{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:58:24Z","timestamp":1760345904565},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4700","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1985,5,10]]},"abstract":"<jats:p>\n            The obligate intracellular prokaryote\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            is the etiological agent of trachoma and is a primary causative pathogen of sexually transmitted genital tract disease; both diseases affect millions of people each year. The cloning of genes encoding the enzyme or enzymes producing the genus-specific lipopolysaccharide antigen of\n            <jats:italic>Chlamydia<\/jats:italic>\n            into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            is reported here. The cloned chlamydial lipopolysaccharide antigen appears to be a hybrid lipopolysaccharide molecule composed of both\n            <jats:italic>Chlamydia<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            components. The chlamydial lipopolysaccharide antigen is expressed on the surfaces of the viable\n            <jats:italic>Escherichia coli<\/jats:italic>\n            recombinants. These findings may have a significant impact on defining the role of this highly conserved antigen in the pathogenesis and diagnosis of chlamydial infections.\n          <\/jats:p>","DOI":"10.1126\/science.2581315","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T19:42:50Z","timestamp":1160077370000},"page":"742-744","source":"Crossref","is-referenced-by-count":61,"title":["Expression of the Chlamydial Genus-Specific Lipopolysaccharide Epitope in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1126","volume":"228","author":[{"given":"Francis E.","family":"Nano","sequence":"first","affiliation":[{"name":"Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harlan D.","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1128\/iai.19.2.598-606.1978","volume":"19","year":"1978","unstructured":"BYRNE, G.I., PARASITE-SPECIFIED PHAGOCYTOSIS OF CHLAMYDIA-PSITTACI AND CHLAMYDIA-TRACHOMATIS BY L-CELLS AND HELA-CELLS, INFECTION AND IMMUNITY 19: 598 (1978).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/iai.44.2.306-314.1984","volume":"44","year":"1984","unstructured":"CALDWELL, D.H., INFECT IMMUN 44: 306 (1984).","journal-title":"INFECT IMMUN"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4049\/jimmunol.115.4.963","volume":"115","year":"1975","unstructured":"CALDWELL, H.D., ANTIGENIC ANALYSIS OF CHLAMYDIAE BY 2-DIMENSIONAL IMMUNOELECTROPHORESIS .1. ANTIGENIC HETEROGENEITY BETWEEN CHLAMYDIA-TRACHOMATIS AND CHLAMYDIA-PSITTACI, JOURNAL OF IMMUNOLOGY 115: 963 (1975).","journal-title":"JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/S0021-9258(17)37876-6","volume":"254","year":"1979","unstructured":"CREEGER, E.S., CLONING OF GENES FOR BACTERIAL GLYCOSYLTRANSFERASES .1. SELECTION OF HYBRID PLASMIDS CARRYING GENES FOR 2 GLUCOSYLTRANSFERASES, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 804 (1979).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1093\/clinids\/7.6.768","volume":"7","year":"1985","unstructured":"Dawson, C. R., Reviews of Infectious Diseases. 7: 768 (1985).","journal-title":"Reviews of Infectious Diseases"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/physci241020a0","volume":"241","year":"1973","unstructured":"FRENS, G, CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS, NATURE-PHYSICAL SCIENCE 241: 20 (1973).","journal-title":"NATURE-PHYSICAL SCIENCE"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1128\/jb.110.2.706-721.1972","volume":"110","year":"1972","unstructured":"FRIIS, R.R., INTERACTION OF L CELLS AND CHLAMYDIA-PSITTACI - ENTRY OF PARASITE AND HOST RESPONSES TO ITS DEVELOPMENT, JOURNAL OF BACTERIOLOGY 110: 706 (1972).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/infdis\/132.1.87","volume":"132","year":"1975","unstructured":"GRAYSTON, J.T., NEW KNOWLEDGE OF CHLAMYDIAE AND DISEASES THEY CAUSE, JOURNAL OF INFECTIOUS DISEASES 132: 87 (1975).