{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:35:58Z","timestamp":1775151358550,"version":"3.50.1"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4795","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1987,3,20]]},"abstract":"<jats:p>\n            Reverse transcriptase activity of the human immunodeficiency virus (HIV) was blocked in vitro by immunoglobulin G (IgG) derived from certain individuals infected with this retrovirus. A heterogeneous immune response for inhibition of enzyme function was noted. Catalytic activity was depressed by 50% or more with the use of 10 micrograms of IgG from 11 of 16 HIV-seropositive asymptomatic carriers, but from 0 of 8 seronegative controls and 2 of 12 patients with acquired immune deficiency syndrome (AIDS) or the AIDS-related complex (ARC). The inhibitor was confined to the F(ab\u2032)\n            <jats:sub>2<\/jats:sub>\n            fragment. It was not directed against the poly(rA) \u22c5 oligo(dT) template, nor against major envelope or structural viral antigens, and did not cross-react with bacterial, avian, or other mammalian DNA polymerases. It did not correlate with recognition of polymerase antigens by radioimmunoprecipitation. Loss of this inhibitor may be associated with development of clinical disease. Ten asymptomatic HIV-seropositive carriers with high titers of IgG antibodies to reverse transcriptase were followed for a mean of 3 years. All of four lost inhibitory capability prior to development of AIDS or ARC, while titers persist in the six who remain clinically healthy.\n          <\/jats:p>","DOI":"10.1126\/science.2435004","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:58Z","timestamp":1160080138000},"page":"1501-1504","source":"Crossref","is-referenced-by-count":45,"title":["Characterization and Clinical Association of Antibody Inhibitory to HIV Reverse Transcriptase Activity"],"prefix":"10.1126","volume":"235","author":[{"given":"Jeffrey","family":"Laurence","sequence":"first","affiliation":[{"name":"Laboratory for AIDS Research, Department of Medicine, The New York Hospital-Cornell Medical Center, Division of Hematology-Oncology, New York, NY 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Saunders","sequence":"additional","affiliation":[{"name":"Laboratory for AIDS Research, Department of Medicine, The New York Hospital-Cornell Medical Center, Division of Hematology-Oncology, New York, NY 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Kulkosky","sequence":"additional","affiliation":[{"name":"Laboratory for AIDS Research, Department of Medicine, The New York Hospital-Cornell Medical Center, Division of Hematology-Oncology, New York, NY 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"920","volume":"68","year":"1971","unstructured":"AARONSON, S.A., ANTIBODY TO RNA DEPENDENT DNA POLYMERASE OF MAMMALIAN C-TYPE RNA TUMOR VIRUSES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 920 (1971).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1182\/blood.V69.1.331.331","volume":"69","year":"1987","unstructured":"ALLAN, J.S., IMMUNOGENIC NATURE OF A POL GENE-PRODUCT OF HTLV-III LAV, BLOOD 69: 331 (1987).","journal-title":"BLOOD"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2986290"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/jvi.34.1.168-177.1980","volume":"34","year":"1980","unstructured":"BAUER, G, SPECIFIC ANTIGENIC RELATIONSHIPS BETWEEN THE RNA-DEPENDENT DNA-POLYMERASES OF AVIAN RETICULOENDOTHELIOSIS VIRUSES AND MAMMALIAN TYPE-C RETROVIRUSES, JOURNAL OF VIROLOGY 34: 168 (1980).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1016\/S0021-9258(18)69752-2","volume":"234","year":"1959","unstructured":"FAHEY, J.L., HUMAN GAMMA-GLOBULIN FRACTIONATION ON ANION EXCHANGE CELLULOSE COLUMNS, JOURNAL OF BIOLOGICAL CHEMISTRY 234: 2645 (1959).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_6_1","first-page":"278","year":"1977","unstructured":"GOOD, A.H., METHODS IN IMMUNOLOGY: 278 (1977).","journal-title":"METHODS IN IMMUNOLOGY"},{"key":"e_1_2_1_7_1","first-page":"2203","volume":"68","year":"1971","unstructured":"GOODMAN, N.C., DISTINGUISHING REVERSE TRANSCRIPTASE OF AN RNA TUMOR VIRUS FROM OTHER KNOWN DNA POLYMERASES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 2203 (1971).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1093\/infdis\/153.4.736","volume":"153","year":"1986","unstructured":"GROOPMAN, J. E., JOURNAL OF INFECTIOUS DISEASES 153: 736 (1986).