{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:17:47Z","timestamp":1779340667658,"version":"3.51.4"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4830","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1987,11,20]]},"abstract":"<jats:p>\n            Autoantibodies to aminoacyl-transfer RNA (tRNA) synthetases are common in the human autoimmune diseases polymyositis and dermatomyositis. Sera of the PL-12 specificity contain separate antibodies reacting with alanyl-tRNA synthetase and alanine tRNA (tRNA\n            <jats:sup>Ala<\/jats:sup>\n            ). The antibodies to tRNA recognize at least six distinguishable human tRNA\n            <jats:sup>Ala<\/jats:sup>\n            species grouped into two sequence families. The antibody-reactive determinants on the tRNA were identified through ribonuclease protection and oligonucleotide binding experiments. The antibody binding site is a seven- to nine-nucleotide sequence containing the anticodon loop and requires an intact anticodon. No requirement for anticodon stem structure or sequence is observed, although the 5\u2032 portion of the stem is protected from nuclease attack. Antibodies from several patients appear to share the same specificitym, indicating that the antibodies are induced by a unique sequence feature in the immunogen.\n          <\/jats:p>","DOI":"10.1126\/science.2446387","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:58Z","timestamp":1160080138000},"page":"1116-1119","source":"Crossref","is-referenced-by-count":47,"title":["Autoreactive Epitope Defined as the Anticodon Region of Alanine Transfer RNA"],"prefix":"10.1126","volume":"238","author":[{"given":"Christopher C.","family":"Bunn","sequence":"first","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael B.","family":"Mathews","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"751","volume":"2","year":"1971","unstructured":"BARRELL, B.G., PROCEDURES NUCLEIC A 2: 751 (1971).","journal-title":"PROCEDURES NUCLEIC A"},{"key":"e_1_2_1_2_1","first-page":"105","volume":"2","year":"1984","unstructured":"BERNSTEIN, R.M., MOL BIOL MED 2: 105 (1984).","journal-title":"MOL BIOL MED"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1084\/jem.163.5.1281","volume":"163","year":"1986","unstructured":"BUNN, C.C., AUTOANTIBODIES AGAINST ALANYL-TRANSFER RNA-SYNTHETASE AND TRANSFER RNAALA COEXIST AND ARE ASSOCIATED WITH MYOSITIS, JOURNAL OF EXPERIMENTAL MEDICINE 163: 1281 (1986).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_4_1","first-page":"21","volume":"4","year":"1987","unstructured":"BUNN, C.C., 2 HUMAN TRANSFER RNA(ALA) FAMILIES ARE RECOGNIZED BY AUTOANTIBODIES IN POLYMYOSITIS SERA, MOLECULAR BIOLOGY & MEDICINE 4: 21 (1987).","journal-title":"MOLECULAR BIOLOGY & MEDICINE"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/0161-5890(82)90360-1","volume":"19","year":"1982","unstructured":"EILAT, D, MONOCLONAL AUTOANTIBODIES - AN APPROACH TO STUDYING AUTOIMMUNE-DISEASE, MOLECULAR IMMUNOLOGY 19: 943 (1982).","journal-title":"MOLECULAR IMMUNOLOGY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/259141a0","volume":"259","year":"1976","unstructured":"EILAT, D, ANTIBODIES TO NATIVE TRANSFER-RNA IN NZB-NZW MICE, NATURE 259: 141 (1976).","journal-title":"NATURE"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/0006-291X(82)91756-9","volume":"109","year":"1982","unstructured":"EPSTEIN, P, IDENTIFICATION OF 3 DIFFERENT ANTI-4S RNA SERA ASSOCIATED WITH AUTOIMMUNE-DISEASE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 109: 548 (1982).","journal-title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"},{"key":"e_1_2_1_8_1","first-page":"2800","volume":"71","year":"1974","unstructured":"FUCHS, S, ANTIBODIES TO YEAST PHENYLALANINE TRANSFER RIBONUCLEIC-ACID ARE SPECIFIC FOR ODD NUCLEOSIDE Y IN ANTICODON LOOP, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 2800 (1974).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3823878"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1172\/JCI110587","volume":"70","year":"1982","unstructured":"HARDIN, J.A., ANTIBODIES FROM PATIENTS WITH CONNECTIVE-TISSUE DISEASES BIND SPECIFIC SUBSETS OF CELLULAR RNA-PROTEIN PARTICLES, JOURNAL OF CLINICAL INVESTIGATION 70: 141 (1982).