{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:09:04Z","timestamp":1791043744809,"version":"4.1.0"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4968","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1990,8,3]]},"abstract":"<jats:p>\n            <jats:italic>Yersinia<\/jats:italic>\n            is the genus of bacteria that is the causative agent in plague or the black death, and on several occasions this organism has killed a significant portion of the world's population. An essential virulence determinant of\n            <jats:italic>Yersinia<\/jats:italic>\n            was shown to be a protein tyrosine phosphatase. The recombinant 50-kilodalton\n            <jats:italic>Yersinia<\/jats:italic>\n            phosphatase had a specificity for removal of phosphate from Tyr-containing as opposed to Ser\/Thr-containing phosphopeptides and proteins. Site-directed mutagenesis was used to show that the\n            <jats:italic>Yersinia<\/jats:italic>\n            phosphatase possesses an essential Cys residue required for catalysis. Amino acids surrounding an essential Cys residue are highly conserved, as are other amino acids in the\n            <jats:italic>Yersinia<\/jats:italic>\n            and mammalian protein tyrosine phosphatases, suggesting that they use a common catalytic mechanism.\n          <\/jats:p>","DOI":"10.1126\/science.2166336","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T22:10:14Z","timestamp":1160086214000},"page":"553-556","source":"Crossref","is-referenced-by-count":437,"title":["Protein Tyrosine Phosphatase Activity of an Essential Virulence Determinant in\n            <i>Yersinia<\/i>"],"prefix":"10.1126","volume":"249","author":[{"given":"Kunliang","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Biochemistry, and Walther Cancer Institute, Purdue University, West Lafayette, IN 47907."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack E.","family":"Dixon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, and Walther Cancer Institute, Purdue University, West Lafayette, IN 47907."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3541204"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1365-2958.1988.tb00025.x","volume":"2","year":"1988","unstructured":"BOLIN, I, THE PLASMID-ENCODED YOP2B PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS IS A VIRULENCE DETERMINANT REGULATED BY CALCIUM AND TEMPERATURE AT THE LEVEL OF TRANSCRIPTION, MOLECULAR MICROBIOLOGY 2: 237 (1988).","journal-title":"MOLECULAR MICROBIOLOGY"},{"key":"e_1_2_1_3_1","first-page":"1600","year":"1985","unstructured":"Butler, T., Textbook of Medicine: 1600 (1985).","journal-title":"Textbook of Medicine"},{"key":"e_1_2_1_4_1","first-page":"7182","volume":"85","year":"1988","unstructured":"CHARBONNEAU, H, THE LEUKOCYTE COMMON ANTIGEN (CD45) - A PUTATIVE RECEPTOR-LINKED PROTEIN TYROSINE PHOSPHATASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 7182 (1988).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_5_1","first-page":"5252","volume":"86","year":"1989","unstructured":"CHARBONNEAU, H, HUMAN-PLACENTA PROTEIN-TYROSINE-PHOSPHATASE - AMINO-ACID SEQUENCE AND RELATIONSHIP TO A FAMILY OF RECEPTOR-LIKE PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 5252 (1989).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_6_1","first-page":"5257","volume":"86","year":"1989","unstructured":"COOL, D.E., CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 5257 (1989).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1111\/j.1365-2958.1989.tb00129.x","volume":"3","year":"1989","unstructured":"CORNELIS, G.R., THE YERSINIA-YOP REGULON, MOLECULAR MICROBIOLOGY 3: 1455 (1989).","journal-title":"MOLECULAR MICROBIOLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0092-8674(89)90414-5","volume":"58","year":"1989","unstructured":"DUNPHY, W.G., FISSION YEAST P13 BLOCKS MITOTIC ACTIVATION AND TYROSINE DEPHOSPHORYLATION OF THE XENOPUS CDC2 PROTEIN-KINASE, CELL 58: 181 (1989).","journal-title":"CELL"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/342039a0","volume":"342","year":"1989","unstructured":"GOULD, K.L., TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS, NATURE 342: 39 (1989).","journal-title":"NATURE"},{"key":"e_1_2_1_10_1","first-page":"1501","volume":"87","year":"1990","unstructured":"GUAN, K.L., CLONING AND EXPRESSION OF A PROTEIN-TYROSINE-PHOSPHATASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 1501 (1990).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_11_1","unstructured":"GUAN K.L. unpublished data."},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/0092-8674(89)90496-0","volume":"58","year":"1989","unstructured":"HUNTER, T, PROTEIN-TYROSINE PHOSPHATASES - THE OTHER SIDE OF THE COIN, CELL 58: 1013 (1989).","journal-title":"CELL"},{"key":"e_1_2_1_13_1","unstructured":"Maniatis T. 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