{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:34:15Z","timestamp":1792251255299,"version":"4.4.0"},"reference-count":19,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4117","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1973,12,14]]},"abstract":"<jats:p>\n            Factor VIII corrects both the clotting defect in hemophilia A and an abnormality of platelet aggregation in von Willebrand's disease. These two activities of factor VIII (antihemophilic factor and von Willebrand factor) are both detected in the void volume when human plasma or cryoprecipitate is chromatographed on Bio-Gel 5M under conditions of isotonic salt concentration. In contrast, antihemophilic factor procoagulant activity is detected with proteins of lower molecular weight when the chromatography is performed with a buffer containing 0.8\n            <jats:italic>M<\/jats:italic>\n            NaCl. In this way, the two activities of factor VIII can be dissociated. It remains to be determined whether these components are separate molecules associated as a complex of high molecular weight in plasma or whether they are subunits of a complex macromolecule.\n          <\/jats:p>","DOI":"10.1126\/science.182.4117.1149","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T14:05:45Z","timestamp":1160057145000},"page":"1149-1151","source":"Crossref","is-referenced-by-count":103,"title":["Von Willebrand Factor: Dissociation from Antihemophilic Factor Procoagulant Activity"],"prefix":"10.1126","volume":"182","author":[{"given":"Harvey J.","family":"Weiss","sequence":"first","affiliation":[{"name":"Department of Medicine, Roosevelt Hospital, New York 10019, and Columbia University College of Physicians and Surgeons, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon W.","family":"Hoyer","sequence":"additional","affiliation":[{"name":"Departmlent of Medicine, University of Connecticut School of Medicine, Farmington 06032"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/newbio236104a0","volume":"236","year":"1972","unstructured":"BOUMA, B.N., IMMUNOLOGICAL CHARACTERIZATION OF PURIFIED ANTI-HEMOPHILIC FACTOR A (FACTOR-VIII) WHICH CORRECTS ABNORMAL PLATELET RETENTION IN VON WILLEBRANDS DISEASE, NATURE-NEW BIOLOGY 236: 104 (1972).","journal-title":"NATURE-NEW BIOLOGY"},{"key":"e_1_2_1_2_1","first-page":"488","volume":"26","year":"1967","unstructured":"HERSHGOLD, E, NATIVE AND PURIFIED FACTOR 8 - MOLECULAR AND ELECTRON MICROSCOPICAL PROPERTIES AND A COMPARISON WITH HEMOPHILIC PLASMA, FEDERATION PROCEEDINGS 26: 488 (1967).","journal-title":"FEDERATION PROCEEDINGS"},{"key":"e_1_2_1_3_1","first-page":"362","volume":"26","year":"1971","unstructured":"HOWARD, M.A., RISTOCETIN - NEW TOOL IN INVESTIGATION OF PLATELET AGGREGATION, THROMBOSIS ET DIATHESIS HAEMORRHAGICA 26: 362 (1971).","journal-title":"THROMBOSIS ET DIATHESIS HAEMORRHAGICA"},{"key":"e_1_2_1_4_1","first-page":"822","volume":"80","year":"1972","unstructured":"HOYER, L.W., IMMUNOLOGICAL STUDIES OF ANTIHEMOPHILIC FACTOR (AHF, FACTOR-VIII) .4. RADIOIMMUNOASSAY OF AHF ANTIGEN, JOURNAL OF LABORATORY AND CLINICAL MEDICINE 80: 822 (1972).","journal-title":"JOURNAL OF LABORATORY AND CLINICAL MEDICINE"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1172\/JCI105991","volume":"48","year":"1969","unstructured":"KASS, L, STUDIES ON PURIFICATION OF ANTIHEMOPHILIC FACTOR (FACTOR 8) .I. PRECIPITATION OF ANTIHEMOPHILIC FACTOR BY CONCANAVALIN A, JOURNAL OF CLINICAL INVESTIGATION 48: 351 (1969).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_6_1","first-page":"502","volume":"27","year":"1972","unstructured":"OWEN, W.G., ANTIHEMOPHILIC FACTOR - SEPARATION OF AN ACTIVE FRAGMENT FOLLOWING DISSOCIATION BY SALTS OR DETERGENTS, THROMBOSIS ET DIATHESIS HAEMORRHAGICA 27: 502 (1972).","journal-title":"THROMBOSIS ET DIATHESIS HAEMORRHAGICA"},{"key":"e_1_2_1_7_1","first-page":"22","volume":"21","year":"1963","unstructured":"RAPAPORT, S.I., BLOOD 21: 22 (1963).","journal-title":"BLOOD"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1172\/JCI106055","volume":"48","year":"1969","unstructured":"RATNOFF, O.D., STUDIES ON PURIFICATION OF ANTIHEMOPHILIC FACTOR (FACTOR-8) .2. SEPARATION OF PARTIALLY PURIFIED ANTIHEMOPHILIC FACTOR BY GEL FILTRATION OF PLASMA, JOURNAL OF CLINICAL INVESTIGATION 48: 957 (1969).