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_11_1","first-page":"4970","volume":"71","year":"1974","unstructured":"KIM, S.H., GENERAL STRUCTURE OF TRANSFER-RNA MOLECULES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 4970 (1974).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1084\/jem.160.2.420","volume":"160","year":"1984","unstructured":"MATHEWS, M.B., ANTI-THREONYL-TRNA SYNTHETASE, A 2ND MYOSITIS-RELATED AUTOANTIBODY, JOURNAL OF EXPERIMENTAL MEDICINE 160: 420 (1984).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/304177a0","volume":"304","year":"1983","unstructured":"MATHEWS, M.B., MYOSITIS AUTOANTIBODY INHIBITS HISTIDYL-TRANSFER RNA-SYNTHETASE - A MODEL FOR AUTOIMMUNITY, NATURE 304: 177 (1983).","journal-title":"NATURE"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1021\/bi00584a025","volume":"18","year":"1979","unstructured":"MUNNS, T.W., ANTIBODY NUCLEIC ACID COMPLEXES - INHIBITION OF TRANSLATION OF SILKMOTH CHORION MESSENGER RIBONUCLEIC-ACID WITH ANTIBODIES SPECIFIC FOR 7-METHYLGUANOSINE, BIOCHEMISTRY 18: 3804 (1979).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_15_1","first-page":"824","volume":"2","year":"1983","unstructured":"PLOTZ, P.H., AUTOANTIBODIES ARE ANTI-IDIOTYPE ANTIBODIES TO ANTIVIRAL ANTIBODIES, LANCET 2: 824 (1983).","journal-title":"LANCET"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1146\/annurev.bb.12.060183.002355","volume":"12","year":"1983","unstructured":"RIGLER, R, DYNAMICS OF TRANSFER-RNA, ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING 12: 475 (1983).","journal-title":"ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING"},{"key":"e_1_2_1_17_1","first-page":"187","volume":"49","year":"1979","unstructured":"SCHIMMEL, P.R., ADVANCES IN ENZYMOLOGY 49: 187 (1979).","journal-title":"ADVANCES IN ENZYMOLOGY"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1172\/JCI111704","volume":"75","year":"1985","unstructured":"SCHWARTZ, R.S., ORIGINS OF ANTI-DNA AUTOANTIBODIES, JOURNAL OF CLINICAL INVESTIGATION 75: 321 (1985).","journal-title":"JOURNAL OF CLINICAL INVESTIGATION"},{"key":"e_1_2_1_19_1","first-page":"71","volume":"70","year":"1980","unstructured":"STOLLAR, B.D., METHODS IN ENZYMOLOGY 70: 71 (1980).","journal-title":"METHODS IN ENZYMOLOGY"},{"key":"e_1_2_1_20_1","first-page":"4469","volume":"83","year":"1986","unstructured":"STOLLAR, B.D., A RECOGNITION SITE ON SYNTHETIC HELICAL OLIGONUCLEOTIDES FOR MONOCLONAL ANTI-NATIVE DNA AUTOANTIBODY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 4469 (1986).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/10409238609115899","volume":"20","year":"1986","unstructured":"STOLLAR, D, CRC CRIT REV BIOCH 20: 1 (1986).","journal-title":"CRC CRIT REV BIOCH"},{"key":"e_1_2_1_22_1","first-page":"874","volume":"48","year":"1962","unstructured":"STOLLAR, D, ANTIGENIC DETERMINANTS OF DENATURED DNA REACTIVE WITH LUPUS ERYTHEMATOSUS SERUM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 48: 874 (1962).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0065-2776(08)60836-6","volume":"33","year":"1982","unstructured":"TAN, E.M., AUTOANTIBODIES TO NUCLEAR ANTIGENS (ANA) - THEIR IMMUNOBIOLOGY AND MEDICINE, ADVANCES IN IMMUNOLOGY 33: 167 (1982).","journal-title":"ADVANCES IN IMMUNOLOGY"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"5467","DOI":"10.1016\/S0021-9258(19)57239-8","volume":"261","year":"1986","unstructured":"WILUSZ, J, AUTOANTIBODIES SPECIFIC FOR U1 RNA AND INITIATOR METHIONINE TRANSFER-RNA, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 5467 (1986).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.2446387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T15:42:16Z","timestamp":1705074136000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.2446387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,11,20]]},"references-count":24,"journal-issue":{"issue":"4830","published-print":{"date-parts":[[1987,11,20]]}},"alternative-id":["10.1126\/science.2446387"],"URL":"https:\/\/doi.org\/10.1126\/science.2446387","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,11,20]]}}}