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Deletion of the 50-amino acid tract (residues 317 to 366) had no effect on TSH binding or on TSH and thyroid-stimulating immunoglobulin (TSI) biological activities. In contrast, either deletion or substitution of the eight-amino acid region (residues 38 to 45) abolished these activities. This eight-amino acid tract near the amino terminus of the TSH receptor appears to be an important site of interaction for both TSH and TSI.<\/jats:p>","DOI":"10.1126\/science.2169649","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T22:10:14Z","timestamp":1160086214000},"page":"1423-1425","source":"Crossref","is-referenced-by-count":110,"title":["An Insertion in the Human Thyrotropin Receptor Critical for High Affinity Hormone Binding"],"prefix":"10.1126","volume":"249","author":[{"given":"Harry L.","family":"Wadsworth","sequence":"first","affiliation":[{"name":"Department of Medicine and the Thyroid Molecular Biology Unit, Veterans Administration Medical Center, San Francisco, CA 94121, and Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregorio D.","family":"Chazenbalk","sequence":"additional","affiliation":[{"name":"Department of Medicine and the Thyroid Molecular Biology Unit, Veterans Administration Medical Center, San Francisco, CA 94121, and Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Nagayama","sequence":"additional","affiliation":[{"name":"Department of Medicine and the Thyroid Molecular Biology Unit, Veterans Administration Medical Center, San Francisco, CA 94121, and Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Medicine and the Thyroid Molecular Biology Unit, Veterans Administration Medical Center, San Francisco, CA 94121, and Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil","family":"Rapoport","sequence":"additional","affiliation":[{"name":"Department of Medicine and the Thyroid Molecular Biology Unit, Veterans Administration Medical Center, San Francisco, CA 94121, and Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2315699"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1210\/endo-127-3-1240","volume":"127","year":"1990","unstructured":"Chazenbalk, G. 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