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1182\/blood.V42.5.737.737","volume":"42","year":"1973","unstructured":"RICK, M.E., IMMUNOLOGICAL STUDIES OF ANTIHEMOPHILIC FACTOR (AHF, FACTOR-VIII) .5. IMMUNOLOGICAL PROPERTIES OF AHF SUBUNITS PRODUCED BY SALT DISSOCIATION, BLOOD 42: 737 (1973).","journal-title":"BLOOD"},{"key":"e_1_2_1_10_1","first-page":"564","volume":"21","year":"1973","unstructured":"RICK, M.E., CLIN RES 21: 564 (1973).","journal-title":"CLIN RES"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.171.3967.196"},{"key":"e_1_2_1_12_1","first-page":"939","volume":"40","year":"1972","unstructured":"WEISS, H.J., FURTHER STUDIES ON VONWILLEBRANDS VONWILLEBRAND FACTOR (FACTOR-VIII) - RISTOCETIN-INDUCED PLATELET AGGREGATION, BLOOD-THE JOURNAL OF HEMATOLOGY 40: 939 (1972).","journal-title":"BLOOD-THE JOURNAL OF HEMATOLOGY"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1182\/blood.V41.6.809.809","volume":"41","year":"1973","unstructured":"WEISS, H.J., PROPERTIES OF PLATELET RETENTION (VONWILLEBRANDS WILLEBRAND) FACTOR AND ITS SIMILARITY TO ANTIHEMOPHILIC FACTOR (AHF), BLOOD 41: 809 (1973).","journal-title":"BLOOD"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1365-2141.1970.tb01421.x","volume":"18","year":"1970","unstructured":"WEISS, H.J., MOLECULAR FORMS OF ANTIHAEMOPHILIC GLOBULIN IN PLASMA, CRYOPRECIPITATE AND AFTER THROMBIN ACTIVATION, BRITISH JOURNAL OF HAEMATOLOGY 18: 89 (1970).","journal-title":"BRITISH JOURNAL OF HAEMATOLOGY"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1172\/JCI105903","volume":"47","year":"1968","unstructured":"WEISS, H.J., EFFECT OF SALICYLATES ON HEMOSTATIC PROPERTIES OF PLATELETS IN MAN, JOURNAL OF CLINICAL INVESTIGATION 47: 2169 (1968).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1172\/JCI107464","volume":"52","year":"1973","unstructured":"WEISS, H.J., DEFECTIVE RISTOCETIN-INDUCED PLATELET-AGGREGATION IN VON VONWILLEBRANDS DISEASE AND ITS CORRECTION BY FACTOR-VIII, JOURNAL OF CLINICAL INVESTIGATION 52: 2697 (1973).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1172\/JCI107465","volume":"52","year":"1973","unstructured":"WEISS, H.J., QUANTITATIVE ASSAY OF A PLASMA FACTOR DEFICIENT IN VONWILLEBRANDS DISEASE THAT IS NECESSARY FOR PLATELET-AGGREGATION - RELATIONSHIP TO FACTOR-VIII PROCOAGULANT ACTIVITY AND ANTIGEN CONTENT, JOURNAL OF CLINICAL INVESTIGATION 52: 2708 (1973).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_18_1","first-page":"212","volume":"27","year":"1972","unstructured":"WEISS, H.J., SEPARATION OF SUBUNITS OF ANTIHEMOPHILIC FACTOR (AHF) BY AGAROSE-GEL CHROMATOGRAPHY, THROMBOSIS ET DIATHESIS HAEMORRHAGICA 27: 212 (1972).","journal-title":"THROMBOSIS ET DIATHESIS HAEMORRHAGICA"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1172\/JCI106480","volume":"50","year":"1971","unstructured":"ZIMMERMAN, T.S., IMMUNOLOGIC DIFFERENTIATION OF CLASSIC HEMOPHILIA (FACTOR-VIII DEFICIENCY) AND VON-WILLEBRANDS DISEASE - WITH OBSERVATIONS ON COMBINED DEFICIENCIES OF ANTIHEMOPHILIC FACTOR AND PROACCELERIN (FACTOR V) AND ON AN ACQUIRED CIRCULATING ANTICOAGULANT AGAINST ANTIHEMOPHILIC FACTOR, JOURNAL OF CLINICAL INVESTIGATION 50: 244 (1971).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.182.4117.1149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T20:50:34Z","timestamp":1705006234000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.182.4117.1149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,12,14]]},"references-count":19,"journal-issue":{"issue":"4117","published-print":{"date-parts":[[1973,12,14]]}},"alternative-id":["10.1126\/science.182.4117.1149"],"URL":"https:\/\/doi.org\/10.1126\/science.182.4117.1149","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,12,14]]}}}