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3012778"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1128\/jvi.13.2.448-454.1974","volume":"13","year":"1974","unstructured":"HOLLIS, V.W., DETECTION OF NATURALLY OCCURRING ANTIBODIES TO RNA-DEPENDENT DNA-POLYMERASE OF MURINE LEUKEMIA-VIRUS IN KIDNEY ELUATES OF AKR MICE, JOURNAL OF VIROLOGY 13: 448 (1974).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/0042-6822(78)90393-8","volume":"91","year":"1978","unstructured":"JACQUEMIN, P.C., ANTIBODY-RESPONSE IN CATS TO FELINE LEUKEMIA-VIRUS REVERSE-TRANSCRIPTASE UNDER NATURAL CONDITIONS OF EXPOSURE TO THE VIRUS, VIROLOGY 91: 472 (1978).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/299592a0","volume":"299","year":"1982","unstructured":"LERNER, R.A., TAPPING THE IMMUNOLOGICAL REPERTOIRE TO PRODUCE ANTIBODIES OF PREDETERMINED SPECIFICITY, NATURE 299: 592 (1982).","journal-title":"NATURE"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200935"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0042-6822(86)90260-6","volume":"150","year":"1986","unstructured":"RASHEED, S, VIRUS-NEUTRALIZING ACTIVITY, SEROLOGIC HETEROGENEITY, AND RETROVIRUS ISOLATION FROM HOMOSEXUAL MEN IN THE LOS-ANGELES AREA, VIROLOGY 150: 1 (1986).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1056\/NEJM198601093140232","volume":"314","year":"1986","unstructured":"REDFIELD, R.R., THE REED,WALTER STAGING CLASSIFICATION FOR HTLV-III\/LAV INFECTION, NEW ENGLAND JOURNAL OF MEDICINE 314: 131 (1986).","journal-title":"NEW ENGLAND JOURNAL OF MEDICINE"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/0006-291X(84)90696-X","volume":"121","year":"1984","unstructured":"REY, M.A., CHARACTERIZATION OF THE RNA DEPENDENT DNA-POLYMERASE OF A NEW HUMAN T-LYMPHOTROPIC RETROVIRUS (LYMPHADENOPATHY ASSOCIATED VIRUS), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 121: 126 (1984).","journal-title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1021\/bi00632a010","volume":"16","year":"1977","unstructured":"ROBERTGUROFF, M, DNA POLYMERASE-GAMMA OF HUMAN LYMPHOBLASTS, BIOCHEMISTRY 16: 2866 (1977).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/316072a0","volume":"316","year":"1985","unstructured":"ROBERTGUROFF, M, HTLV-III-NEUTRALIZING ANTIBODIES IN PATIENTS WITH AIDS AND AIDS-RELATED COMPLEX, NATURE 316: 72 (1985).","journal-title":"NATURE"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3010464"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1128\/jvi.58.1.9-16.1986","volume":"58","year":"1986","unstructured":"STEIMER, K.S., RECOMBINANT POLYPEPTIDE FROM THE ENDONUCLEASE REGION OF THE ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME RETROVIRUS POLYMERASE (POL) GENE DETECTS SERUM ANTIBODIES IN MOST INFECTED INDIVIDUALS, JOURNAL OF VIROLOGY 58: 9 (1986).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2418504"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1128\/jvi.26.3.750-761.1978","volume":"26","year":"1978","unstructured":"WITTE, O.N., RELATIONSHIP OF RETROVIRUS POLYPROTEIN CLEAVAGES TO VIRION MATURATION STUDIED WITH TEMPERATURE-SENSITIVE MURINE-LEUKEMIA-VIRUS MUTANTS, JOURNAL OF VIROLOGY 26: 750 (1978).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1128\/jvi.28.1.34-44.1978","volume":"28","year":"1978","unstructured":"WONG, T.C., ISOLATION AND CHARACTERIZATION OF A VIRUS-SPECIFIC RIBONUCLEOPROTEIN COMPLEX FROM RETICULOENDOTHELIOSIS VIRUS-TRANSFORMED CHICKEN BONE-MARROW CELLS, JOURNAL OF VIROLOGY 28: 34 (1978).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_24_1","first-page":"1438","volume":"37","year":"1977","unstructured":"WUU, K.D., INHIBITION OF REVERSE-TRANSCRIPTASE OF BOVINE LEUKEMIA-VIRUS BY ANTIBODY IN SERA FROM LEUKEMIC CATTLE AND IMMUNOLOGICAL CHARACTERIZATION OF ENZYME, CANCER RESEARCH 37: 1438 (1977).","journal-title":"CANCER RESEARCH"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.2435004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T15:48:27Z","timestamp":1705074507000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.2435004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,3,20]]},"references-count":24,"journal-issue":{"issue":"4795","published-print":{"date-parts":[[1987,3,20]]}},"alternative-id":["10.1126\/science.2435004"],"URL":"https:\/\/doi.org\/10.1126\/science.2435004","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,3,20]]}}}