{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:43:36Z","timestamp":1791067416820,"version":"4.1.0"},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5245","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1996,1,5]]},"abstract":"<jats:p>\n            Developing insects repeatedly shed their cuticle by means of a stereotyped behavior called ecdysis, thought to be initiated by the brain peptide eclosion hormone. Here an ecdysis-triggering hormone, Mas-ETH, is described from the tobacco hornworm\n            <jats:italic>Manduca sexta<\/jats:italic>\n            . Mas-ETH contains 26 amino acids and is produced by a segmentally distributed endocrine system of epitracheal glands (EGs). The EGs undergo a marked reduction in volume, appearance, and immunohistochemical staining during ecdysis, at which time Mas-ETH is found in the hemolymph. Injection of EGs extract or synthetic Mas-ETH into pharate larvae, pupae, or adults initiates preecdysis within 2 to 10 minutes, followed by ecdysis. Sensitivity to injected Mas-ETH appears much earlier before ecdysis and occurs with shorter latency than that reported for eclosion hormone. The isolated central nervous system responds to Mas-ETH, but not to eclosion hormone, with patterned motor bursting corresponding to in vivo preecdysis and ecdysis. Mas-ETH may be an immediate blood-borne trigger for ecdysis through a direct action on the nervous system.\n          <\/jats:p>","DOI":"10.1126\/science.271.5245.88","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T18:30:41Z","timestamp":1161973841000},"page":"88-91","source":"Crossref","is-referenced-by-count":223,"title":["Identification of Ecdysis-Triggering Hormone from an Epitracheal Endocrine System"],"prefix":"10.1126","volume":"271","author":[{"given":"Du\u0161an","family":"\u017dit\u0148an","sequence":"first","affiliation":[{"name":"D. \u017dit\u0148an, J. L. Hermesman, M. E. Adams, Department of Entomology and Agricultural Experiment Station, 5419 Boyce Hall, University of California, Riverside, CA 92521, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy G.","family":"Kingan","sequence":"additional","affiliation":[{"name":"T. G. Kingan, U.S. Department of Agriculture ARS Insect Neurobiology and Hormone Laboratory, BARC-East Building 306, Room 322, Beltsville, MD 20705, USA, and Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21228, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L.","family":"Hermesman","sequence":"additional","affiliation":[{"name":"D. \u017dit\u0148an, J. L. Hermesman, M. E. Adams, Department of Entomology and Agricultural Experiment Station, 5419 Boyce Hall, University of California, Riverside, CA 92521, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Adams","sequence":"additional","affiliation":[{"name":"D. \u017dit\u0148an, J. L. Hermesman, M. E. Adams, Department of Entomology and Agricultural Experiment Station, 5419 Boyce Hall, University of California, Riverside, CA 92521, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1508\/cytologia.57.195","volume":"57","author":"AKAI H","year":"1992","unstructured":"AKAI H, CYTOLOGIA 57, 195 (1992).","journal-title":"CYTOLOGIA"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/0022-1910(82)90148-2","article-title":"THE ROLE OF ECLOSION HORMONE IN THE LARVAL ECDYSES OF MANDUCA-SEXTA","volume":"28","author":"COPENHAVER P.F.","year":"1982","unstructured":"COPENHAVER P.F., THE ROLE OF ECLOSION HORMONE IN THE LARVAL ECDYSES OF MANDUCA-SEXTA, JOURNAL OF INSECT PHYSIOLOGY 28, 695 (1982).","journal-title":"JOURNAL OF INSECT PHYSIOLOGY"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/0022-1910(84)90043-X","article-title":"ECDYSTEROID REGULATION OF THE ONSET OF CUTICULAR MELANIZATION IN ALLATECTOMIZED AND BLACK MUTANT MANDUCA-SEXTA LARVAE","volume":"30","author":"CURTIS A.T.","year":"1984","unstructured":"CURTIS A.T., ECDYSTEROID REGULATION OF THE ONSET OF CUTICULAR MELANIZATION IN ALLATECTOMIZED AND BLACK MUTANT MANDUCA-SEXTA LARVAE, JOURNAL OF INSECT PHYSIOLOGY 30, 597 (1984).","journal-title":"JOURNAL OF INSECT PHYSIOLOGY"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"7704","DOI":"10.1523\/JNEUROSCI.14-12-07704.1994","article-title":"NEUROPEPTIDE INDUCTION OF CYCLIC-GMP INCREASES IN THE INSECT CNS - RESOLUTION AT THE LEVEL OF SINGLE IDENTIFIABLE NEURONS","volume":"14","author":"EWER J","year":"1994","unstructured":"EWER J, NEUROPEPTIDE INDUCTION OF CYCLIC-GMP INCREASES IN THE INSECT CNS - RESOLUTION AT THE LEVEL OF SINGLE IDENTIFIABLE NEURONS, JOURNAL OF NEUROSCIENCE 14, 7704 (1994).","journal-title":"JOURNAL OF NEUROSCIENCE"},{"key":"e_1_2_1_6_2","first-page":"697","article-title":"THE ROLES OF CENTRAL AND PERIPHERAL ECLOSION HORMONE-RELEASE IN THE CONTROL OF ECDYSIS BEHAVIOR IN MANDUCA-SEXTA","volume":"168","author":"HEWES R.S.","year":"1991","unstructured":"HEWES R.S., THE ROLES OF CENTRAL AND PERIPHERAL ECLOSION HORMONE-RELEASE IN THE CONTROL OF ECDYSIS BEHAVIOR IN MANDUCA-SEXTA, JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY 168, 697 (1991).","journal-title":"JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.1073\/pnas.79.8.2700","article-title":"ANTIBODIES TO HORSERADISH-PEROXIDASE AS SPECIFIC NEURONAL MARKERS IN DROSOPHILA AND IN GRASSHOPPER EMBRYOS","volume":"79","author":"JAN L.Y.","year":"1982","unstructured":"JAN L.Y., ANTIBODIES TO HORSERADISH-PEROXIDASE AS SPECIFIC NEURONAL MARKERS IN DROSOPHILA AND IN GRASSHOPPER EMBRYOS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79, 2700 (1982).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182000011975","volume":"36","author":"KEILIN D","year":"1944","unstructured":"KEILIN D, PARASITOLOGY 36, 1 (1944).","journal-title":"PARASITOLOGY"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1002\/cne.902730406","article-title":"MONOCLONAL-ANTIBODIES TO THE MOLLUSCAN SMALL CARDIOACTIVE PEPTIDE SCPB - 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