{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:51:17Z","timestamp":1790920277387,"version":"4.1.0"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4895","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1989,3,3]]},"abstract":"<jats:p>\n            Monoclonal antibodies have been induced that are capable of catalyzing specific hydrolysis of the Gly-Phe bond of peptide substrates at neutral\n            <jats:italic>p<\/jats:italic>\n            H with a metal complex cofactor. The antibodies were produced by immunizing with a Co(III) triethylenetetramine (trien)-peptide hapten. These antibodies as a group are capable of binding trien complexes of not only Co(III) but also of numerous other metals. Six peptides were examined as possible substrates with the antibodies and various metal complexes. Two of these peptides were cleaved by several of the antibodies. One antibody was studied in detail, and cleavage was observed for the substrates with the trien complexes of Zn(II), Ga(III), Fe(III), In(III), Cu(II), Ni(II), Lu(III), Mg(II), or Mn(II) as cofactors. A turnover number of 6 \u00d7 10\n            <jats:sup>-4<\/jats:sup>\n            per second was observed for these substrates. These results demonstrate the feasibility of the use of cofactor-assisted catalysis in an antibody binding site to accomplish difficult chemical transformations.\n          <\/jats:p>","DOI":"10.1126\/science.2922606","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T21:21:28Z","timestamp":1160083288000},"page":"1184-1188","source":"Crossref","is-referenced-by-count":166,"title":["Sequence-Specific Peptide Cleavage Catalyzed by an Antibody"],"prefix":"10.1126","volume":"243","author":[{"given":"Brent L.","family":"Iverson","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Lerner","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"5355","volume":"85","year":"1988","unstructured":"BENKOVIC, S.J., CATALYSIS OF A STEREOSPECIFIC BIMOLECULAR AMIDE SYNTHESIS BY AN ANTIBODY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 5355 (1988).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"6151","DOI":"10.1021\/ja00724a009","volume":"92","year":"1970","unstructured":"BUCKINGHAM, D.A., COBALT(III)-PROMOTED HYDROLYSIS OF GLYCINE AMIDES - INTRAMOLECULAR AND INTERMOLECULAR HYDROLYSIS FOLLOWING BASE HYDROLYSIS OF CIS- CO(EN)2BR(GLYNR1R2!2+ IONS, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 92: 6151 (1970).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"4998","DOI":"10.1021\/ja00276a048","volume":"108","year":"1986","unstructured":"CHRISTIANSON, D.W., THE COMPLEX BETWEEN CARBOXYPEPTIDASE-A AND A POSSIBLE TRANSITION-STATE ANALOG - MECHANISTIC INFERENCES FROM HIGH-RESOLUTION X-RAY STRUCTURES OF ENZYME-INHIBITOR COMPLEXES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 108: 4998 (1986).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"7888","DOI":"10.1021\/ja00231a056","volume":"110","year":"1988","unstructured":"COCHRAN, A.G., PHOTOSENSITIZED CLEAVAGE OF A THYMINE DIMER BY AN ANTIBODY, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 110: 7888 (1988).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_5_1","first-page":"3039","volume":"85","year":"1963","unstructured":"Collman, J. P., Journal of the American Chemical Society 85: 3039 (1963).","journal-title":"Journal of the American Chemical Society"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1021\/ja00315a030","volume":"106","year":"1984","unstructured":"GROVES, J.T., GEOMETRICAL AND STEREOCHEMICAL FACTORS IN METAL-PROMOTED AMIDE HYDROLYSIS, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 106: 630 (1984).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.4953"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"4841","DOI":"10.1021\/ja00222a060","volume":"110","year":"1988","unstructured":"JACKSON, D.Y., AN ANTIBODY-CATALYZED CLAISEN REARRANGEMENT, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 110: 4841 (1988).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1021\/ja00241a042","volume":"109","year":"1987","unstructured":"JACOBS, J, CATALYTIC ANTIBODIES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 109: 2174 (1987).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"4835","DOI":"10.1021\/ja00222a056","volume":"110","year":"1988","unstructured":"JANDA, K.D., ANTIBODY CATALYSIS OF BIMOLECULAR AMIDE FORMATION, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 110: 4835 (1988).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3413482"},{"key":"e_1_2_1_12_1","first-page":"64","year":"1987","unstructured":"JENCKS, W.P., CATALYSIS CHEM ENZYM: 64 (1987).","journal-title":"CATALYSIS CHEM ENZYM"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"7529","DOI":"10.1021\/ja00230a041","volume":"110","year":"1988","unstructured":"KAHNE, D, HYDROLYSIS OF A PEPTIDE-BOND IN NEUTRAL WATER, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 110: 7529 (1988).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1021\/ar00080a001","volume":"15","year":"1982","unstructured":"LIPSCOMB, W.N., ACCELERATION OF REACTIONS BY ENZYMES, ACCOUNTS OF CHEMICAL RESEARCH 15: 232 (1982).","journal-title":"ACCOUNTS OF CHEMICAL RESEARCH"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1021\/ar00153a003","volume":"21","year":"1988","unstructured":"MATHEWS, B.W., ACCOUNTS CHEM RES 21: 333 (1988).","journal-title":"ACCOUNTS CHEM RES"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3616626"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3787262"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3194752"},{"key":"e_1_2_1_19_1","first-page":"265","volume":"318","year":"1985","unstructured":"REARDEN, D.T., NATURE 318: 265 (1985).","journal-title":"NATURE"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1021\/ja00240a036","volume":"109","year":"1987","unstructured":"SCHEPARTZ, A, HYDROLYSIS OF AN AMIDE IN A CARBOXYPEPTIDASE MODEL USING CO(III) AND BIFUNCTIONAL CATALYSTS, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 109: 1814 (1987).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_21_1","first-page":"82","year":"1984","unstructured":"STEWART, J.M., SOLID PHASE PEPTIDE: 82 (1984).","journal-title":"SOLID PHASE PEPTIDE"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1021\/ja00215a045","volume":"110","year":"1988","unstructured":"TRAMONTANO, A, ANTIBODY CATALYSIS APPROACHING THE ACTIVITY OF ENZYMES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 110: 2282 (1988).","journal-title":"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY"},{"key":"e_1_2_1_23_1","first-page":"6736","volume":"83","year":"1986","unstructured":"TRAMONTANO, A, CHEMICAL-REACTIVITY AT AN ANTIBODY-BINDING SITE ELICITED BY MECHANISTIC DESIGN OF A SYNTHETIC ANTIGEN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 6736 (1986).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3787261"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.2922606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T09:15:05Z","timestamp":1705137305000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.2922606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,3,3]]},"references-count":24,"journal-issue":{"issue":"4895","published-print":{"date-parts":[[1989,3,3]]}},"alternative-id":["10.1126\/science.2922606"],"URL":"https:\/\/doi.org\/10.1126\/science.2922606","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,3,3]]}}}