{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:44:25Z","timestamp":1791186265602,"version":"4.1.0"},"reference-count":32,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5391","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1998,11,6]]},"abstract":"<jats:p>\n                    To test the hypothesis that auxin-binding protein 1 (ABP1) is a receptor controlling auxin-mediated plant cell expansion,\n                    <jats:italic>ABP1<\/jats:italic>\n                    complementary DNAs were expressed in a controllable fashion in tobacco plants and constitutively in maize cell lines. Induction of\n                    <jats:italic>Arabidopsis ABP1<\/jats:italic>\n                    expression in tobacco leaf strips resulted in an increased capacity for auxin-mediated cell expansion, whereas induction of ABP1 in intact plants resulted in leaves with a normal morphology, but larger cells. Similarly, constitutive expression of maize\n                    <jats:italic>ABP1<\/jats:italic>\n                    in maize cell lines conferred on them the capacity to respond to auxin by increasing cell size. These results support a role of ABP1 as an auxin receptor controlling plant growth.\n                  <\/jats:p>","DOI":"10.1126\/science.282.5391.1114","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:50:14Z","timestamp":1027749014000},"page":"1114-1117","source":"Crossref","is-referenced-by-count":227,"title":["Auxin-Dependent Cell Expansion Mediated by Overexpressed Auxin-Binding Protein 1"],"prefix":"10.1126","volume":"282","author":[{"given":"Alan M.","family":"Jones","sequence":"first","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Hoam","family":"Im","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Savka","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Gregory","family":"DeWitt","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Shillito","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew N.","family":"Binns","sequence":"additional","affiliation":[{"name":"A. M. Jones, K.-H. Im, M.-J. Wu, N. G. DeWitt, Department of Biology, University of North Carolina, Chapel Hill, NC 27599\u20133280, USA. M. A. Savka and A. N. Binns, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104\u20136018, USA. R. Shillito, Agricultural Research Center, AgrEvo USA, Pikeville, NC 27863, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1146\/annurev.pp.45.060194.002141","volume":"45","author":"Jones A. M.","year":"1994","unstructured":"Jones A. M., Annu. Rev. Plant Physiol. Plant Mol. Biol. 45, 393 (1994);","journal-title":"Annu. Rev. Plant Physiol. Plant Mol. Biol."},{"key":"e_1_3_1_2_3","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1093\/jxb\/46.12.1787","volume":"46","author":"Napier R. M.","year":"1995","unstructured":"Napier R. M., J. Exp. Bot. 46, 1787 (1995).","journal-title":"J. Exp. Bot."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1104\/pp.111.1.9","volume":"111","author":"Abel S.","year":"1996","unstructured":"Abel S., Theologis A., Plant Physiol. 111, 9 (1996);","journal-title":"Plant Physiol."},{"key":"e_1_3_1_3_3","first-page":"18","volume":"11","author":"Ray P. M.","year":"1992","unstructured":"Ray P. M., Curr. Top. Plant Biochem. Physiol. 11, 18 (1992);","journal-title":"Curr. Top. Plant Biochem. Physiol."},{"key":"e_1_3_1_3_4","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1073\/pnas.84.9.2747","volume":"84","author":"Kutschera U.","year":"1987","unstructured":"Kutschera U., Briggs W. R., Proc. Natl. Acad. Sci. U.S.A. 84, 2747 (1987).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"21136","DOI":"10.1016\/S0021-9258(17)31940-3","volume":"269","author":"Brown J. C.","year":"1994","unstructured":"Brown J. C., Jones A. M., J. Biol. Chem. 269, 21136 (1994);","journal-title":"J. Biol. Chem."},{"key":"e_1_3_1_4_3","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/S0031-9422(00)94807-6","volume":"35","author":"Edgerton M. D.","year":"1994","unstructured":"Edgerton M. D., Tropsha A., Jones A. M., Phytochemistry 35, 1111 (1994);","journal-title":"Phytochemistry"},{"key":"e_1_3_1_4_4","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1104\/pp.60.4.585","volume":"60","author":"Ray P. M.","year":"1977","unstructured":"Ray P. M., Dohrmann U., Hertel R., Plant Physiol. 60, 585 (1977) ;","journal-title":"Plant Physiol."