 (1944).","journal-title":"HARVEY LECTURES"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/0006-291X(64)90445-0","volume":"14","year":"1964","unstructured":"TASHIAN, R.E., ESTERASE + HYDRASE ACTIVITY OF CARBONIC ANHYDRASE-I FROM PRIMATE ERYTHROCYTES, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 14: 256 (1964).","journal-title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"},{"key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1016\/S0021-9258(18)95800-X","volume":"242","year":"1967","unstructured":"VERPOORTE, J.A., ESTERASE ACTIVITIES OF HUMAN CARBONIC ANHYDRASES B AND C, JOURNAL OF BIOLOGICAL CHEMISTRY 242: 4221 (1967).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0021-9258(18)51011-5","volume":"176","year":"1948","unstructured":"WILBUR, K.M., ELECTROMETRIC AND COLORIMETRIC DETERMINATION OF CARBONIC ANHYDRASE, JOURNAL OF BIOLOGICAL CHEMISTRY 176: 147 (1948).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.174.4016.1336","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T15:04:38Z","timestamp":1704985478000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.174.4016.1336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,12,24]]},"references-count":32,"journal-issue":{"issue":"4016","published-print":{"date-parts":[[1971,12,24]]}},"alternative-id":["10.1126\/science.174.4016.1336"],"URL":"https:\/\/doi.org\/10.1126\/science.174.4016.1336","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,12,24]]}}}