{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:21:16Z","timestamp":1791314476841,"version":"4.3.1"},"reference-count":30,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5341","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1997,11,14]]},"abstract":"<jats:p>\n            The Janus family of tyrosine kinases (JAK) plays an essential role in development and in coupling cytokine receptors to downstream intracellular signaling events. A t(9;12)(p24;p13) chromosomal translocation in a T cell childhood acute lymphoblastic leukemia patient was characterized and shown to fuse the 3\u2032 portion of\n            <jats:italic>JAK2<\/jats:italic>\n            to the 5\u2032 region of\n            <jats:italic>TEL<\/jats:italic>\n            , a gene encoding a member of the ETS transcription factor family. The TEL-JAK2 fusion protein includes the catalytic domain of JAK2 and the TEL-specific oligomerization domain. TEL-induced oligomerization of TEL-JAK2 resulted in the constitutive activation of its tyrosine kinase activity and conferred cytokine-independent proliferation to the interleukin-3\u2013dependent Ba\/F3 hematopoietic cell line.\n          <\/jats:p>","DOI":"10.1126\/science.278.5341.1309","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:45:15Z","timestamp":1027763115000},"page":"1309-1312","source":"Crossref","is-referenced-by-count":680,"title":["A TEL-JAK2 Fusion Protein with Constitutive Kinase Activity in Human Leukemia"],"prefix":"10.1126","volume":"278","author":[{"given":"Virginie","family":"Lacronique","sequence":"first","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Boureux","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ve\u0301ronique","family":"Della Valle","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u0301le\u0300ne","family":"Poirel","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine Tran","family":"Quang","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. 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Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Berthou","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. 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Bernard, U 301 de l'Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale and SD 401 No. 301 CNRS, Institut de Ge\u0301ne\u0301tique Mole\u0301culaire, 27 rue Juliette Dodu, 75010 Paris, France."},{"name":"A. Boureux, C. Tran Quang, J. Ghysdael, CNRS Unite\u0301 Mixte de Recherche 146, Institut Curie\u2013Section de Recherche, Centre Universitaire, 91405 Orsay Cedex, France."},{"name":"C. Berthou and M. Lessard, Unite\u0301 d'He\u0301matologie, Laboratoire de Cytoge\u0301ne\u0301tique, Ho\u0302pital Morvan, Centre Hospitalier Re\u0301gional de Brest, 29609 Brest Cedex, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Ghysdael","sequence":"additional","affiliation":[{"name":"V. Lacronique, V. Della Valle, H. Poirel, M. Mauchauffe\u0301, R. Berger, O. A. 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H., Nature 371, 143 (1994).","journal-title":"Nature"},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3109\/10428199609054854","volume":"23","author":"Bernard O. A.","year":"1996","unstructured":"Bernard O. A., Romana S. P., Poirel H., Berger R., Leuk. Lymphoma 23, 459 (1996).","journal-title":"Leuk. Lymphoma"},{"key":"e_1_3_1_4_2","first-page":"7","volume":"1288","author":"Golub T.","year":"1996","unstructured":"Golub T., et al., Biochim. Biophys. Acta 1288, 7 (1996).","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0092-8674(94)90322-0","volume":"77","author":"Golub T. R.","year":"1994","unstructured":"Golub T. R., Barker G. F., Lovett M., Gilliland D. G., Cell 77, 307 (1994).","journal-title":"Cell"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1182\/blood.V85.12.3662.bloodjournal85123662","volume":"85","author":"Romana S. 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Biol."},{"key":"e_1_3_1_21_2","unstructured":"G418-resistant Ba\/F3 transfectants expressing the indicated proteins and control cells were grown in RPMI 1640 medium containing 10% fetal bovine serum and 2% WEHI conditioned medium as a source of IL-3. Cells were washed twice in the same IL-3\u2013free medium and then seeded in IL-3\u2013free medium in 96-well plates at a concentration of 5 \u00d7 10 4 cells\/ml (10 4 cells per well). The number of wells showing proliferating cells in either the absence or presence of IL-3 was scored after 1 week in culture."},{"key":"e_1_3_1_22_2","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/379645a0","volume":"379","author":"Meydan N.","year":"1996","unstructured":"Meydan N., et al., Nature 379, 645 (1996).","journal-title":"Nature"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07127.x"},{"key":"e_1_3_1_24_2","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1128\/MCB.17.3.1562","volume":"17","author":"Luo H.","year":"1997","unstructured":"Luo H., et al., Mol. Cell. Biol. 17, 1562 (1997).","journal-title":"Mol. Cell. Biol."},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07285.x"},{"key":"e_1_3_1_26_2","doi-asserted-by":"crossref","first-page":"4263","DOI":"10.1182\/blood.V86.11.4263.bloodjournal86114263","volume":"86","author":"Romana S. P.","year":"1995","unstructured":"Romana S. P., et al., Blood 86, 4263 (1995).","journal-title":"Blood"},{"key":"e_1_3_1_27_2","unstructured":"The cDNA from patient 2 (an atypical chronic myelogenous leukemia case in transformation) was amplified with the TEL primer ATGCACCCTCTGATCCTGAACC and the JAK2 primer TGGTGAGGTTGGTACATCAG. The cDNA from patient 3 (a precursor B cell ALL case) was amplified with the TEL primer TTCCACCCTGGAAACTCTATA and the JAK2 primer AAGGTTTGCTAATTCTGCCCACTTTGGTGC. PCR products were sequenced on an ALF sequencer."},{"key":"e_1_3_1_28_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/sj.onc.1200829","volume":"14","author":"Poirel H.","year":"1997","unstructured":"Poirel H., et al., Oncogene 14, 349 (1997).","journal-title":"Oncogene"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.12.3587"},{"key":"e_1_3_1_30_2","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1002\/j.1460-2075.1995.tb07284.x","volume":"14","author":"Pallard C.","year":"1995","unstructured":"Pallard C., et al., EMBO J. 14, 2847 (1995).","journal-title":"EMBO J."},{"key":"e_1_3_1_31_2","unstructured":"We thank M. Le Coniat for FISH experiments H. Beug for the murine Jak2 cDNA P. Mayeux for helpful hints P. Peeters and P. Marynen for sharing unpublished data and S. Gisselbrecht and G. Calothy for critical reading of the manuscript. Supported by the Association pour la Recherche sur le Cancer (V.L. and A.B.) the Ligue Nationale Contre le Cancer (C.T.Q.) and funds from INSERM CNRS Institut Curie Association pour la Recherche sur le Cancer Ligue Nationale Contre le Cancer Ligue D\u00e9partementale Contre le Cancer (Comit\u00e9 de Paris) and the Biomed Programme of the European Community (contract BMH4-CT96-1355)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.278.5341.1309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T04:31:07Z","timestamp":1705120267000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.278.5341.1309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11,14]]},"references-count":30,"journal-issue":{"issue":"5341","published-print":{"date-parts":[[1997,11,14]]}},"alternative-id":["10.1126\/science.278.5341.1309"],"URL":"https:\/\/doi.org\/10.1126\/science.278.5341.1309","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11,14]]}}}