},{"key":"e_1_3_1_4_5","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/oxfordjournals.jbchem.a135621","volume":"99","author":"Shimomura S.","year":"1986","unstructured":"Shimomura S., Sotobayashi S., Futai M., Fukui T., J. Biochem. 99, 1513 (1986);","journal-title":"J. Biochem."},{"key":"e_1_3_1_4_6","doi-asserted-by":"crossref","first-page":"6153","DOI":"10.1073\/pnas.86.16.6153","volume":"86","author":"Jones A. M.","year":"1989","unstructured":"Jones A. M., Venis M. A., Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF00203122","volume":"14","author":"Harnden D.","year":"1995","unstructured":"Harnden D., Jones A. M., J. Plant Growth Reg. 14, 109 (1995).","journal-title":"J. Plant Growth Reg."},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1104\/pp.101.2.595","volume":"101","author":"Jones A. M.","year":"1993","unstructured":"Jones A. M., Herman E., Plant Physiol. 101, 595 (1993);","journal-title":"Plant Physiol."},{"key":"e_1_3_1_6_3","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1073\/pnas.92.8.3425","volume":"92","author":"Diekmann W.","year":"1995","unstructured":"Diekmann W., Venis M. A., Robinson D. G., Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995);","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_1_6_4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1365-313X.1991.00083.x","volume":"1","author":"Barbier-Brygoo H.","year":"1991","unstructured":"Barbier-Brygoo H., et al., Plant J. 1, 83 (1991);","journal-title":"Plant J."},{"key":"e_1_3_1_6_5","first-page":"41","volume":"4","author":"Ruck A.","year":"1993","unstructured":"Ruck A., Palme K., Venis M. A., Napier R., Felle H., ibid. 4, 41 (1993);","journal-title":"ibid."},{"key":"e_1_3_1_6_6","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1146\/annurev.pp.44.060193.002551","volume":"44","author":"Blatt M. R.","year":"1994","unstructured":"Blatt M. R., Thiel G., Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).","journal-title":"Annu. Rev. Plant Physiol. Plant Mol. Biol."},{"key":"e_1_3_1_7_2","unstructured":"The full-length Arabidopsis ABP1 cDNA was amplified from a cDNA library confirmed to be genuine by sequencing then placed in transcriptional frame to the tetracycline-derepressible promoter on the binary vector pBin-HYGRO-TX described by C. Gatz C. Frohberg and R. Wendenburg [ Plant J. 2 397 (1992)]. F 1 seed of a transgenic tobacco plant ( Nicotiana tabacum L. cv. Wisconsin 38) that overexpress the tetracycline repressor structural gene (tet R+ ) were obtained from C. Gatz (University of Gottingen Germany). F 1 progeny were screened for high-level expression of tet R by Northern (RNA) hybridization (M. A. Savka and A. N. Binns data not shown); one designated R7 which had the highest levels of Tet R transcript and was shown to be homozygous for this transgene was used for ABP1 transformations. T-DNA vectors pMJ10 (ABP1 gene construct in pBin-HYGO-TX) and vector-only plasmid pBin-HYGO-TX were used to transform R7 using standard Agrobacterium -mediated transformation of leaf disks ["},{"key":"e_1_3_1_7_3","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0076-6879(86)18105-5","volume":"118","author":"Rogers S. G.","year":"1988","unstructured":"Rogers S. G., Horsch R. B., Fraley R. T., Methods Enzymol. 118, 627 (1988);","journal-title":"Methods Enzymol."},{"key":"e_1_3_1_7_4","unstructured":"]. Shoots that developed roots in the presence of both kanamycin and hygromycin within 14 days were considered transgenic and were maintained as shoot cuttings on plantlet medium (1\/2 Murashige-Skoog 1% sucrose). Plants were selfed and ABP1 transgene homozygotes were selected. For the growth assay leaf strips were removed from leaves approximately 5 cm in length and treated for 24 hours with AhTet followed by 18 hours of 1-naphthaleneacetic acid (1-NAA) then were measured. Plants were grown under greenhouse conditions."},{"key":"e_1_3_1_8_2","unstructured":"Antiserum directed against recombinant ABP1 was generously provided by K. Palme Max Planck Institute Cologne Germany."},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1104\/pp.113.2.603","volume":"113","author":"Keller C. P.","year":"1997","unstructured":"Keller C. P., Van Volkenburgh E., Plant Physiol. 113, 603 (1997).","journal-title":"Plant Physiol."