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These dynamic changes in membrane fluidity represent the modulation of membrane tension by lipid-protein interactions. The voltage dependence may be associated with the force-generating motors that contribute to the exquisite sensitivity of mammalian hearing.<\/jats:p>","DOI":"10.1126\/science.287.5453.658","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:50:14Z","timestamp":1027763414000},"page":"658-661","source":"Crossref","is-referenced-by-count":105,"title":["Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane"],"prefix":"10.1126","volume":"287","author":[{"given":"John S.","family":"Oghalai","sequence":"first","affiliation":[{"name":"Department of Otolaryngology and Communicative Sciences, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Bo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology and Communicative Sciences, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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We stained the plasma membrane of the isolated cells with di-8-ANEPPS a fluorescent molecule with a short hydrophobic tail that inserts itself into the outer leaflet of the phospholipid bilayer. Di-8-ANEPPS (75 \u03bcM) was dispersed into extracellular solution by sonication for 1 min and the cells were stained for 5 min before unbound dye was thoroughly rinsed away with extracellular solution alone ["},{"key":"e_1_3_1_6_3","doi-asserted-by":"crossref","first-page":"4536","DOI":"10.1021\/bi00437a003","volume":"28","author":"Montana V.","year":"1989","unstructured":"Montana V., Farkas D. L., Loew L. M., Biochemistry 28, 4536 (1989);","journal-title":"Biochemistry"},{"key":"e_1_3_1_6_4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-5955(99)00077-5","volume":"135","author":"Oghalai J. S.","year":"1999","unstructured":"Oghalai J. S., Tran T. D., Raphael R. M., Nakagawa T., Brownell W. E., Hear. Res. 135, 19 (1999)].","journal-title":"Hear. 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For the voltage-clamp experiments up to four measurements of the diffusion coefficient were made per OHC. Each measurement was made at a different location along the lateral wall. Due to difficulties with phototoxicity it was impossible to collect a large number of data points from a single cell. We stopped using a cell when a change in the input impedance indicated either membrane breakdown or a loss of the tight seal between the recording pipette and the cell membrane. 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Laryngol."},{"key":"e_1_3_1_23_2","doi-asserted-by":"crossref","first-page":"8223","DOI":"10.1073\/pnas.96.14.8223","volume":"96","author":"He D. Z.","year":"1999","unstructured":"He D. Z., Dallos P., Proc. Natl. Acad. Sci. U.S.A. 96, 8223 (1999).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_1_24_2","unstructured":"The data were fit with a Boltzmann function D = D depolarized + ( D hyperpolarized \u2212 D depolarized )\/{1 + exp[( V \u2212 V 1\/2 )\/ s ]} where D is the diffusion coefficient at the membrane potential V D hyperpolarized and D depolarized are the diffusion coefficients at the hyperpolarized and depolarized states V 1\/2 is the potential at the midpoint of the change in D and s describes the voltage dependency of the curve (slope factor). The data were fit by computer algorithm."},{"key":"e_1_3_1_25_2","doi-asserted-by":"crossref","unstructured":"M. J. Holley in The Cochlea P. Dallos A. N. Popper R. R. Fay Eds. (Springer New York 1996) vol. 8 pp. 386\u2013434.","DOI":"10.1007\/978-1-4612-0757-3_7"},{"key":"e_1_3_1_26_2","unstructured":"All procedures were approved by the animal care and use committee at the Baylor College of Medicine. Supported by the Jake and Nina Kamin Chair and by research grants from the Deafness Research Foundation (J.S.O.) and NIDCD (W.E.B.). J.S.O. developed the techniques for measuring lateral diffusion used in this manuscript and collected the data for Figs. 1A 2 and 3. H.-B.Z. and J.W.K. collected the data for Fig. 1 B C and D. We thank B. R. Alford A. Bullen R. A. Eatock H. A. Jenkins T. Nakagawa A. Nygren A. S. Popel R. M. Raphael F. Sachs P. Saggau A. A. Spector and T. D. Tran."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.287.5453.658","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T09:08:07Z","timestamp":1705136887000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.287.5453.658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1,28]]},"references-count":44,"journal-issue":{"issue":"5453","published-print":{"date-parts":[[2000,1,28]]}},"alternative-id":["10.1126\/science.287.5453.658"],"URL":"https:\/\/doi.org\/10.1126\/science.287.5453.658","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1,28]]}}}