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.193184"},{"key":"e_1_2_1_10_1","first-page":"31","volume":"80","year":"1983","unstructured":"HELFMAN, D.M., IDENTIFICATION OF CLONES THAT ENCODE CHICKEN TROPOMYOSIN BY DIRECT IMMUNOLOGICAL SCREENING OF A CDNA EXPRESSION LIBRARY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80: 31 (1983).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1128\/jb.161.1.277-284.1985","volume":"161","year":"1985","unstructured":"KADAM, S.K., CLONING OF RFAG-GENES, B-GENES, I-GENES AND J-GENES FOR GLYCOSYLTRANSFERASE ENZYMES FOR SYNTHESIS OF THE LIPOPOLYSACCHARIDE CORE OF SALMONELLA-TYPHIMURIUM, JOURNAL OF BACTERIOLOGY 161: 277 (1985).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1128\/jb.96.4.1421-1423.1968","volume":"96","year":"1968","unstructured":"KINGSBURY, D.T., LACK OF DEOXYRIBONUCLEIC ACID HOMOLOGY BETWEEN SPECIES OF GENUS CHLAMYDIA, JOURNAL OF BACTERIOLOGY 96: 1421 (1968).","journal-title":"JOURNAL OF BACTERIOLOGY"},{"key":"e_1_2_1_13_1","first-page":"219","volume":"31","year":"1981","unstructured":"MAKELA, P.H., SYMPOSIUM OF THE SOCIETY FOR GENERAL MICROBIOLOGY 31: 219 (1981).","journal-title":"SYMPOSIUM OF THE SOCIETY FOR GENERAL MICROBIOLOGY"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1128\/iai.44.3.609-613.1984","volume":"44","year":"1984","unstructured":"NURMINEN, M, IMMUNOLOGICALLY RELATED KETODEOXYOCTONATE-CONTAINING STRUCTURES IN CHLAMYDIA-TRACHOMATIS, RE MUTANTS OF SALMONELLA SPECIES, AND ACINNETOBACTER-CALCOACETICUS VAR ANITRATUS, INFECTION AND IMMUNITY 44: 609 (1984).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6344216"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.2476\/asjaa.25.23","volume":"25","year":"1973","unstructured":"OSAKI, H, ACTA ARACHNOLOGIA 25: 23 (1973).","journal-title":"ACTA ARACHNOLOGIA"},{"key":"e_1_2_1_17_1","first-page":"15","year":"1979","unstructured":"OSBORN, M.J., BACTERIAL OUTER MEMB: 15 (1979).","journal-title":"BACTERIAL OUTER MEMB"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1128\/iai.46.2.361-366.1984","volume":"46","year":"1984","unstructured":"ROBINSON, E.N., ULTRASTRUCTURAL-LOCALIZATION OF SPECIFIC GONOCOCCAL MACROMOLECULES WITH ANTIBODY-GOLD SPHERE IMMUNOLOGICAL PROBES, INFECTION AND IMMUNITY 46: 361 (1984).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev.mi.34.100180.001441","volume":"34","year":"1980","unstructured":"SCHACHTER, J, CHLAMYDIAE, ANNUAL REVIEW OF MICROBIOLOGY 34: 285 (1980).","journal-title":"ANNUAL REVIEW OF MICROBIOLOGY"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1056\/NEJM197802232980805","volume":"298","year":"1978","unstructured":"SCHACHTER, J, CHLAMYDIAL INFECTIONS .1., NEW ENGLAND JOURNAL OF MEDICINE 298: 428 (1978).","journal-title":"NEW ENGLAND JOURNAL OF MEDICINE"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/0378-1119(82)90015-4","volume":"19","year":"1982","unstructured":"VIEIRA, J, THE PUC PLASMIDS, AN M13MP7-DERIVED SYSTEM FOR INSERTION MUTAGENESIS AND SEQUENCING WITH SYNTHETIC UNIVERSAL PRIMERS, GENE 19: 259 (1982).","journal-title":"GENE"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1128\/iai.46.1.55-63.1984","volume":"46","year":"1984","unstructured":"WATANABE, H, SMALL VIRULENCE PLASMID OF SHIGELLA-DYSENTERIAE-1 STRAIN-W30864 ENCODES A 41,000-DALTON PROTEIN INVOLVED IN FORMATION OF SPECIFIC LIPOPOLYSACCHARIDE SIDE-CHAINS OF SEROTYPE-1 ISOLATES, INFECTION AND IMMUNITY 46: 55 (1984).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_23_1","first-page":"83","volume":"5","year":"1965","unstructured":"WESTPHAL, O, METHODS CARBOHYDRATE 5: 83 (1965).","journal-title":"METHODS CARBOHYDRATE"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.2581315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T22:13:18Z","timestamp":1705097598000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.2581315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,5,10]]},"references-count":23,"journal-issue":{"issue":"4700","published-print":{"date-parts":[[1985,5,10]]}},"alternative-id":["10.1126\/science.2581315"],"URL":"https:\/\/doi.org\/10.1126\/science.2581315","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,5,10]]}}}