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By means of RAWTS, it was possible to sequence each of four tissue-specific human messenger RNAs (blue pigment, factor IX, phenylalanine hydroxylase, and tyrosine hydroxylase) in four cell types examined (white blood cells, liver, K562 erythroleukemia cells, and chorionic villus cells). These results indicate that there is a basal rate of transcription, splicing, and polyadenylation of tissue-specific mRNAs in adult and embryonic tissues. In addition to revealing sequence information, it is possible to generate a desired in vitro translation product by incorporating a translation initiation signal into the appropriate PCR primer. RAWTS can be used to obtain novel mRNA sequence information from other species as illustrated with a segment of the catalytic domain of factor IX. In general, the ability to obtain mRNA sequences rapidly across species boundaries should aid both the study of protein evolution and the identification of sequences crucial for protein structure and function.<\/jats:p>","DOI":"10.1126\/science.2565599","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T21:21:28Z","timestamp":1160083288000},"page":"331-334","source":"Crossref","is-referenced-by-count":223,"title":["Access to a Messenger RNA Sequence or Its Protein Product Is Not Limited by Tissue or Species Specificity"],"prefix":"10.1126","volume":"244","author":[{"given":"Gobinda","family":"Sarkar","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Biology, Mayo Clinic\/Foundation, Rochester, MN 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve S.","family":"Sommer","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Biology, Mayo Clinic\/Foundation, Rochester, MN 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1021\/bi00352a001","volume":"25","year":"1986","unstructured":"DILELLA, A.G., MOLECULAR-STRUCTURE AND POLYMORPHIC MAP OF THE HUMAN PHENYLALANINE-HYDROXYLASE GENE, BIOCHEMISTRY 25: 743 (1986).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_2_1","first-page":"544","volume":"85","year":"1988","unstructured":"ENGELKE, D.R., DIRECT SEQUENCING OF ENZYMATICALLY AMPLIFIED HUMAN GENOMIC DNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 544 (1988).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/326707a0","volume":"326","year":"1987","unstructured":"GRIMA, B, A SINGLE HUMAN-GENE ENCODING MULTIPLE TYROSINE HYDROXYLASES WITH DIFFERENT PREDICTED FUNCTIONAL-CHARACTERISTICS, NATURE 326: 707 (1987).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","first-page":"4990","volume":"76","year":"1979","unstructured":"KATAYAMA, K, COMPARISON OF AMINO-ACID SEQUENCE OF BOVINE COAGULATION FACTOR-IX (CHRISTMAS FACTOR) WITH THAT OF OTHER VITAMIN-K-DEPENDENT PLASMA-PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 4990 (1979).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_5_1","unstructured":"KOEBERL D.D. unpublished data."},{"key":"e_1_2_1_6_1","first-page":"2737","volume":"8","year":"1988","unstructured":"KOZAK, M, LEADER LENGTH AND SECONDARY STRUCTURE MODULATE MESSENGER-RNA FUNCTION UNDER CONDITIONS OF STRESS, MOLECULAR AND CELLULAR BIOLOGY 8: 2737 (1988).","journal-title":"MOLECULAR AND CELLULAR BIOLOGY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1021\/bi00324a002","volume":"24","year":"1985","unstructured":"KWOK, SCM, NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH COMPLEMENTARY-DNA CLONE AND AMINO-ACID SEQUENCE OF HUMAN PHENYLALANINE-HYDROXYLASE, BIOCHEMISTRY 24: 556 (1985).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1007\/BF02603118","volume":"25","year":"1987","unstructured":"LI, W.H., AN EVALUATION OF THE MOLECULAR CLOCK HYPOTHESIS USING MAMMALIAN DNA-SEQUENCES, JOURNAL OF MOLECULAR EVOLUTION 25: 330 (1987).","journal-title":"JOURNAL OF MOLECULAR EVOLUTION"},{"key":"e_1_2_1_9_1","unstructured":"Maniatis T. Molecular Cloning: A Laboratory Manual (1982)."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2937147"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"6910","DOI":"10.1021\/bi00396a007","volume":"26","year":"1987","unstructured":"OMALLEY, K.L., ISOLATION AND CHARACTERIZATION OF THE HUMAN TYROSINE-HYDROXYLASE GENE - IDENTIFICATION OF 5' ALTERNATIVE SPLICE SITES RESPONSIBLE FOR MULTIPLE MESSENGER-RNAS, BIOCHEMISTRY 26: 6910 (1987).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/0092-8674(85)90200-4","volume":"40","year":"1985","unstructured":"PELLETIER, J, INSERTION MUTAGENESIS TO INCREASE SECONDARY STRUCTURE WITHIN THE 5' NONCODING REGION OF A EUKARYOTIC MESSENGER-RNA REDUCES TRANSLATIONAL EFFICIENCY, CELL 40: 515 (1985).","journal-title":"CELL"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/316643a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448875"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"5197","DOI":"10.1093\/nar\/16.11.5197","volume":"16","year":"1988","unstructured":"SARKAR, G, RNA AMPLIFICATION WITH TRANSCRIPT SEQUENCING (RAWTS), NUCLEIC ACIDS RESEARCH 16: 5197 (1988).","journal-title":"NUCLEIC ACIDS RESEARCH"},{"key":"e_1_2_1_16_1","unstructured":"SARKAR G unpublished data."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3340835"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/330384a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1021\/bi00335a049","volume":"24","year":"1985","unstructured":"YOSHITAKE, S, NUCLEOTIDE-SEQUENCE OF THE GENE FOR HUMAN FACTOR-IX (ANTIHEMOPHILIC FACTOR-B), BIOCHEMISTRY 24: 3736 (1985).","journal-title":"BIOCHEMISTRY"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.2565599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T22:38:57Z","timestamp":1705099137000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.2565599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4,21]]},"references-count":19,"journal-issue":{"issue":"4902","published-print":{"date-parts":[[1989,4,21]]}},"alternative-id":["10.1126\/science.2565599"],"URL":"https:\/\/doi.org\/10.1126\/science.2565599","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4,21]]}}}