},{"key":"e_1_3_1_10_2","unstructured":"Leaf cell volume was indirectly measured as described by A. Hemerley et al. (10). R7 and MJ10B plants having approximately four fully expanded leaves were grown under normal greenhouse conditions and watered daily with 0.1% Peter's solution (Peter's Professional Marysville OH) plus or minus 4 \u03bcg\/ml AhTet for 13 days a period in excess of one plastochron. The youngest fully expanded leaf from each plant was harvested and a 0.5-mm disk of interveinal leaf tissue was punched from the midsection of the leaf (see inset of Fig. 3) using a #2 cork borer. The cell walls of these tissues were digested for 10 hours in 25 mM MES (pH 5.6) 1\/2 strength MS and Gamborg vitamins 0.4 M sucrose 1% cellulase (RS) and 0.5% macerase (R10). Just at this point 75% of cells were released from the tissue and assumed a spherical shape. Yields for protoplasts were the same for all treatments. Protoplasts were photographed and the diameters determined."},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1002\/j.1460-2075.1995.tb00064.x","volume":"14","author":"Hemerley A.","year":"1995","unstructured":"Hemerley A., et al., EMBO J. 14, 3925 (1995);","journal-title":"EMBO J."},{"key":"e_1_3_1_11_3","first-page":"438","volume":"48","author":"Foard D. E.","year":"1961","unstructured":"Foard D. E., Haber A. H., Am. J. Bot. 48, 438 (1961);","journal-title":"Am. J. Bot."},{"key":"e_1_3_1_11_4","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1086\/297061","volume":"153","author":"Kaplan D. R.","year":"1992","unstructured":"Kaplan D. R., Int. J. Plant Sci. 153, 28 (1992);","journal-title":"Int. J. Plant Sci."},{"key":"e_1_3_1_11_5","doi-asserted-by":"crossref","first-page":"693","DOI":"10.2307\/1311764","volume":"41","author":"___","year":"1991","unstructured":"___, Hagemann W., Bioscience 41, 693 (1991).","journal-title":"Bioscience"},{"key":"e_1_3_1_12_2","unstructured":"The maize ABP1 cDNA described by U. Tillmann et al. [ EMBO J. 8 2463 (1989)] was placed in transcriptional frame with the 35 S Cauliflower Mosaic viral (35 S CaMV) promoter to form vector pAJ15. Linear DNA from pAJ15 containing only the 35 S :ABP1 construction was cotransformed with linear DNA containing the same promoter driving the bar gene of Streptomyces hygroscopicus encoding phosphothricin acetyltransferase into maize protoplasts as described by R. Shillito et al. [ Biotechnology 7 581 (1989)]. Primary selection and screens of transformants was as described by C. Kramer J. DiMao G. K. Carswell and R. D. Shillito [ Planta 190 454 (1993)]. Calli surviving on plates containing phosphothricin were then subjected to a polymerase chain reaction\u2013based screen using ABP1-specific primers. Cells confirmed to be transformed were then subjected to immunoblot analyses using antibodies to maize ABP1. Cells were maintained on solid and in liquid 2N6 medium ["},{"key":"e_1_3_1_12_3","first-page":"659","volume":"18","author":"Chu C. C.","year":"1975","unstructured":"Chu C. C., et al., Sci. Sin. 18, 659 (1975)].","journal-title":"Sci. Sin."},{"key":"e_1_3_1_13_2","unstructured":"Cells (0.2 g) were collected by filtration and extracted using a 3% SDS-containing buffer. Extraction and immunoblot analysis was performed as described (4)."},{"key":"e_1_3_1_14_2","unstructured":"Cells were digested overnight in 5% cellulase (RS) and macerase (R10) at 25\u00b0C with constant agitation to produce individual cells and cells in small aggregates. The nuclei were stained with 4\u20326\u2032-diamidino-2-phenylindole and the fluorescence intensity of individual nuclei was measured using a microphotometer."},{"key":"e_1_3_1_15_2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/BF00397659","volume":"176","author":"Napier R. M.","year":"1988","unstructured":"Napier R. M., Venis M. A., Bolton M. A., Richardson L. I., Butcher G. W., Planta 176, 519 (1988).","journal-title":"Planta"},{"key":"e_1_3_1_16_2","unstructured":"Supported in part by NSF (MCB-9514306) and USDA NCRICGO (9602846) to A.M.J. and by NIH (GM47369-06) to A.N.B. Special thanks to R. Napier AFRC in UK for providing monoclonal antibodies to maize ABP1 and S. Whitfield for assistance in preparing the illustrations."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.282.5391.1114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T00:13:50Z","timestamp":1705104830000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.282.5391.1114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11,6]]},"references-count":32,"journal-issue":{"issue":"5391","published-print":{"date-parts":[[1998,11,6]]}},"alternative-id":["10.1126\/science.282.5391.1114"],"URL":"https:\/\/doi.org\/10.1126\/science.282.5391.1114","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,11,6]]}}}